Microvesicles are smallest cell elements which are present in all body fluids and are different, depending on whether a person is healthy or sick. This could contribute to detecting numerous diseases, such as, e.g., carcinomas, at an early stage, and to treating them more efficiently.
The problem is that the diameter of the relevant microvesicles generally lies below 100 nm, which makes them technically detectable, but their exact size and concentration hardly possible to determine. A new device is now to provide the metrological basis for these promising biomarkers.
The vacuum-compatible version of the Pilatus hybrid pixel detector for X-rays, which was developed by Dectris in cooperation with the Physikalisch-Technische Bundesanstalt (PTB), now allows also the size of nano-particles - which, to date, have been difficult to characterize - to be determined using small-angle X-ray scattering at low photon energies. The detector can also be used for other X-ray-based techniques.
What makes this detector unique is the size of its total surface (17 cm × 18 cm) as well as the fact that it can be operated in vacuum. Operating the detector in vacuum drastically increases the sensitivity of the measuring facility, since the soft X-rays, which are scattered on the sample, are not absorbed by air molecules on their way towards the detector.
This device now allows, for example, experiments for size determination of nanoparticles to be carried out with small-angle X-ray scattering (SAXS) also at the absorption edges of the light elements calcium, sulphur, phosphor or silicon at photon energies below 5 keV with high dynamics and good spatial resolution.
For a few months, the new Pilatus X-ray detector has been used for some of PTB's own research projects. At the synchrotron radiation source BESSY II in Berlin-Adlershof, where PTB has been operating its own laboratory for 15 years, scientists are now using the new detector, for example, to establish the - urgently needed - metrological basis for the size determination of microvesicles.
A project carried out within the scope of the European Metrology Research Programme (EMRP) and with the significant participation of the Amsterdam Medical Center in the Netherlands is to contribute decisively to fully exploiting the potential of microvesicles for the early diagnosis of diseases
For further information about the detector, please visit:
For further information about the characterization of microvesicles, please visit:
PTB Working Group 7.11 "X-ray Radiometry",
phone: +49 (0)30 3481-7110,
(untill 3. December contact is only possible by e-mail)
Michael Krumrey | Source: EurekAlert!
Further information: www.ptb.de
More articles from Life Sciences:
Spheres can form squares
24.05.2013 | Wageningen University
Ferrets, pigs susceptible to H7N9 avian influenza virus
24.05.2013 | NIH/National Institute of Allergy and Infectious Diseases
This morning at 05:45 CEST, the earth trembled beneath the Okhotsk Sea in the Pacific Northwest. The quake, with a magnitude of 8.2, took place at an exceptional depth of 605 kilometers.
Because of the great depth of the earthquake a tsunami is not expected and there should also be no major damage due to shaking.
Professor Frederik Tilmann of the GFZ German Research Centre for Geosciences: "The epicenter is exceptionally deep, far below the earth's crust in the mantle. Such strong ...
The Ring Nebula's distinctive shape makes it a popular illustration for astronomy books. But new observations by NASA's Hubble Space Telescope of the glowing gas shroud around an old, dying, sun-like star reveal a new twist.
"The nebula is not like a bagel, but rather, it's like a jelly doughnut, because it's filled with material in the middle," said C. Robert O'Dell of Vanderbilt University in Nashville, Tenn.
He leads a research team that used Hubble and several ground-based telescopes to obtain the best view yet of ...
New indicator molecules visualise the activation of auto-aggressive T cells in the body as never before
Biological processes are generally based on events at the molecular and cellular level. To understand what happens in the course of infections, diseases or normal bodily functions, scientists would need to examine individual cells and their activity directly in the tissue.
The development of new microscopes and fluorescent dyes in ...
A fried breakfast food popular in Spain provided the inspiration for the development of doughnut-shaped droplets that may provide scientists with a new approach for studying fundamental issues in physics, mathematics and materials.
The doughnut-shaped droplets, a shape known as toroidal, are formed from two dissimilar liquids using a simple rotating stage and an injection needle. About a millimeter in overall size, the droplets are produced individually, their shapes maintained by a surrounding springy material made of polymers.
Droplets in this toroidal shape made ...
Frauhofer FEP will present a novel roll-to-roll manufacturing process for high-barriers and functional films for flexible displays at the SID DisplayWeek 2013 in Vancouver – the International showcase for the Display Industry.
Displays that are flexible and paper thin at the same time?! What might still seem like science fiction will be a major topic at the SID Display Week 2013 that currently takes place in Vancouver in Canada.
High manufacturing cost and a short lifetime are still a major obstacle on ...
24.05.2013 | Life Sciences
24.05.2013 | Ecology, The Environment and Conservation
24.05.2013 | Physics and Astronomy
17.05.2013 | Event News
15.05.2013 | Event News
08.05.2013 | Event News