Los Alamos and partners test ionic liquids to break bacterial biofilm layer and save lives
Like a protective tent over a colony of harmful bacteria, biofilms make the treatment of skin infections especially difficult. Microorganisms protected in a biofilm pose a significant health risk due to their antibiotic resistance and recalcitrance to treatment, and biofilm-protected bacteria account for some 80 percent of total bacterial infections in humans and are 50 to 1,000 times more resistant to antibiotics than simpler bacterial infections.
Artist’s rendition of a cross section of skin layers (stratum corneum, epidermis and dermis) showing topical application of an ionic liquid for combating a skin-borne bacterial infection. The ionic liquid can be formulated with antibiotics for transdermal drug delivery or it can directly kill the bacteria infesting the skin surface. (Credit: Peter Allen, UCSB)
“In essence, we may have stumbled onto a magic bullet,” said David Fox, a Los Alamos National Laboratory researcher on the project. “Through a robust screening strategy, our research team has identified a unique class of materials, known as ionic liquids, which both neutralize biofilm-forming pathogens and deliver drugs through the skin,” he said.
“We extended our current capability in antimicrobial platforms with ionic liquids to new heights by partnering with Dr. Mitragotri at UCSB, who is an expert in transdermal drug delivery platforms. The merger made perfect sense,” stated Fox.
“In several cases, we found the ionic liquid was more efficacious on a biofilm than a standard bleach treatment and exhibited minimal cytotoxicity effects on human cell lines (unlike bleach). This has excellent prospects for aiding antibiotic delivery to the pathogen through biofilm disruption but, most interestingly, the ionic liquids themselves are quite effective for pathogen neutralization,” Fox said.
This work could have especially useful applications for military medical treatments, he noted, where soldiers in the field can be exposed to bacterial infections that are particularly difficult to treat.
Biofilms often persist in the periphery of an actual wound, beneath an intact, healthy skin layer and the difficulty of their treatment is largely due to the outermost layer of the skin, the stratum corneum, being a natural barrier for drug delivery.
“If the bacterial biofilm can be disrupted, delivery of antibiotics is greatly enhanced, and any dispersed pathogens are generally restored to normal antibiotic susceptibility,” said Fox. “Further, many bacterial infestations in wounds penetrate under the outer skin layer, the stratum corneum, and deep into the tissue (epidermis and dermis). These materials are able to penetrate through the skin and effectively carry antibiotics to the deepest layers.”
“Clearly, the ionic liquids would be of special benefit to our warfighters where exposure to biological agents in hostile environments is likely. Topical application as a prophylaxis or direct treatment to an open wound could buy enough time to reach the proper medical facilities when in an austere environment,” he said. Importantly, ionic liquids can be derived from very cheap starting materials that are FDA approved and are extremely stable to high temperatures and pressures, which are necessary traits for commercialization in real-world applications.
In a groundbreaking manuscript appearing this week in the Proceedings of the National Academy of Science, as part of a multi-institutional effort between Los Alamos, University of California Santa Barbara, Dixie State University and Northern Arizona University, researchers explored exploiting ionic liquids both in a concerted effort to combat antibiotic-resistant bacterial biofilms in skin, as well as for topical transdermal drug delivery. The comprehensive strategy resulted in the identification of ionic liquids that are effective at disrupting biofilms, neutralizing pathogens, and enhancing delivery of antibiotic into skin.
Biofilms are a major cause of chronic wounds and wound degeneration. Wounds from infected surgical incisions result in 1 million additional hospital days. Additional causes of bacterial infected wounds include traumatic injuries, as well as diabetic foot ulcers, venous leg ulcers, and pressure ulcers.
The total economic burden of skin disease was estimated to be approximately $96 billion in 2004, and the prevalence and healthcare costs for skin disease have been increasing over the last three decades. Bacterial infections in the skin are among the most common diagnoses in hospital patients, accounting for some 10% of all hospital visits. Staphylococcus aureus infections acquired in hospitals, which account for only 16% of nosocomial infections, are estimated to result in $9.5 billion in extra patient costs and 12,000 deaths annually.
The comprehensive approach is unique in that the team examined a panel of in-house synthesized ionic liquids and enabled the discovery of one ionic liquid, choline-geranate, which showed excellent antimicrobial activity, minimal toxicity to epithelial cells as well as skin, and effective permeation enhancement for drug delivery. Specifically, choline-geranate was comparable with, or more effective than, bleach treatment against established biofilms of Salmonella enterica and Pseudomonas aeruginosa, respectively. In addition, choline-geranate increased delivery of cefadroxil, an antibiotic, by >16-fold into the deep tissue layers of the skin without inducing skin irritation.
