Researchers are leveraging Ohio Supercomputer Center resources to develop computer-assisted diagnosis tools that will provide pathologists grading Follicular Lymphoma samples with quicker, more consistently accurate diagnoses.
“The advent of digital whole-slide scanners in recent years has spurred a revolution in imaging technology for histopathology,” according to Metin N. Gurcan, Ph.D., an associate professor of Biomedical Informatics at The Ohio State University Medical Center. “The large multi-gigapixel images produced by these scanners contain a wealth of information potentially useful for computer-assisted disease diagnosis, grading and prognosis.”
Follicular Lymphoma (FL) is one of the most common forms of non-Hodgkin Lymphoma occurring in the United States. FL is a cancer of the human lymph system that usually spreads into the blood, bone marrow and, eventually, internal organs.
A World Health Organization pathological grading system is applied to biopsy samples; doctors usually avoid prescribing severe therapies for lower grades, while they usually recommend radiation and chemotherapy regimens for more aggressive grades.
Accurate grading of the pathological samples generally leads to a promising prognosis, but diagnosis depends solely upon a labor-intensive process that can be affected by human factors such as fatigue, reader variation and bias. Pathologists must visually examine and grade the specimens through high-powered microscopes.
Processing and analysis of such high-resolution images, Gurcan points out, remain non-trivial tasks, not just because of the sheer size of the images, but also due to complexities of underlying factors involving differences in staining, illumination, instrumentation and goals.
To overcome many of these obstacles to automation, Gurcan and medical center colleagues, Dr. Gerard Lozanski and Dr. Arwa Shana’ah, turned to the Ohio Supercomputer Center.
Ashok Krishnamurthy, Ph.D., interim co-executive director of the center, and Siddharth Samsi, a computational science researcher there and an OSU graduate student in Electrical and Computer Engineering, put the power of a supercomputer behind the process.
“Our group has been developing tools for grading of follicular lymphoma with promising results,” said Samsi. “We developed a new automated method for detecting lymph follicles using stained tissue by analyzing the morphological and textural features of the images, mimicking the process that a human expert might use to identify follicle regions. Using these results, we developed models to describe tissue histology for classification of FL grades.”
Histological grading of FL is based on the number of large malignant cells counted in within tissue samples measuring just 0.159 square millimeters and taken from ten different locations. Based on these findings, FL is assigned to one of three increasing grades of malignancy: Grade I (0-5 cells), Grade II (6-15 cells) and Grade III (more than 15 cells).
“The first step involves identifying potentially malignant regions by combining color and texture features,” Samsi explained. “The second step applies an iterative watershed algorithm to separate merged regions and the final step involves eliminating false positives.”
The large data sizes and complexity of the algorithms led Gurcan and Samsi to leverage the parallel computing resources of OSC’s Glenn Cluster in order to reduce the time required to process the images. They used MATLAB® and the Parallel Computing Toolbox™ to achieve significant speed-ups. Speed is the goal of the National Cancer Institute-funded research project, but accuracy is essential. Gurcan and Samsi compared their computer segmentation results with manual segmentation and found an average similarity score of 87.11 percent.
“This algorithm is the first crucial step in a computer-aided grading system for Follicular Lymphoma,” Gurcan said. “By identifying all the follicles in a digitized image, we can use the entire tissue section for grading of the disease, thus providing experts with another tool that can help improve the accuracy and speed of the diagnosis.”Contact:
Jamie Abel | Ohio State University
Novel PET imaging agent could help guide therapy for brain diseases
03.04.2018 | Society of Nuclear Medicine and Molecular Imaging
New Computer Architecture: Time Lapse for Dementia Research
29.03.2018 | Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE)
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
20.04.2018 | Physics and Astronomy
20.04.2018 | Interdisciplinary Research
20.04.2018 | Physics and Astronomy