Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

EHEC: Scientists of Münster publish in PLoS ONE their prospective genomic characterization

21.07.2011
Analysis was performed during the outbreak in Germany

Scientists of the Medical Faculty of the University Münster and the University Hospital Münster in collaboration with scientists of the enterprise `Life Technologies Corporation´ were the first to release a draft genome sequence of a German enterohemorrhagic E. coli (EHEC) 2011 outbreak strain on June 3rd. Their in-depth genomic characterization of this outbreak was published on July 20th in the online open access journal PLoS ONE.

Microbiologist Prof. Dr. Dag Harmsen from the Department of Periodontology in Münster – the corresponding author of this publication - is leading a team responsible for sequencing and conducting the bioinformatics analysis in Münster. "Thanks to the Ion Torrent PGM™ next generation sequencing (NGS) platform we were very quick. In essence this is the first demonstration where NGS was used in real-time for genomic outbreak analysis. There have already been some publications using NGS to retrospectively analyze outbreaks, but analysis during an ongoing outbreak had not yet been performed (figure). Basically a new discipline is born, i.e. prospective genomics epidemiology," he explains.

Such rapid sequencing is to be considered a "technical masterpiece, which will have immediate impact on surveillance and diagnostics and most probably in the future also on therapeutics", says Prof. Dr. Wilhelm Schmitz, Dean of the Medical Faculty of the University Münster.

"By comparing the EHEC O104:H4 outbreak genome with a simultaneously sequenced genome of an EHEC O104:H4 isolate from an HUS patient (isolated in Germany in 2001; the HUSEC041 reference strain), we were able to demonstrate that the current outbreak strain was not– as initially suggested - derived from the very similar enteroaggregative E. coli (EAEC) O104:H4 55989 strain but from a yet unknown Shiga toxin-producing O104:H4 progenitor strain", said Dr. Alexander Mellmann of the `National Consulting Laboratory for Hemolytic Uremic Syndrome (HUS) at the Institute of Hygiene, Münster.

The senior author of the PLoS ONE study and head of the Institute of Hygiene, Prof. Dr. h.c. Helge Karch, added, “This study underlined the great importance of the long-term storage of historical HUS isolates that were collected by us since 1996 to understand the evolution of highly-pathogenic EHEC strains.”

Citation:

Mellmann A*, Harmsen D*, Cummings CA*, Zentz EB, Leopold SR, et al. (2011) Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology. PLoS ONE 6(7): e22751. doi:10.1371/journal.pone.0022751

*These authors contributed equally to this work.

Stefan Dreising | idw
Further information:
http://www.ukmuenster.de

Further reports about: E. coli EHEC German language HUS Medical Wellness NGS PLoS One oral hygiene outbreak strain

More articles from Health and Medicine:

nachricht Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>