Studies have suggested that exposure to minimal doses of blue-violet light (400-500 nm) elicits production of small amounts of reactive oxygen species (ROS), and contributes to increased mitochondrial activity and cell growth in epithelial cells. Many growth factor signaling pathways generate ROS.
In this study, Millan and other researchers involved in this study hypothesize that exposure to blue-violet light may enhance cell growth. To test this hypothesis, they developed a novel in-vitro wound healing model that allowed them to monitor the cellular responses to a single, small dose of light in cultured cells.
Normal human epidermal keratinocytes (NHEK) and human gingival fibroblasts (HGF) were plated around cloning cylinders. At confluency, the cylinders were removed to create a wound. Cells were treated with a single 5 J/cm2 light dose delivered by a quartz-tungsten-halogen light source. Mitochondrial succinate dehydrogenase activity was measured via a standard MTT assay, and cell proliferation was assessed using DRAQ5 DNA dye. Conditioned media were collected at each time point and used in a growth factor antibody array (RayBio®) to compare the secretion products.
Growth factor array results showed that NHEK responded to blue light exposure by increasing secretion of several growth factors including insulin-like growth factor binding protein-1, amphiregulin, epidermal growth factor and fibroblast growth factor-b. Likewise, mitochondrial dehydrogenase activity and cell proliferation were enhanced in NHEK. In contrast, HGF did not respond to blue light exposure significantly in any of the parameters tested.
These results show that NHEK cells responded robustly to a single, small dose of light by increasing their mitochondrial activity, DNA synthesis, and production of growth factors. Together, these data suggest that blue light may be useful to enhance epithelial cell growth in a wound site.
This is a summary of abstract #488, "A Novel In Vitro Model for Light-Induced Wound Healing," to be presented by C. Millan at 3:30 p.m. on Thursday, March 4, 2010, in Exhibit Hall D of the Walter E. Washington Convention Center, during the 39th Annual Meeting of the American Association for Dental Research.
About the American Association for Dental Research
The American Association for Dental Research (AADR), headquartered in Alexandria, Va., is a nonprofit organization with nearly 4,000 members in the United States. Its mission is: (1) to advance research and increase knowledge for the improvement of oral health; (2) to support and represent the oral health research community; and (3) to facilitate the communication and application of research findings. AADR is the largest Division of the International Association for Dental Research (IADR).
To learn more about the AADR, visit www.aadronline.org.
Ingrid Thomas | EurekAlert!
3D images of cancer cells in the body: Medical physicists from Halle present new method
16.05.2018 | Martin-Luther-Universität Halle-Wittenberg
Better equipped in the fight against lung cancer
16.05.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...
A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.
The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...
Cardiovascular tissue engineering aims to treat heart disease with prostheses that grow and regenerate. Now, researchers from the University of Zurich, the Technical University Eindhoven and the Charité Berlin have successfully implanted regenerative heart valves, designed with the aid of computer simulations, into sheep for the first time.
Producing living tissue or organs based on human cells is one of the main research fields in regenerative medicine. Tissue engineering, which involves growing...
A team of scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg investigated optically-induced superconductivity in the alkali-doped fulleride K3C60under high external pressures. This study allowed, on one hand, to uniquely assess the nature of the transient state as a superconducting phase. In addition, it unveiled the possibility to induce superconductivity in K3C60 at temperatures far above the -170 degrees Celsius hypothesized previously, and rather all the way to room temperature. The paper by Cantaluppi et al has been published in Nature Physics.
Unlike ordinary metals, superconductors have the unique capability of transporting electrical currents without any loss. Nowadays, their technological...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
18.05.2018 | Power and Electrical Engineering
18.05.2018 | Information Technology
18.05.2018 | Information Technology