Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A new method for testing allergenic substances without experimental animals

21.12.2011
Contact allergy affects around 20% of the population in the western world. Scientists are working intensively to develop alternative test methods that do not require animal testing. A research group at the University of Gothenburg, Sweden, has now developed a unique test method that enables graded results to be obtained using cultured skin cells.

"We have made several discoveries about the mechanism behind contact allergy, one of which is that allergenic substances react with keratin 5 and 14 in the skin. The skin cells form what are known as "blebs" when exposed to allergenic substances, and this can be used to test whether a substance is allergenic", says Sofia Andersson from the Department of Chemistry at the University of Gothenburg.

Contact allergy affects around 20% of the population in the western world. Metals such as nickel, and substances in perfume and preservatives are among the most common allergenic substances. They are often components of products that are in contact with the skin, such as jewellery, skin lotions and make up. Contact allergy cannot be cured, and an affected person must avoid the allergenic substance, in order to avoid troublesome eczema. This can be a problem if the substance is present in many different products. It is for this reason important to test cosmetic products in order to prevent the development of contact allergy.

The EU has now forbidden the testing of cosmetics and their ingredients on animals. It is, however, not a simple matter to fully replace animal tests. Until now, animal-free tests have only been able to determine whether a substance is allergenic or not – such tests have not been able to determine the extent to which a substance causes allergy. Thus, much work remains to be done in developing alternative methods.

"Cultured skin cells are exposed to substances for 24 hours in our test, and then photographed. The number of cells with blebs is then counted. The greater the number of blebbing cells, the more powerful is the allergenic potential of the substance. Thus, our new test has the potential to give a graded reply: it can quite simply determine whether an allergenic substance is extremely, strongly, moderately or weakly allergenic", says Sofia Andersson.

The results can then be used to determine safe concentrations of substances in products that are used in contact with the skin. Since the experiments have given very promising results, the scientists are now working together with GU Holding in developing the test and the analysis method.

The thesis Contact Sensitizers Induce Keratinocytes to Release Epitopes - Tools for In Vitro Tests and Implications for Autoimmunity was successfully defended at a disputation held at the University of Gothenburg.

Sofia Andersson | EurekAlert!
Further information:
http://www.gu.se

Further reports about: Gothenburg animal-free tests contact allergy skin cell skin lotions

More articles from Health and Medicine:

nachricht Study suggests possible new target for treating and preventing Alzheimer's
02.12.2016 | Oregon Health & Science University

nachricht The first analysis of Ewing's sarcoma methyloma opens doors to new treatments
01.12.2016 | IDIBELL-Bellvitge Biomedical Research Institute

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

Im Focus: Molecules change shape when wet

Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water

In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...

Im Focus: Fraunhofer ISE Develops Highly Compact, High Frequency DC/DC Converter for Aviation

The efficiency of power electronic systems is not solely dependent on electrical efficiency but also on weight, for example, in mobile systems. When the weight of relevant components and devices in airplanes, for instance, is reduced, fuel savings can be achieved and correspondingly greenhouse gas emissions decreased. New materials and components based on gallium nitride (GaN) can help to reduce weight and increase the efficiency. With these new materials, power electronic switches can be operated at higher switching frequency, resulting in higher power density and lower material costs.

Researchers at the Fraunhofer Institute for Solar Energy Systems ISE together with partners have investigated how these materials can be used to make power...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

UTSA study describes new minimally invasive device to treat cancer and other illnesses

02.12.2016 | Medical Engineering

Plasma-zapping process could yield trans fat-free soybean oil product

02.12.2016 | Agricultural and Forestry Science

What do Netflix, Google and planetary systems have in common?

02.12.2016 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>