Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Maglev tissues could speed toxicity tests

25.01.2013
Scientists use magnetic levitation to make in vitro lung tissue more realistic

In a development that could lead to faster and more effective toxicity tests for airborne chemicals, scientists from Rice University and the Rice spinoff company Nano3D Biosciences have used magnetic levitation to grow some of the most realistic lung tissue ever produced in a laboratory.

The research is part of an international trend in biomedical engineering to create laboratory techniques for growing tissues that are virtually identical to those found in people's bodies. In the new study, researchers combined four types of cells to replicate tissue from the wall of the bronchiole deep inside the lung.

The research is available online and scheduled to appear in a future issue of the journal Tissue Engineering Part C: Methods.

"One of the unique things about the magnetic levitation technology is that it allows us to move cells around and arrange them the way that we want for a particular types of tissue," said study co-author Tom Killian, professor and department chair of physics and astronomy at Rice. "This is the first time anyone has arranged these four cell types in the same way that they are found in lung tissue."

In vitro laboratory tests have historically been conducted on 2-D cell cultures grown in flat petri dishes, but scientists have become increasingly aware that cells in flat cultures sometimes behave and interact differently than cells that are immersed in 3-D tissue.

Killian and fellow scientists from Rice and the University of Texas MD Anderson Cancer Center co-founded Nano3D Biosciences in 2009 after creating a technology that uses magnetism to levitate and grow 3-D cell cultures. The technology relies on inert, nontoxic magnetic nanoparticles that are inserted into the living cells. Researchers can then use magnets to lift and suspend the cells as they grow and divide.

"Growing realistic lung tissues in vitro is a particular challenge," said study co-author Jane Grande-Allen, professor of bioengineering at Rice. "There are a number of technical obstacles, and scientific funding agencies have placed a particular emphasis on lung tissue because there's a large potential payoff in terms of reducing costs for pharmaceutical and toxicological testing."

Nano3D Biosciences won a Small Business Innovation Research (SBIR) grant from the National Science Foundation (NSF) in 2011 to create a four-layered lung tissue from endothelial cells, smooth muscle cells, fibroblasts and epithelial cells.

Glauco Souza, the company's chief scientific officer and co-founder, said the project switched into high gear when Rice bioengineering graduate student Hubert Tseng joined the research team as an intern. Tseng was already a student in Grande-Allen's lab, one of Rice's leading laboratories for tissue-engineering research.

"Hubert's and Jane's expertise in tissue engineering was invaluable for tackling this problem," Souza said.

Another collaboration that paid off big was a partnership with a group of undergraduate students at Rice's Oshman Engineering Design Kitchen. The undergraduate team, Cells in 3-D, worked on a magnetic pen that could be used to grab, move and combine magnetized 3-D cell cultures. Souza said Tseng used a version of this tool to create layered bronchiole tissues for this new study.

Tseng said the new tissue resembles native bronchiole tissue more closely than any other tissue yet created in the lab.

"We conducted a number of tests, and the tissue has the same biochemical signature as native tissue," Tseng said. "We also used primary cells rather than engineered cells, which is important for toxicological testing because primary cells provide the closest possible match to native cells."

Souza said bronchiole tissue could solve another problem that's frequently encountered in testing the toxicity of airborne agents.

"With traditional 2-D cultures, it is very difficult to culture cells at the air-liquid interface, which is what you'd prefer for toxicity testing," he said. "With our technology, we can easily levitate the bronchiole tissue to the air-liquid interface so that airborne toxins are exposed to the epithelial layer of the tissue, just as it would occur in the lungs."

Grande-Allen said Tseng and other members of her group have already used the same methods pioneered in the bronchiole study to produce heart valve tissue; Souza said the NSF has awarded the company with a second phase of SBIR funding to further develop the technique for other types of tissue.

Study co-authors include Robert Raphael, professor of bioengineering at Rice and co-founder of Nano3D Biosciences; Dr. Robert Moore, a pediatric pulmonologist at Baylor College of Medicine (BCM); and former BCM scientist Jacob Gage, now with Nano3D Biosciences.

The research was funded by NSF and the Texas Emerging Technologies Fund.

