Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

XMRV and related viruses not confirmed in blood of healthy donors or chronic fatigue syndrome patients

23.09.2011
HHS-funded research was part of efforts to determine whether these viruses could affect safety of blood supply

A study supported by the U.S. Department of Health and Human Services could not validate or confirm previous research findings that suggested the presence of one of several viruses in blood samples of people living with chronic fatigue syndrome. The new study also could not find the viruses in blood samples of healthy donors who were previously known to not have the viruses.

The HHS-supported study examined the validity of testing techniques intended to detect the presence of several viruses, xenotropic murine leukemia virus-related virus (XMRV) or related polytropic murine leukemia viruses (P-MLVs). Such follow-up studies are standard in science to determine whether earlier findings are accurate. The new findings suggest earlier results may have resulted from laboratory error, either contamination or false positive test results.

The initial reports of a link between XMRV and chronic fatigue syndrome prompted HHS to investigate how well tests detect XMRV/P-MLVs, and the prevalence and potential transmission of these viruses in the blood supply. If the viruses had been proven to be present in patients with chronic fatigue syndrome or healthy donors, concerns were raised that these viruses could put the blood supply at risk. The new results were published online on Thursday, Sept. 22, 2011 in Science Express.

“The results of this study, along with other recent findings, reassure us that these viruses do not pose a threat to the safety of the nation’s blood supply,” said Susan B. Shurin, M.D., acting director of the National Heart, Lung, and Blood Institute (NHLBI) of the National Institutes of Health. “These data add to the mounting evidence that there is no need to screen blood donors for them at the present time.”

In the new study, blood samples were taken from healthy donors and from 14 patients with chronic fatigue syndrome who had previously tested positive for XMRV or P-MLV, and samples from another person who had tested positive for XMRV but who did not have chronic fatigue syndrome. The study also used blood samples from healthy volunteers whose blood tests previously had shown no signs of XMRV/P-MLV.

The samples were blinded with no indication as to their source, and sent to nine laboratories. The study involved laboratories supported by several HHS agencies along with Abbott Laboratories, Abbott Park, Ill., GenProbe Inc., San Diego, and the Whittemore Peterson Institute (WPI), Reno, Nev.

The nine laboratories tested identical sets of the new blood samples for the XMRV/P-MLV nucleic acid, for replication of the virus (whether it reproduced itself in cells), and for antibodies to the viruses. Two labs, which previously had reported the association of XMRV with chronic fatigue syndrome, reported the presence of XMRV for some samples. However, the labs reported similar rates of finding XMRV in samples from patients with chronic fatigue syndrome and from healthy donors who were known to not have XMRV. Additional tests run in the same and other laboratories on the same samples did not find XMRV, strongly suggesting that these persons were negative for XMRV/ P-MLVs. This would be a sign that the few observed positive results represented false positives — that is, the results indicated the condition was present when it actually was not.

To investigate the validity of testing techniques and to determine XMRV’s potential impact on the blood supply, HHS formed the Blood XMRV Scientific Research Working Group in December 2009. The NHLBI leads the working group, which includes other HHS agencies including the Office of the Assistant Secretary for Health, NIH’s National Cancer Institute (NCI) and the NIH Clinical Center, as well as the Centers for Disease Control and Prevention and the Food and Drug Administration. The current study is based on the working group’s efforts to determine the best way to test blood samples for the virus.

Researchers used 11 nucleic acid, five antibody, and three culture assays to determine the assays’ abilities to detect XMRV/P-MLVs. The study also checked for evidence of contamination with mouse DNA, because XMRV or its predecessors may be present in some mouse strains and cell lines.

In 2009, WPI researchers had reported a possible link between XMRV and chronic fatigue syndrome. In June 2010, the AABB, a nonprofit U.S. blood banking association, recommended that collection centers discourage people with chronic fatigue syndrome from donating blood.

However, within the last year, evidence emerged suggesting that contamination in the laboratory was potentially responsible for detection of XMRV in some blood samples from patients with chronic fatigue syndrome. To ensure that this did not compromise results in the new study, extensive efforts were undertaken to avoid laboratory contamination and contamination of samples.

The Blood Systems Research Institute, San Francisco, including Graham Simmons, Ph. D., the paper’s lead author, compiled the blood samples and distributed them to participating laboratories. The following labs and investigators were responsible for testing the sample panels:

Abbott (Two labs: Molecular Laboratory and Diagnostic Laboratory) — John Hackett Jr., Ning Tang
CDC — William M. Switzer, Walid Heneine
FDA — Indira K. Hewlett, Jiangqin Zhao
FDA — Shyh-Ching Lo/ Harvey Alter, NIH Clinical Center, Bethesda, Md.
Gen-Probe, Inc. — Jeffrey M. Linnen, Kui Gao
NCI — Mary F. Kearney/John M. Coffin, Tufts University, Boston
NCI — Francis W. Ruscetti
WPI — Judy A. Mikovits, Max A. Pfost
W. Ian Lipkin, M.D., of Columbia University’s Mailman School of Public Health, is currently leading a separate study that is testing fresh blood samples from 150 people living with chronic fatigue syndrome and 150 similar but healthy people. Through this effort, organizers will process, blind, and send samples to laboratories at the FDA, the CDC, and the WPI for testing for the presence of XMRV, MLV or related viruses.

If one of the participating laboratories finds that a sample is virus positive, further tests will be conducted to determine the validity of the result. If one participating laboratory finds a positive sample but another laboratory does not, the same samples can be shipped again, with a new blinded code, to be retested. It is hoped that the results from this study will be available by the end of 2011.

For more information or to schedule an interview with an NHLBI expert, contact the NHLBI Office of Communications at 301-496-4236 or nhlbi_news@nhlbi.nih.gov. To interview an NCI expert, contact NCI Office of Media Relations at 301-496-6641 or ncipressofficers@mail.nih.gov.

Part of the National Institutes of Health, the National Heart, Lung, and Blood Institute (NHLBI) plans, conducts, and supports research related to the causes, prevention, diagnosis, and treatment of heart, blood vessel, lung, and blood diseases; and sleep disorders. The Institute also administers national health education campaigns on women and heart disease, healthy weight for children, and other topics. NHLBI press releases and other materials are available online at www.nhlbi.nih.gov.

NCI leads the National Cancer Program and the NIH effort to dramatically reduce the burden of cancer and improve the lives of cancer patients and their families, through research into prevention and cancer biology, the development of new interventions, and the training and mentoring of new researchers. For more information about cancer, please visit the NCI Web site at www.cancer.gov or call NCI's Cancer Information Service at 1-800-4-CANCER (1-800-422-6237).

About the National Institutes of Health (NIH): NIH, the nation's medical research agency, includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. NIH is the primary federal agency conducting and supporting basic, clinical, and translational medical research, and is investigating the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit www.nih.gov.

NHLBI Communications Office | EurekAlert!
Further information:
http://www.nih.gov

More articles from Life Sciences:

nachricht Study shines light on brain cells that coordinate movement
26.06.2017 | University of Washington Health Sciences/UW Medicine

nachricht New insight into a central biological dogma on ion transport
26.06.2017 | Aarhus University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Study shines light on brain cells that coordinate movement

26.06.2017 | Life Sciences

Smooth propagation of spin waves using gold

26.06.2017 | Physics and Astronomy

Switchable DNA mini-machines store information

26.06.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>