Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Inactivated rabies viruses carrying canine distemper glycoproteins protect against both pathogens

02.02.2017

Currently licensed live attenuated canine distemper vaccines are highly effective. However, they retain the potential to cause disease in very susceptible species. Researchers at Paul-Ehrlich-Institut and Thomas Jefferson University have generated an inactivated candidate vaccine that induces protective immunity against both rabies and canine distemper and has an excellent safety profile. The candidate vaccine consists of a mix of two recombinant inactivated rabies viruses that carry either one of the two canine distemper glycoproteins in addition to its own glycoprotein.The results are published in the online edition of Journal of Virology 1 February 2016.

Rabies and canine distemper are among the most dangerous and lethal viral diseases afflicting carnivores. Rabies can also be transmitted to humans via bite from rabies-infected animals. It attacks the nervous system and can be deadly if not treated immediately.


Cells infected with canine distemper virus fuse to neighboring uninfected cells to form syncytia. Antibodies against canine distemper virus are used to stain the infected cells with a red dye

Source: PEI


Cells infected with rabies virus were stained with antibodies from rabies-immunized animals. These cells can be seen by stimulating a fluorescent dye attached to the antibodies, which glows green.

Source: PEI

Rabies vaccines contain inactivated virus particles and have an excellent stability and safety profile. For vaccination against canine distemper virus, which causes a measles-like disease in its hosts, live-attenuated viruses are used. These vaccine viruses replicate in the vaccinated animals to a certain extent and can lead to severe disease in highly susceptible species.

Prof. Dr. Veronika von Messling's group at the Veterinary Medicine Division, Paul-Ehrlich-Institut, in collaboration with Dr. Matthias Schnell's group at Thomas Jefferson University genetically engineered the rabies virus vaccine strain to carry one of the canine distemper virus glycoproteins in addition to its own glycoprotein. The protective immune response is directed against these proteins. Candidate vaccines were then produced following the purification and inactivation protocols used for rabies vaccines.

The researchers first demonstrated that a single shot was sufficient to elicit protective rabies antibody titers in ferrets. However, protection against canine distemper was only achieved when animals were immunized with a mix of viruses carrying both canine distemper virus glycoproteins. The researchers conclude that immune responses against both glycoproteins are necessary to protect from canine distemper.

"Our rabies-based vector is a promising platform for the development of new morbillivirus vaccines" explains von Messling. "It eliminates the risk for vaccine-induced disease and at the same time greatly increases the temperature stability compared to live-attenuated morbillivirus vaccines."

Original Publication

Da Fontoura Budaszewski R, Hudacek A, Sawatsky B, Krämer B, Xiangping Y, Schnell MJ, von Messling V (2017): Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity Against Both Pathogens.
J Virol Feb 01 [Epub ahead of print].
doi: 10.1128/JVI.02077-16


The Paul-Ehrlich-Institut, the Federal Institute for Vaccines and Biomedicines, in Langen near Frankfurt/Main is a senior federal authority reporting to the Federal Ministry of Health (Bundesministerium für Gesundheit, BMG). It is responsible for the research, assessment, and marketing authorisation of biomedicines for human use and immunological veterinary medicinal products. Its remit also includes the authorisation of clinical trials and pharmacovigilance, i.e. recording and evaluation of potential adverse effects.

Other duties of the institute include official batch control, scientific advice and inspections. In-house experimental research in the field of biomedicines and life science form an indispensable basis for the manifold tasks performed at the institute.

The Paul-Ehrlich-Institut, with its roughly 800 members of staff, also has advisory functions nationally (federal government, federal states (Länder)), and internationally (World Health Organisation, European Medicines Agency, European Commission, Council of Europe etc.).

Weitere Informationen:

http://jvi.asm.org/content/early/2017/01/26/JVI.02077-16.abstract - Abstract of the publicaton
http://www.pei.de/EN/information/journalists-press/press-releases/2017/03-inacti... - this press release on the Paul-Ehrlich-Institut Website

Dr. Susanne Stöcker | idw - Informationsdienst Wissenschaft

More articles from Health and Medicine:

nachricht Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Osaka university researchers make the slipperiest surfaces adhesive

18.10.2017 | Materials Sciences

Space radiation won't stop NASA's human exploration

18.10.2017 | Physics and Astronomy

Los Alamos researchers and supercomputers help interpret the latest LIGO findings

18.10.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>