Routine screenings for breast, colon and lung cancers have improved treatment and outcomes for patients with these diseases, largely because the cancer can be detected early. But because little is known about how pancreatic cancer behaves, patients often receive a diagnosis when it’s already too late.
U of Washington
This prototype of a microfluidic device has both curved and straight channels for transporting tissue biopsies. The silicon material is lightweight, flexible and transparent.
University of Washington scientists and engineers are developing a low-cost device that could help pathologists diagnose pancreatic cancer earlier and faster. The prototype can perform the basic steps for processing a biopsy, relying on fluid transport instead of human hands to process the tissue. The team presented its initial results this month (February 2014) at the SPIE Photonics West conference and recently filed a patent for this first-generation device and future technology advancements.
“This new process is expected to help the pathologist make a more rapid diagnosis and be able to determine more accurately how invasive the cancer has become, leading to improved prognosis,” said Eric Seibel, a UW research professor of mechanical engineering and director of the department’s Human Photonics Laboratory.
The new instrumentation would essentially automate and streamline the manual, time-consuming process a pathology lab goes through to diagnose cancer. Currently, a pathologist takes a biopsy tissue sample, then sends it to the lab where it’s cut into thin slices, stained and put on slides, then analyzed optically in 2-D for abnormalities.
The UW’s technology would process and analyze whole tissue biopsies for 3-D imaging, which offers a more complete picture of the cellular makeup of a tumor, said Ronnie Das, a UW postdoctoral researcher in bioengineering who is the lead author on a related paper.
“As soon as you cut a piece of tissue, you lose information about it. If you can keep the original tissue biopsy intact, you can see the whole story of abnormal cell growth. You can also see connections, cell morphology and structure as it looks in the body,” Das said.
The research team is building a thick, credit card-sized, flexible device out of silicon that allows a piece of tissue to pass through tiny channels and undergo a series of steps that replicate what happens on a much larger scale in a pathology lab. The device harnesses the properties of microfluidics, which allows tissue to move and stop with ease through small channels without needing to apply a lot of external force. It also keeps clinicians from having to handle the tissue; instead, a tissue biopsy taken with a syringe needle could be deposited directly into the device to begin processing.
Researchers say this is the first time material larger than a single-celled organism has successfully moved in a microfluidic device. This could have implications across the sciences in automating analyses that usually are done by humans.
Das and Chris Burfeind, a UW undergraduate student in mechanical engineering, designed the device to be simple to manufacture and use. They first built a mold using a petri dish and Teflon tubes, then poured a viscous, silicon material into the mold. The result is a small, transparent instrument with seamless channels that are both curved and straight.
The researchers have used the instrument to process a tissue biopsy one step at a time, following the same steps as a pathology lab would. Next, they hope to combine all of the steps into a more robust device – including 3-D imaging – then build and optimize it for use in a lab. Future iterations of the device could include layers of channels that would allow more analyses on a piece of tissue without adding more bulk to the device.
For Burfeind, who started working in Seibel’s lab his sophomore year, the research apprenticeship has been beneficial both for his college experience and future career, and for the lab.
“I’m getting theory from my professors in class, then applying it to my research here,” Burfeind said. “I see this research as a way to enhance cancer diagnosis and catch it earlier so patients can have a better chance of survival.”
The UW researchers say the technology could be used overseas as an over-the-counter kit that would process biopsies, then send that information to pathologists who could look for signs of cancer from remote locations. Additionally, it could potentially reduce the time it takes to diagnose cancer to a matter of minutes, Das said.
The team is working with Melissa Upton, a pathologist with UW Medicine. The research is funded by the National Science Foundation Bioengineering division and the U.S. Department of Education Graduate Assistance in Areas of National Need program.
For more information, contact Seibel at firstname.lastname@example.org or 206-616-1486, and Das at email@example.com or 206-221-3813.
Grant numbers: NSF Bioengineering division (CBET-1212540).
Michelle Ma | EurekAlert!
Siemens Healthcare presents syngo.via RT Image Suite software solution
24.04.2015 | Siemens AG
Vanderbilt Team First to Blend High-End Imaging Techniques
17.03.2015 | Vanderbilt University Medical Center
KAIST researchers published an article on the development of a novel technique to precisely track the 3-D positions of optically-trapped particles having complicated geometry in high speed in the April 2015 issue of Optica.
Daejeon, Republic of Korea, April 23, 2015--Optical tweezers have been used as an invaluable tool for exerting micro-scale force on microscopic particles and...
A very small and rare species of shark is swimming its way through scientific literature. But don't worry, the chances of this inches-long vertebrate biting...
Ever since computers have been small enough to be fixtures on desks and laps, their central processing has functioned something like an atomic Etch A Sketch, with electromagnetic fields pushing data bits into place to encode data.
Unfortunately, the same drawbacks and perils of the mechanical sketch board have been just as pervasive in computing: making a change often requires starting...
How is lightning initiated in thunderclouds? This is difficult to answer - how do you measure electric fields inside large, dangerously charged clouds? It was discovered, more or less by coincidence, that cosmic rays provide suitable probes to measure electric fields within thunderclouds. This surprising finding is published in Physical Review Letters on April 24th. The measurements were performed with the LOFAR radio telescope located in the Netherlands.
How is lightning initiated in thunderclouds? This is difficult to answer - how do you measure electric fields inside large, dangerously charged clouds? It was...
Max Planck researcher Buhalqem Mamtimin determines how much nitrogen oxide is released into the atmosphere from agriculturally used oases.
In order to make statements about current and future air pollution, scientists use models which simulate the Earth’s atmosphere. A lot of information such as...
23.04.2015 | Event News
23.04.2015 | Event News
13.04.2015 | Event News
27.04.2015 | Life Sciences
27.04.2015 | Power and Electrical Engineering
27.04.2015 | Life Sciences