High-resolution IMAGES are available for download at: http://news.rice.edu/wp-content/uploads/2013/01/0128-LUNG-all4-lg.jpg

CAPTION: This composite shows 3-D cultures of four types of cells that Rice University scientists combined in vitro to create bronchiole lung tissue. The cells are: epithelial cells (EpiC), smooth muscle cells (SMC), pulmonary fibroblasts (HPF) and pulmonary endothelial cells (PEC).

CREDIT: Hubert Tseng/Rice University

http://news.rice.edu/wp-content/uploads/2013/01/0128-LUNG-slide-lg.jpg

CAPTION: This microscopic image shows the structure and layers of in vitro bronchiole tissue created at Rice University and Nano3D Biosciences. The cell layers include epithelial cells (EpiC), smooth muscle cells (SMC), pulmonary fibroblasts (PF) and pulmonary endothelial cells (PEC).

CREDIT: Hubert Tseng/Rice University

http://news.rice.edu/wp-content/uploads/2013/01/0128-LUNG-petri-lg.jpg

CAPTION: Rice University spinoff company Nano3D Biosciences uses magnetic levitation to grow three dimensional cell cultures. The technology uses inert, nontoxic nanoparticles and magnets to lift and suspend cells as they grow and divide.

CREDIT: Nano3D Biosciences

http://news.rice.edu/wp-content/uploads/2013/01/0128-LUNG-group-lg.jpg

CAPTION: Scientists from Rice University and the Rice spinoff company Nano3D Biosciences have used magnetic levitation to grow realistic lung tissue in vitro. From left are Glauco Souza, Jacob Gage, Tom Killian, Jane Grande-Allen and Hubert Tseng.

CREDIT: Jeff Fitlow/Rice University

A copy of the paper is available at: http://online.liebertpub.com/doi/abs/10.1089/ten.TEC.2012.0157

This release can be found online at news.rice.edu.

Follow Rice News and Media Relations via Twitter @RiceUNews

Located on a 300-acre forested campus in Houston, Rice University is consistently ranked among the nation's top 20 universities by U.S. News & World Report. Rice has highly respected schools of Architecture, Business, Continuing Studies, Engineering, Humanities, Music, Natural Sciences and Social Sciences and is home to the Baker Institute for Public Policy. With 3,708 undergraduates and 2,374 graduate students, Rice's undergraduate student-to-faculty ratio is 6-to-1. Its residential college system builds close-knit communities and lifelong friendships, just one reason why Rice has been ranked No. 1 for best quality of life multiple times by the Princeton Review and No. 2 for "best value" among private universities by Kiplinger's Personal Finance. To read "What they're saying about Rice," go to http://tinyurl.com/AboutRice.

David Ruth | EurekAlert!
Further information:
http://www.rice.edu

More articles from Medical Engineering:

nachricht Surgery involving ultrasound energy found to treat high blood pressure
24.05.2018 | Queen Mary University of London

nachricht XXL computed tomography: a new dimension in X-ray analysis
17.05.2018 | Fraunhofer-Gesellschaft

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Molecular switch will facilitate the development of pioneering electro-optical devices

A research team led by physicists at the Technical University of Munich (TUM) has developed molecular nanoswitches that can be toggled between two structurally different states using an applied voltage. They can serve as the basis for a pioneering class of devices that could replace silicon-based components with organic molecules.

The development of new electronic technologies drives the incessant reduction of functional component sizes. In the context of an international collaborative...

Im Focus: LZH showcases laser material processing of tomorrow at the LASYS 2018

At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.

At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...

Im Focus: Self-illuminating pixels for a new display generation

There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?

At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...

Im Focus: Explanation for puzzling quantum oscillations has been found

So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics

Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...

Im Focus: Dozens of binaries from Milky Way's globular clusters could be detectable by LISA

Next-generation gravitational wave detector in space will complement LIGO on Earth

The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Save the date: Forum European Neuroscience – 07-11 July 2018 in Berlin, Germany

02.05.2018 | Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

 
Latest News

When corals eat plastics

24.05.2018 | Ecology, The Environment and Conservation

Surgery involving ultrasound energy found to treat high blood pressure

24.05.2018 | Medical Engineering

First chip-scale broadband optical system that can sense molecules in the mid-IR

24.05.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>