Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A new method for testing allergenic substances without experimental animals

21.12.2011
Contact allergy affects around 20% of the population in the western world. Scientists are working intensively to develop alternative test methods that do not require animal testing. A research group at the University of Gothenburg, Sweden, has now developed a unique test method that enables graded results to be obtained using cultured skin cells.

"We have made several discoveries about the mechanism behind contact allergy, one of which is that allergenic substances react with keratin 5 and 14 in the skin. The skin cells form what are known as "blebs" when exposed to allergenic substances, and this can be used to test whether a substance is allergenic", says Sofia Andersson from the Department of Chemistry at the University of Gothenburg.

Contact allergy affects around 20% of the population in the western world. Metals such as nickel, and substances in perfume and preservatives are among the most common allergenic substances. They are often components of products that are in contact with the skin, such as jewellery, skin lotions and make up. Contact allergy cannot be cured, and an affected person must avoid the allergenic substance, in order to avoid troublesome eczema. This can be a problem if the substance is present in many different products. It is for this reason important to test cosmetic products in order to prevent the development of contact allergy.

The EU has now forbidden the testing of cosmetics and their ingredients on animals. It is, however, not a simple matter to fully replace animal tests. Until now, animal-free tests have only been able to determine whether a substance is allergenic or not – such tests have not been able to determine the extent to which a substance causes allergy. Thus, much work remains to be done in developing alternative methods.

"Cultured skin cells are exposed to substances for 24 hours in our test, and then photographed. The number of cells with blebs is then counted. The greater the number of blebbing cells, the more powerful is the allergenic potential of the substance. Thus, our new test has the potential to give a graded reply: it can quite simply determine whether an allergenic substance is extremely, strongly, moderately or weakly allergenic", says Sofia Andersson.

The results can then be used to determine safe concentrations of substances in products that are used in contact with the skin. Since the experiments have given very promising results, the scientists are now working together with GU Holding in developing the test and the analysis method.

The thesis Contact Sensitizers Induce Keratinocytes to Release Epitopes - Tools for In Vitro Tests and Implications for Autoimmunity was successfully defended at a disputation held at the University of Gothenburg.

Sofia Andersson | EurekAlert!
Further information:
http://www.gu.se

Further reports about: Gothenburg animal-free tests contact allergy skin cell skin lotions

More articles from Health and Medicine:

nachricht A promising target for kidney fibrosis
21.04.2017 | Brigham and Women's Hospital

nachricht Stem cell transplants: activating signal paths may protect from graft-versus-host disease
20.04.2017 | Technische Universität München

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

7th International Conference on Crystalline Silicon Photovoltaics in Freiburg on April 3-5, 2017

03.04.2017 | Event News

 
Latest News

Molecular libraries for organic light-emitting diodes

24.04.2017 | Life Sciences

Research sheds new light on forces that threaten sensitive coastlines

24.04.2017 | Earth Sciences

Making lightweight construction suitable for series production

24.04.2017 | Machine Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>