Stiffness drives cancer invasion through previously unknown mechanism
Researchers at the University of California, San Diego School of Medicine and Moores Cancer Center have discovered a molecular mechanism that connects breast tissue stiffness to tumor metastasis and poor prognosis. The study, published April 20 in Nature Cell Biology, may inspire new approaches to predicting patient outcomes and halting tumor metastasis.
"We're finding that cancer cell behavior isn't driven by just biochemical signals, but also biomechanical signals from the tumor's physical environment," said senior author Jing Yang, PhD, associate professor of pharmacology and pediatrics.
In breast cancer, dense clusters of collagen fibers makes the tumor feel stiffer than surrounding tissue. That's why breast tumors are most often detected by touch -- they feel harder than normal breast tissue. But it's also known that increased tumor stiffness correlates with tumor progression and metastasis, as well as poor survival.
To determine how tissue stiffness influences tumor behavior, the team of cancer biologists and bioengineers used a hydrogel system to vary the rigidity on 3D cultures of breast cells from that typically experienced by normal mammary glands to the high stiffness characteristic of breast tumors. They discovered that high stiffness causes a protein called TWIST1 to lose its molecular anchor and move into the cell's nucleus. In the nucleus, TWIST1 activates genes that enable breast cancer cells to invade surrounding tissue and metastasize to other places in the body.
The researchers also compared mouse models of human breast cancer with and without TWIST1's anchor, a protein called G3BP2. Without G3BP2, tumors were more invasive and developed more metastases in the lung, compared to tumors with G3BP2.
The same mechanism plays out in human breast cancers, too, the researchers found. Analysis of human breast cancer patient samples showed that patients with stiffer tumors (meaning tumors with more organized collagen structures) did not survive as long as patients with more compliant tumors with disorganized collagen. Patients with both low G3BP2 and stiffer tumors had even shorter survival times. The correlations were so clear the team could use these factors -- G3BP2 protein levels and collagen organization -- to predict patient outcome.
"Next we want to understand exactly how cells interpret mechanical cues into biological responses," said Laurent Fattet, PhD, a postdoctoral researcher in Yang's lab who led the study, along with former graduate student Spencer Wei. "This cross-talk between a tumor's biomechanical microenvironment and the inter-workings of individual cancer cells may someday provide new therapeutic strategies to slow cancer's spread."
Co-authors of this study also include Jeff H. Tsai, Vincent H. Pai, Hannah E. Majeski, Albert C. Chen, Robert L. Sah, and Adam J. Engler, UC San Diego; Yurong Guo, and Susan S. Taylor, Howard Hughes Medical Institute and UC San Diego.
This research was funded, in part, by the National Institutes of Health (grants DP2OD002420-01, 1RO1CA168689, 1R01CA174869, DK54441, P01AG007996, 2T32CA067754, 5T32CA077109), Department of Defense Breast Cancer Program, American Cancer Society, Howard Hughes Medical Institute, ARCS Foundation and Fondation pour la Recherche Médicale.
Heather Buschman | EurekAlert!
Study suggests possible new target for treating and preventing Alzheimer's
02.12.2016 | Oregon Health & Science University
The first analysis of Ewing's sarcoma methyloma opens doors to new treatments
01.12.2016 | IDIBELL-Bellvitge Biomedical Research Institute
Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water
In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
05.12.2016 | Power and Electrical Engineering
05.12.2016 | Information Technology
05.12.2016 | Earth Sciences