Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Visualizing RNAi at work

08.07.2015

How does single "RISC" accurately cleave and release target RNAs?

University of Tokyo and Kyoto University researchers have revealed the molecular mechanism of RNA interference (RNAi), the phenomenon by which the synthesis of a specific protein is inhibited, by real time observation of target RNA cleavage at the single-molecule level.


Shedding light on the single target cleavage reaction in RNAi. The target RNA cleavage reaction by the RNAi effector complex RISC was monitored in real time at the single-molecule level by using a special microscope. This illustration represents this process. © 2015 Hachiro Hongo and Hisashi Tadakuma.

The phenomenon of RNAi is expected to find applications in medical treatments. RNAi is mediated by RNA-induced silencing complex (RISC), which contains a small RNA and an Argonaute protein at its core and cleaves the target RNA.

However, there were no suitable tools to directly monitor the RNAi reaction and its molecular mechanism by which RISC cleaves the target RNA has remained unclear.

Now, a research group at the University of Tokyo (Professor Takuya Ueda, Professor Yukihide Tomari, Researcher Chunyan Yao and Research Associate Hiroshi M Sasaki,) and at Kyoto University (Researcher Hisashi Tadakuma), has developed a single-molecule imaging assay for observing target RNA cleavage by RISC in a test tube in real time for the first time, showing how RISC accurately cleaves and releases targets.

Specifically, their obsercations provide direct evidence for the model that the small RNA in the RISC consists of two parts, one of which quickly binds to the target RNA to be cleaved, while the other proofreads that the correct RNA has been found.

This groundbreaking result reveals RISC’s molecular mechanism of action and the illustration of this process was adopted as the cover design of this issue of the journal.

This achievement will also contribute to accelerating the research applications of RNAi such as to the development of RNA-based next-generation drugs, for example as gene therapy to suppress the production of a disease-causing protein.

Paper

Chunyan Yao, Hiroshi M Sasaki, Takuya Ueda, Yukihide Tomari and Hisashi Tadakuma, "Single-molecule analysis of the target cleavage reaction by Drosophila RNAi enzyme complex", Molecular Cell Online Edition: 2015/7/3 (Japan time), doi: 10.1016/j.molcel.2015.05.015.


Associated links
U Tokyo Research article

Euan McKay | ResearchSEA
Further information:
http://www.researchsea.com

More articles from Life Sciences:

nachricht Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory

nachricht How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>