Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Innovative Tomographic Imaging Process

04.04.2013
Scanning Laser Optical Tomography (SLOT) is a fast method which can be used for imaging biological tissue or complete organs in a high 3-D resolution.

The Laser Zentrum Hannover e.V. (LZH) has filed a patent application for a SLOT process, which was originally developed as a 3-D fluorescence process for quickly scanning larger samples. SLOT, which can be viewed as the optical equivalent to computer tomography, works with isotropic resolution, that is, with the same resolution in all three spatial planes.


Data of a murine lung. Left: Transmission image (photo-diode). Middle: Autofluorescence image (photo multiplier tube). Right: Superposition of both signals (red: PD, green: PMT). (Scale 500 µm)

It simultaneously records transmissive, scattered and fluorescent light. Samples can thus be imaged with a 3-D resolution of at least 1/1000 of the object size, in a short time. The goal of the current project is further development of this promising technology, so that an imaging speed of 20 seconds for 600 individual projections can be reached.

SLOT has several advantages over optical projection tomography: Apart from homogeneous lighting with a 300 times higher photon exploitation and a high signal-to-noise ratio of 10-90 dB, ring artefacts and speckles can be avoided, due to one-dimensional detection. Furthermore, the process can be also be used with intrinsic (absorption, scattering, auto-fluorescence) and extrinsic (fluorescence and absorption marker) contrast mechanisms.

Based on intrinsic contrast processes, high resolution, ex vivo volumetric images could be made, for example of locust heads and mouse lungs. By using absorption and auto-fluorescent imaging, the researchers at the LZH could image lung structures down to the resolution of a single alveola.

Further, SLOT can be used to determine objects on non-transparent sample surfaces. In 2012, the three-dimensional visualization of bacteria growth on the surface of dental implants was possible, including images of the in vitro development of the micro-organisms. As fluorescent staining was not necessary, these images were given a fourth dimension.

The technical basis for this process uses detection of the scattered laser light from living bio-films, or correspondingly the wavelength-dependent absorption of metabolism markers such as 2,3,5- Triphenyltetrazoliumchloride (TTC), which are implemented and enriched in the metabolically active bacteria cells in 1,3,5 Triphenylformazan (TPF).

Currently, various investigations on establishing this new imaging process are being carried out in close cooperation with the University of Veterinary Medicine Hannover and the Hannover Medical School. Scientists in the Biophotonic Imaging and Manipulation Group at the LZH are working together with industrial partners to establish SLOT as a fully-automated tomographic module, the so-called Laser-Scanning Tomographic Module (LSTM)., Built into an existing confocal and 2-photon microscope, this unit could be used to monitor the fluctuation of NAD/NADH, cAMP or calcium ions, or for the analysis of the intra- and extracellular accumulation of micro- and nanoparticles in cell aggregates. LSTM is also a promising, innovative technology for non-invasive, temporally and spatially resolved investigations on artificial tissue, which is subsequently subject to in vitro investigations.

The development of LSTM is financially supported by the German Federal Ministry of Economics and Technology (BMWi) within the Framework of the Central Innovation Program SME (ZIM). Besides the LZH, project partners are the companies LaVision BioTec GmbH and Scivis wissenschaftliche Bildverarbeitung GmbH.

Contact:
Laser Zentrum Hannover e.V.
Michael Botts
Hollerithallee 8
D-30419 Hannover, Germany
Tel.: +49 511 2788-151
Fax: +49 511 2788-100
E-Mail: m.botts@lzh.de

The Laser Zentrum Hannover e.V. (LZH) carries out research and development in the field of laser technology and is supported by the Ministry of Economic Affairs, Labour and Transport of the State of Lower Saxony (Niedersächsisches Ministerium für Wirtschaft, Arbeit und Verkehr).

Michael Botts | Laser Zentrum Hannover e.V.
Further information:
http://www.lzh.de

More articles from Medical Engineering:

nachricht 3-D visualization of the pancreas -- new tool in diabetes research
15.03.2017 | Umea University

nachricht New PET radiotracer identifies inflammation in life-threatening atherosclerosis
02.03.2017 | Society of Nuclear Medicine

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>