Authors: Michael Zakrewsky, Vivian Le, Samir Mitragotri (University of California, Santa Barbara), Katherine S. Lovejoy, Theresa L. Kern, Tarryn E. Miller, Amber Nagy, Andrew M. Goumas, Rashi S. Iyer, David T. Fox (Los Alamos National Laboratory), Rico E. Del Sesto (Dixie State University, St. George, UT), and Andrew T. Koppisch (Northern Arizona University, Flagstaff, AZ).
Funding: This research was funded by University of California, Office of the President Grant 12-LR-237080. FTIR was performed in the Materials Research Laboratory (MRL) Shared Experimental Facilities, supported by the Materials Research Science and Engineering Centers Program of the National Science Foundation (NSF) under Award DMR 1121053. A patent application has been filed on the syntheses and biological applications of the ionic liquids.
Los Alamos National Laboratory, a multidisciplinary research institution engaged in strategic science on behalf of national security, is operated by Los Alamos National Security, LLC, a team composed of Bechtel National, the University of California, The Babcock & Wilcox Company, and URS for the Department of Energy's National Nuclear Security Administration.
Los Alamos enhances national security by ensuring the safety and reliability of the U.S. nuclear stockpile, developing technologies to reduce threats from weapons of mass destruction, and solving problems related to energy, environment, infrastructure, health, and global security concerns.
Nancy Ambrosiano | Eurek Alert!
Mobile phone test can reveal vision problems in time
11.02.2016 | University of Gothenburg
Proteomics and precision medicine
08.02.2016 | University of Iowa Health Care
Today, plants and microorganisms are heavily used for the production of medicinal products. The production of biopharmaceuticals in plants, also referred to as “Molecular Pharming”, represents a continuously growing field of plant biotechnology. Preferred host organisms include yeast and crop plants, such as maize and potato – plants with high demands. With the help of a special algal strain, the research team of Prof. Ralph Bock at the Max Planck Institute of Molecular Plant Physiology in Potsdam strives to develop a more efficient and resource-saving system for the production of medicines and vaccines. They tested its practicality by synthesizing a component of a potential AIDS vaccine.
The use of plants and microorganisms to produce pharmaceuticals is nothing new. In 1982, bacteria were genetically modified to produce human insulin, a drug...
Atomic clock experts from the Physikalisch-Technische Bundesanstalt (PTB) are the first research group in the world to have built an optical single-ion clock which attains an accuracy which had only been predicted theoretically so far. Their optical ytterbium clock achieved a relative systematic measurement uncertainty of 3 E-18. The results have been published in the current issue of the scientific journal "Physical Review Letters".
Atomic clock experts from the Physikalisch-Technische Bundesanstalt (PTB) are the first research group in the world to have built an optical single-ion clock...
The University of Würzburg has two new space projects in the pipeline which are concerned with the observation of planets and autonomous fault correction aboard satellites. The German Federal Ministry of Economic Affairs and Energy funds the projects with around 1.6 million euros.
Detecting tornadoes that sweep across Mars. Discovering meteors that fall to Earth. Investigating strange lightning that flashes from Earth's atmosphere into...
Physicists from Saarland University and the ESPCI in Paris have shown how liquids on solid surfaces can be made to slide over the surface a bit like a bobsleigh on ice. The key is to apply a coating at the boundary between the liquid and the surface that induces the liquid to slip. This results in an increase in the average flow velocity of the liquid and its throughput. This was demonstrated by studying the behaviour of droplets on surfaces with different coatings as they evolved into the equilibrium state. The results could prove useful in optimizing industrial processes, such as the extrusion of plastics.
The study has been published in the respected academic journal PNAS (Proceedings of the National Academy of Sciences of the United States of America).
Exceeding critical temperature limits in the Southern Ocean may cause the collapse of ice sheets and a sharp rise in sea levels
A future warming of the Southern Ocean caused by rising greenhouse gas concentrations in the atmosphere may severely disrupt the stability of the West...
12.02.2016 | Event News
09.02.2016 | Event News
02.02.2016 | Event News
12.02.2016 | Physics and Astronomy
12.02.2016 | Life Sciences
12.02.2016 | Medical Engineering