Anthrax uses a receptor on the surface of cells to inject its lethal toxins. However, the physiological function of this receptor, named Anthrax Toxin Receptor 2a (Antxr2a), remained unknown until now. A team led by Marcos Gonzalez-Gaitan, a professor at the University of Geneva (UNIGE), Switzerland, in collaboration with Gisou van der Goot at EPFL (École Polytechnique Fédérale de Lausanne), reveals that Antxr2a actually plays a role in embryonic development, orienting cell division along a specific plane, which is a prelude to the formation of future tissues and organs.
At the cellular level, this receptor exerts traction on the system that allows chromosomes to separate to opposite poles, the mitotic spindle, to position it along the plane of division. These results are presented in the journal Nature Cell Biology.
Anthrax is a particularly virulent germ once a person is infected by inhaling its spores. The severity of symptoms, which affect various organs, is mainly due to bacterial toxins which are lethal to cells. It is by attempting to understand how the bacillus' toxins enter cells that the Antxr2a receptor was discovered. Otherwise, its physiological role would not have been identified at present.
A cap of proteins indicates the plane of division
During animal development, the orientation of cell division along a specific plane is important for the organization of the different tissues and the generation of cellular diversity. This orientation is provided by the position of the mitotic spindle in the cell that is about to divide. This temporary assembly of microtubules forms an actual spindle between opposite poles of the cell in order to guide the migration of each set of chromosomes.
"When the cell receives an external signal to initiate its division, a cascade of biochemical events is launched to transmit the message to the interior of the cell and have it carried out. We knew that an external signal, a protein called Wnt, was necessary to properly position the mitotic spindle, but knew nothing of the intracellular messengers involved," explains Marcos Gonzalez-Gaitan, Professor in the Departments of Biochemistry and Molecular Biology at the University of Geneva.
This has now been accomplished. The scientist and his group have established the complete sequence of intracellular events allowing the mitotic spindle to align itself along the general plane of division. They conducted their experiments on zebrafish embryos, a model system in developmental studies. "Once Wnt binds to the cell membrane, different molecular agents prompt the formation of a layer of filamentous proteins along the cell membrane, at the site of the future plane of division," explains Irinka Castanon, first author of the article.
The anthrax receptor used as a control lever
This internal 'cap' associates itself in turn with the Antxr2a receptors, known to bind the anthrax toxin. The accumulation of these receptors will thus form a second layer, superimposed on the first. Everything is now in place for the final phase: "The Antxr2a receptors recruit in turn 'motor' proteins capable of attaching themselves to the mitotic spindle and pulling it towards the internal cap," states Marcos Gonzalez-Gaitan. Motor proteins probably act by travelling back up along the cap's filaments, allowing the alignment of the spindle with the plane of cell division.
In mammals, the Antxr2a receptor is also involved in the formation and proliferation of blood vessels. "It is therefore possible that the role of this receptor in the orientation of cell division is not restricted solely to embryonic development," states the professor, who is a member of two Swiss National Research Programs: Frontiers in Genetics, and Chemical Biology.
Marcos Gonzalez-Gaitan | Source: EurekAlert!
Further information: www.unige.ch
More articles from Life Sciences:
New way to improve antibiotic production
18.06.2013 | Norwich BioScience Institutes
Missing enzyme linked to drug addiction
18.06.2013 | The Endocrine Society
... two engines aircraft project “Elektro E6”.
The countdown has been started for opening the gates again for the worldwide leading aviation and space event in Le Bourget, Paris from June 17th - 23rd, 2013.
EADCO & PC-Aero will present at the Paris Air Show in Hall H4 booth F-7 their new future aircraft and innovative project: ...
Siemens scientists have developed new kinds of ceramics in which they can embed transformers.
The new development allows power supply transformers to be reduced to one fifth of their current size so that the normally separate switched-mode power supply units of light-emitting diodes can be integrated into the module's heat sink.
The new technology was developed in cooperation with industrial and research partners who ...
Cheaper clean-energy technologies could be made possible thanks to a new discovery.
Led by Raymond Schaak, a professor of chemistry at Penn State University, research team members have found that an important chemical reaction that generates hydrogen from water is effectively triggered -- or catalyzed -- by a nanoparticle composed of nickel and phosphorus, two inexpensive elements that are abundant on Earth. ...
The Fraunhofer Institute for Laser Technology ILT generated a lot of interest at the LASER World of Photonics 2013 trade fair with its numerous industrial laser technology innovations.
Its highlights included beam sources and manufacturing processes for ultrashort laser pulses as well as ways to systematically optimize machining processes using computer simulations. There was even a specialist booth at the fair dedicated to the revolutionary technological potential of digital photonic production.
Now in its fortieth year, LASER World ...
It's not reruns of "The Jetsons", but researchers working at the National Institute of Standards and Technology (NIST) have developed a new microscopy technique that uses a process similar to how an old tube television produces a picture—cathodoluminescence—to image nanoscale features.
Combining the best features of optical and scanning electron microscopy, the fast, versatile, and high-resolution technique allows scientists to view surface and subsurface features potentially as small as 10 nanometers in size.
The new microscopy technique, described in the journal AIP Advances,* uses a beam of electrons to excite a specially ...
18.06.2013 | Materials Sciences
18.06.2013 | Health and Medicine
18.06.2013 | Life Sciences
14.06.2013 | Event News
13.06.2013 | Event News
10.06.2013 | Event News