Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Chips that listen to bacteria

11.02.2014
CMOS technology provides new insights into how biofilms form

In a study published today in Nature Communications, a research team led by Ken Shepard, professor of electrical engineering and biomedical engineering at Columbia Engineering, and Lars Dietrich, assistant professor of biological sciences at Columbia University, has demonstrated that integrated circuit technology, the basis of modern computers and communications devices, can be used for a most unusual application—the study of signaling in bacterial colonies.


The development of colony biofilms by Pseudomonas aeruginosa is affected by redox-active compounds called phenazines. A phenazine-null mutant forms a hyperwrinkled colony with prominent spokes, while wild-type colonies are more constrained and smooth.

Credit: Hassan Sakhtah, Columbia University

They have developed a chip based on complementary metal-oxide-semiconductor (CMOS) technology that enables them to electrochemically image the signaling molecules from these colonies spatially and temporally. In effect, they have developed chips that "listen" to bacteria.

"This is an exciting new application for CMOS technology that will provide new insights into how biofilms form," says Shepard. "Disrupting biofilm formation has important implications in public health in reducing infection rates."

The researchers, who include PhD students Dan Bellin (electrical engineering) and Hassan Sakhtah (biology), say that this is the first time integrated circuits have been used for such an application—imaging small molecules electrochemically in a multicellular structure. While optical microscopy techniques remain paramount for studying biological systems (using photons allows for relatively non-invasive interaction to the biological system being studied), they cannot directly detect critical components of physiology, such as primary metabolism and signaling factors.

The team thought there might be a better way to directly detect small molecules through techniques that employ direct transduction to electrons, without using photos as an intermediary. They made an integrated circuit, a chip that, Shepard says, is an "'active' glass slide, a slide that not only forms a solid-support for the bacterial colony but also 'listens' to the bacteria as they talk to each other."

Cells, Dietrich explains, mediate their physiological activities using secreted molecules. The team looked specifically at phenazines, which are secreted metabolites that control gene expression. Their study found that the bacterial colonies produced a phenazine gradient that, they say, is likely to be of physiological significance and contribute to colony morphogenesis.

"This is a big step forward," Dietrich continues. "We describe using this chip to 'listen in' on conversations taking place in biofilms, but we are also proposing to use it to interrupt these conversations and thereby disrupt the biofilm. In addition to the pure science implications of these studies, a potential application of this would be to integrate such chips into medical devices that are common sites of biofilm formation, such as catheters, and then use the chips to limit bacterial colonization."

The next step for the team will be to develop a larger chip that will enable larger colonies to be imaged at higher spatial and temporal resolutions.

"This represents a new and exciting way in which solid-state electronics can be used to study biological systems," Shepard adds. "This is one of the many emerging ways integrated circuit technology is having impact in biotechnology and the life sciences."

The study was supported by the National Institutes of Health and the National Science Foundation.

Holly Evarts | EurekAlert!
Further information:
http://www.columbia.edu

More articles from Medical Engineering:

nachricht Novel PET imaging agent could help guide therapy for brain diseases
03.04.2018 | Society of Nuclear Medicine and Molecular Imaging

nachricht New Computer Architecture: Time Lapse for Dementia Research
29.03.2018 | Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE)

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Molecules Brilliantly Illuminated

Physicists at the Laboratory for Attosecond Physics, which is jointly run by Ludwig-Maximilians-Universität and the Max Planck Institute of Quantum Optics, have developed a high-power laser system that generates ultrashort pulses of light covering a large share of the mid-infrared spectrum. The researchers envisage a wide range of applications for the technology – in the early diagnosis of cancer, for instance.

Molecules are the building blocks of life. Like all other organisms, we are made of them. They control our biorhythm, and they can also reflect our state of...

Im Focus: Spider silk key to new bone-fixing composite

University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.

Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Structured light and nanomaterials open new ways to tailor light at the nanoscale

23.04.2018 | Physics and Astronomy

On the shape of the 'petal' for the dissipation curve

23.04.2018 | Physics and Astronomy

Clean and Efficient – Fraunhofer ISE Presents Hydrogen Technologies at the HANNOVER MESSE 2018

23.04.2018 | Trade Fair News

VideoLinks
Science & Research
Overview of more VideoLinks >>>