Consuming putrid food can be lethal as it allows bacterial pathogens to enter the digestive system. To detect signs of decay and thus allowing us and other animals to avoid such food poisoning is one of the main tasks of the sense of smell.
Behavioral scientists and neurobiologists at the Max Planck Institute for Chemical Ecology in Jena, Germany, have now for the first time decoded the neural mechanisms underlying an escape reflex in fruit flies (Drosophila) activated in order to avoid eating and laying eggs in food infected by toxic microorganisms. A super-sensitive and completely dedicated neural line, from olfactory receptor, via sensory neuron and primary brain neurons, is activated as soon as the tiniest amount of geosmin is in the air. Geosmin is a substance released by bacteria and mold fungi toxic to the fly.
This stimulus overrides all other food odor signals, irrespective of how attractive they are on their own. Consequently, geosmin is a full STOP signal that prevents flies from eating and laying eggs in toxic food, similar to when we open the fridge and smell last week’s forgotten dinner. (CELL, December 7, 2012, DOI: 10.1016/j.cell.2012.09.046)
Healthy and unhealthy microbes
The shocking number of fatalities in Germany in spring 2011 linked to fenugreek sprouts contaminated with EHEC bacteria showed that it is crucial for every foraging organism to be able to distinguish between fresh food and that infested by pathogenic microbes. Food, whether meat or vegetables, is more or less always colonized by bacteria and other microbes, their numbers varying with differing degrees of freshness and storage conditions. Usually, the immune system can cope with microorganisms in the food. Consequently, if food decay is not too far advanced, animals can digest the food without harm. But what protects us and other animals when the concentration of toxin-producing and often pathogenic microbes has become dangerously high?
Rotten smell as warning signal
In many cases visual signs allow us to avoid putrid food – who wants to bite into a rotting orange anyway? A more direct and common signal, however, is perception of certain odors released by hazardous microbes and/or their activity. If these odors are detected, the food item is directly discarded, a behavior which saves lives. But which neural functions underlie such an avoidance or even flight behavior? What does the way from the odor molecule and the olfactory receptors into the brain and finally to the reaction of the animal look like?
Geosmin and the model Drosophila
The genetically very well characterized fruit or vinegar fly Drosophila melanogaster and related species are perfect study objects to answer such a question. The flies typically feed on yeasts growing on rotting fruit. This is the stage where they must distinguish between “good” and “bad” microbes, i.e. at which stage of decomposition the fruit is. In our experiments it was shown that flies consuming pathogenic bacteria or fungi died very fast, and eggs deposited did not result in viable larvae. The odor substance geosmin is known to be produced by several fungi and bacteria and may trigger deterrent reactions. Geosmin is responsible for a smell quite familiar to us: the strong scent of wet soils, especially after droughts. The human nose is highly sensitive to this odor and can detect it at concentrations as low as 0,1 ppb (parts per billion). Max Planck scientists now found that Drosophila antennae are even more sensitive to geosmin.
“We started with electrophysiological experiments and analyzed all olfactory sensory neurons on the fly antenna successively – more than thousand measurements,” says Marcus Stensmyr, first author of the study. Already at this stage, the first unexpected result became obvious: Only a single neuron type labeled “ab4B” responded to geosmin. These neurons carry the specific receptor Or56a, which we could show reacts exclusively to geosmin. The specificity could be established both in single neuron recordings linked to gas chromatography, where more than 3000 odors were tested, and in experiments using cell cultures ectopically expressing the receptor. Optical imaging of the Drosophila brain revealed another interesting result: From the approximately 50 glomeruli that constitute the antennal lobe, the olfactory center of the flies, only one, labeled DA2, was activated by geosmin. DA2 was found in the same region as other glomeruli that are involved in odor-evoked aversive behavior (see press release “Flies Process Attractive and Deterrent Odors in Different Brain Areas − Newly developed analytic device Flywalk allows accurate studies of insect behavior to be made”, April 25, 2012). Stimulation of DA2 resulted in activation of a single type of a specific projection neuron (PN) conveying the message regarding geosmin presence to higher brain areas. PNs are otherwise often more broadly tuned, providing a cross-glomerulus pattern for olfactory coding. “However, this is different in the case of geosmin, Or56a, DA2 and the related PNs,” says Bill Hansson, leader of the study. In this circuit, the stimulus is directly patched through from antenna to behavior, without any detours. Until now, such patterns have only been measured in the responses to sex pheromones, if that is at all comparable, report the scientists. For the first time, we see a fully dedicated neural line for an odor involved in feeding behavior.
