Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Findings point to potential treatment for virus causing childhood illnesses

30.01.2014
Researchers have discovered a potential treatment for a viral infection that causes potentially fatal brain swelling and paralysis in children. The findings also point to possible treatments for related viruses including those that cause "common cold" symptoms.

The virus, called enterovirus 71 (EV71), causes yearly outbreaks of hand, foot, and mouth disease in Southeast Asian countries including China and Malaysia. Some of the infected children develop encephalitis that can be fatal or result in permanent brain damage. There are no anti-EV71therapeutic agents available.


These images illustrate antibody "footprints" outlined in yellow on the surface of a virus called EV71. Three distinct proteins - out of a total of 180 - that make up the viral capsid are outlined in blue, green, and red. The neutralizing antibodies E18 (A) and E19 (B), used in this study were found to bind to the viral proteins where the colors are bright. The E18 antibody causes the viral capsid to open, resulting in the loss of the viral genome, suggesting the virus no longer has the ability to replicate in the human host. (Department of Biological Sciences, Purdue University)

The researchers prepared antibodies by immunizing mice with immature EV71 particles. Antibodies generated by this immunization induced a change in the mature EV71 virus, rendering it non-infectious by causing the virus particles to lose their genome.

The findings reveal a potential treatment mechanism, demonstrating that the antibody, called E18, has potential as an anti-EV71 therapy, said Michael G. Rossmann, Purdue's Hanley Distinguished Professor of Biological Sciences.

Findings are being reported this week in Proceedings of the National Academy of Sciences. The paper was authored by Purdue postdoctoral research associate Pavel Plevka; Pei-Yin Lim, a researcher from Sentinext Therapeutics in Penang, Malaysia; Purdue research scientist Rushika Perera; Jane Cardosa, a researcher at Sentinext and MAB Explorations in Malaysia; Purdue graduate student Ampa Suksatu; Richard J. Kuhn, a professor and head of Purdue's Department of Biological Sciences; and Rossmann.

EV71 belongs to a family of viruses called picornaviruses, pathogens that cause diseases including common-cold-like illnesses and poliomyelitis in people and chronic infections in livestock.

"The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated," Rossmann said. "These results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles. The approach could be used to prepare antibodies with similar properties against related viruses that include significant human pathogens."

Researchers observed and documented the loss of genome using two laboratory techniques: cryoelectron microscopy and a fluorescent SYBR Green dye assay.

Hand, foot and mouth disease, an infection most common among young children, sometimes arises in a daycare setting. Of the 427,278 cases of the disease recorded in China between January and May 2010, 5,454 cases were classified as severe, with 260 deaths, according to the World Health Organization.

The research has been funded by the National Institutes of Health.

Writer: Emil Venere, 765-494-4709, venere@purdue.edu

Sources: Pavel Plevka, 765-494-8712, plevka@purdue.edu

Michael Rossmann, 765-494-4911, mr@purdue.edu

Richard J. Kuhn, 765-494-4407, kuhnr@purdue.edu

Rushika Perera, perera@purdue.edu

Note to Journalists: A copy of the research paper is available by contacting Emil Venere, 765-494-4709, venere@purdue.edu

Emil Venere | EurekAlert!
Further information:
http://www.purdue.edu

Further reports about: Biological Science EV71 Rossmann Science TV chronic infection health services

More articles from Health and Medicine:

nachricht Using DNA origami to build nanodevices of the future
31.08.2015 | Institute for Integrated Cell-Material Sciences at Kyoto University

nachricht An ounce of prevention: Research advances on 'scourge' of transplant wards
28.08.2015 | University of Wisconsin-Madison

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Increasingly severe disturbances weaken world's temperate forests

Longer, more severe, and hotter droughts and a myriad of other threats, including diseases and more extensive and severe wildfires, are threatening to transform some of the world's temperate forests, a new study published in Science has found. Without informed management, some forests could convert to shrublands or grasslands within the coming decades.

"While we have been trying to manage for resilience of 20th century conditions, we realize now that we must prepare for transformations and attempt to ease...

Im Focus: OU astrophysicist and collaborators find supermassive black holes in quasar nearest Earth

A University of Oklahoma astrophysicist and his Chinese collaborator have found two supermassive black holes in Markarian 231, the nearest quasar to Earth, using observations from NASA's Hubble Space Telescope.

The discovery of two supermassive black holes--one larger one and a second, smaller one--are evidence of a binary black hole and suggests that supermassive...

Im Focus: What would a tsunami in the Mediterranean look like?

A team of European researchers have developed a model to simulate the impact of tsunamis generated by earthquakes and applied it to the Eastern Mediterranean. The results show how tsunami waves could hit and inundate coastal areas in southern Italy and Greece. The study is published today (27 August) in Ocean Science, an open access journal of the European Geosciences Union (EGU).

Though not as frequent as in the Pacific and Indian oceans, tsunamis also occur in the Mediterranean, mainly due to earthquakes generated when the African...

Im Focus: Self-healing landscape: landslides after earthquake

In mountainous regions earthquakes often cause strong landslides, which can be exacerbated by heavy rain. However, after an initial increase, the frequency of these mass wasting events, often enormous and dangerous, declines, in fact independently of meteorological events and aftershocks.

These new findings are presented by a German-Franco-Japanese team of geoscientists in the current issue of the journal Geology, under the lead of the GFZ...

Im Focus: FIC Proteins Send Bacteria Into Hibernation

Bacteria do not cease to amaze us with their survival strategies. A research team from the University of Basel's Biozentrum has now discovered how bacteria enter a sleep mode using a so-called FIC toxin. In the current issue of “Cell Reports”, the scientists describe the mechanism of action and also explain why their discovery provides new insights into the evolution of pathogens.

For many poisons there are antidotes which neutralize their toxic effect. Toxin-antitoxin systems in bacteria work in a similar manner: As long as a cell...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Networking conference in Heidelberg for outstanding mathematicians and computer scientists

20.08.2015 | Event News

Scientists meet in Münster for the world’s largest Chitin und Chitosan Conference

20.08.2015 | Event News

Large agribusiness management strategies

19.08.2015 | Event News

 
Latest News

Production research by Fraunhofer IAO honored with three awards at the ICPR 2015

31.08.2015 | Awards Funding

Single-Crystal Phosphors Suitable for Ultra-Bright, High-Power White Light Sources

31.08.2015 | Materials Sciences

Manchester Team Reveal New, Stable 2D Materials

31.08.2015 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>