Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A new method for testing allergenic substances without experimental animals

21.12.2011
Contact allergy affects around 20% of the population in the western world. Scientists are working intensively to develop alternative test methods that do not require animal testing. A research group at the University of Gothenburg, Sweden, has now developed a unique test method that enables graded results to be obtained using cultured skin cells.

"We have made several discoveries about the mechanism behind contact allergy, one of which is that allergenic substances react with keratin 5 and 14 in the skin. The skin cells form what are known as "blebs" when exposed to allergenic substances, and this can be used to test whether a substance is allergenic", says Sofia Andersson from the Department of Chemistry at the University of Gothenburg.

Contact allergy affects around 20% of the population in the western world. Metals such as nickel, and substances in perfume and preservatives are among the most common allergenic substances. They are often components of products that are in contact with the skin, such as jewellery, skin lotions and make up. Contact allergy cannot be cured, and an affected person must avoid the allergenic substance, in order to avoid troublesome eczema. This can be a problem if the substance is present in many different products. It is for this reason important to test cosmetic products in order to prevent the development of contact allergy.

The EU has now forbidden the testing of cosmetics and their ingredients on animals. It is, however, not a simple matter to fully replace animal tests. Until now, animal-free tests have only been able to determine whether a substance is allergenic or not – such tests have not been able to determine the extent to which a substance causes allergy. Thus, much work remains to be done in developing alternative methods.

"Cultured skin cells are exposed to substances for 24 hours in our test, and then photographed. The number of cells with blebs is then counted. The greater the number of blebbing cells, the more powerful is the allergenic potential of the substance. Thus, our new test has the potential to give a graded reply: it can quite simply determine whether an allergenic substance is extremely, strongly, moderately or weakly allergenic", says Sofia Andersson.

The results can then be used to determine safe concentrations of substances in products that are used in contact with the skin. Since the experiments have given very promising results, the scientists are now working together with GU Holding in developing the test and the analysis method.

The thesis Contact Sensitizers Induce Keratinocytes to Release Epitopes - Tools for In Vitro Tests and Implications for Autoimmunity was successfully defended at a disputation held at the University of Gothenburg.

Sofia Andersson | EurekAlert!
Further information:
http://www.gu.se

Further reports about: Gothenburg animal-free tests contact allergy skin cell skin lotions

More articles from Health and Medicine:

nachricht Vanishing capillaries
23.03.2017 | Technische Universität München

nachricht How prenatal maternal infections may affect genetic factors in Autism spectrum disorder
22.03.2017 | University of California - San Diego

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

When Air is in Short Supply - Shedding light on plant stress reactions when oxygen runs short

23.03.2017 | Life Sciences

Researchers use light to remotely control curvature of plastics

23.03.2017 | Power and Electrical Engineering

Sea ice extent sinks to record lows at both poles

23.03.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>