Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A human respiratory tissue model to assess the toxicity of inhaled chemicals and pollutants

26.03.2015

An extensive analysis shows that MucilAir™ cells maintain normal biochemical processes for at least six months

A 3-dimensional model of human respiratory tissue has been shown to be an effective platform for measuring the impact of chemicals, like those found in cigarette smoke, or other aerosols on the lung.


Schematic representation of the MucilAir™ pseudostratied respiratory epithelium grown on the surface of a porous membrane with culture medium on the basal side and air on the apical side. The goblet cells secrete mucin proteins in a matrix composed of water, complex sugars, salts which form the mucus. This matrix is distributed evenly on the surface of the epithelium by the ciliated cells to form a protective layer. Basal cells are undierentiated cells with the potential to divide to form new goblet cells and new ciliated cells. Inhaled chemicals (toxicants, medicine) can be metabolized by the tissue and excreted in the basal media.

Credit: BAT

Effective lab-based tests are required to eliminate the need for animal testing in assessing the toxicological effects of inhaled chemicals and safety of medicines. Traditional lab-based tests use cell lines that do not reflect normal lung structure and physiology, and in some cases have reduced, or loss of, key metabolic processes.

Consequently, the long-term toxicological response of the cells can differ from what actually happens in humans. Since the damaging effect of inhaled toxicants usually results from repeated exposures to low doses over a prolonged period, it is important that cell culture systems maintain their physiology, in particular the ability to metabolise chemicals over time.

Scientists at British American Tobacco assessed a commercially available 3-dimensional in vitro tissue model, MucilAir™ by growing human respiratory epithelial cells at the air-liquid interface on a porous membrane - in this case nasal cells were used. The results show that the cells in the test model remain viable for at least six months, which makes this tissue model suitable for testing the impact of repeated exposures over a prolonged period, as in the case of the typical smoker. This results of this study will be published in Toxicology in Vitro.

The researchers maintained MucilAir™ in culture for six months, during which they periodically measured biomarkers that are key characteristics of normal respiratory epithelium. These included mucus secretion, cilia beat frequency, metabolic enzyme activity and gene expression.

The researchers compared the results with those from standard human sputum and metabolic gene expression in normal human lung. They found that MucilAir™ cells retain normal metabolic gene expression and key protein markers typical of the respiratory epithelium. When the MucilAir™ cultures were assessed over a period of six months, no significant changes in the expression profile of the tested proteins, metabolic genes, and metabolic activity was detected- confirming the suitability of MucilAir™ for use in long-term toxicological testing.

"The combined weight of evidence from proteomics, gene expression and protein activity demonstrates that the MucilAir™ system is far better than continuous cell lines for assessing the effect of repeated exposure to inhaled chemicals and toxicants," says senior scientist Emmanuel Minet. "Cell lines have been extensively used in research, but suffer from a key problem; they are often derived from diseased tissues and don't have normal characteristics, hence the need to use more physiologically relevant systems," he said. "Our results give clear supporting evidence that MucilAir™ has the potential to be used to test not only the effect of acute doses of toxicants and medicines but also the effect of repeated doses over time."

The researchers plan to use the model to compare the toxicological effect of repeated exposures to aerosols generated from conventional and next-generation tobacco and nicotine products. This research will be published in Toxicology in Vitro.

Media Contact

Marina Murphy
marina_murphy@bat.com
44-077-111-59135

 @BATPress

http://www.bat-science.com 

Marina Murphy | EurekAlert!

Further reports about: 3-dimensional Toxicology activity doses epithelium lung metabolic pollutants toxicity

More articles from Health and Medicine:

nachricht New malaria analysis method reveals disease severity in minutes
14.08.2017 | University of British Columbia

nachricht New type of blood cells work as indicators of autoimmunity
14.08.2017 | Instituto de Medicina Molecular

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

Gold shines through properties of nano biosensors

17.08.2017 | Physics and Astronomy

Greenland ice flow likely to speed up: New data assert glaciers move over sediment, which gets more slippery as it gets wetter

17.08.2017 | Earth Sciences

Mars 2020 mission to use smart methods to seek signs of past life

17.08.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>