“Flywalk” experiments confirm laboratory measurements
Behavioral assays using the recently established “Flywalk” system confirm the nerve and brain measurements in the laboratory. In the “Flywalk”, single fruit flies are placed into small glass tubes, different odors are applied, and the behavior of the flies is recorded by a camera connected directly to a computer quantifying the behavior.
The geosmin stimulus, mediated through the dedicated neural line, does not only cause the flies to stop or move away from the odor source, it also overrides all simultaneously offered highly attractive odors, such as vinegar or fruit scents, at minute concentrations. This means that flies are programmed to avoid geosmin even in a mixture of different smells and will thus not be tempted to consume pathogens accidentally. This is important, because odor mixtures are the rule and not an exception in a natural environment. The response to geosmin is so strong that it also regulates oviposition behavior in females: female Drosophila laid their fertilized eggs only on substrates overgrown with ordinary yeast; they strictly avoided culture media containing the highly pathogenic geosmin producing bacterium Streptomyces coelicolor. In a Streptomyces mutant deficient in its geosmin-producing enzyme, the flies did, however, oviposit, and the larvae consequently died. Geosmin is thus sufficient and necessary to elicit the avoidance behavior. [BSH/JWK]
Marcus C. Stensmyr, Hany K. M. Dweck, Abu Farhan, Irene Ibba, Antonia Strutz, Latha Mukunda, Jeanine Linz, Kathrin Steck, Sofia Lavista-Llanos, Dieter Wicher, Silke Sachse, Markus Knaden, Paul G. Becher, Yoichi Seki, Bill S. Hansson (2012).
A conserved dedicated olfactory circuit for detecting harmful microbes in Drosophila.
CELL, December 7, DOI: 10.1016/j.cell.2012.09.046
Prof. Dr. Bill S. Hansson, MPI Chemical Ecology, email@example.com, +49 (0)3641 571401
Dr. Marcus C. Stensmyr, MPI Chemical Ecology, firstname.lastname@example.org, +49 (0)3641 571420
Angela Overmeyer M.A., +49 3641 57-2110, email@example.com
Dr. Jan-Wolfhard Kellmann | Source: Max-Planck-Institut
Further information: www.ice.mpg.de
Further Reports about: Brain > brain area > chemical engineering > DA2 > Drosophila melanogaster > Ecology > Food Chain Plus > fruit flies > geosmin > Max Planck Institute > MPI > neural mechanism > pathogenic microbes > sex pheromone > single neuron > Streptomyces
More articles from Life Sciences:
New way to improve antibiotic production
18.06.2013 | Norwich BioScience Institutes
Missing enzyme linked to drug addiction
18.06.2013 | The Endocrine Society
... two engines aircraft project “Elektro E6”.
The countdown has been started for opening the gates again for the worldwide leading aviation and space event in Le Bourget, Paris from June 17th - 23rd, 2013.
EADCO & PC-Aero will present at the Paris Air Show in Hall H4 booth F-7 their new future aircraft and innovative project: ...
Siemens scientists have developed new kinds of ceramics in which they can embed transformers.
The new development allows power supply transformers to be reduced to one fifth of their current size so that the normally separate switched-mode power supply units of light-emitting diodes can be integrated into the module's heat sink.
The new technology was developed in cooperation with industrial and research partners who ...
Cheaper clean-energy technologies could be made possible thanks to a new discovery.
Led by Raymond Schaak, a professor of chemistry at Penn State University, research team members have found that an important chemical reaction that generates hydrogen from water is effectively triggered -- or catalyzed -- by a nanoparticle composed of nickel and phosphorus, two inexpensive elements that are abundant on Earth. ...
The Fraunhofer Institute for Laser Technology ILT generated a lot of interest at the LASER World of Photonics 2013 trade fair with its numerous industrial laser technology innovations.
Its highlights included beam sources and manufacturing processes for ultrashort laser pulses as well as ways to systematically optimize machining processes using computer simulations. There was even a specialist booth at the fair dedicated to the revolutionary technological potential of digital photonic production.
Now in its fortieth year, LASER World ...
It's not reruns of "The Jetsons", but researchers working at the National Institute of Standards and Technology (NIST) have developed a new microscopy technique that uses a process similar to how an old tube television produces a picture—cathodoluminescence—to image nanoscale features.
Combining the best features of optical and scanning electron microscopy, the fast, versatile, and high-resolution technique allows scientists to view surface and subsurface features potentially as small as 10 nanometers in size.
The new microscopy technique, described in the journal AIP Advances,* uses a beam of electrons to excite a specially ...
18.06.2013 | Materials Sciences
18.06.2013 | Health and Medicine
18.06.2013 | Life Sciences
14.06.2013 | Event News
13.06.2013 | Event News
10.06.2013 | Event News