Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

University of Iowa Researchers Identify Caffeine-Consuming Bacterium

09.06.2011
As it turns out, humans aren't the only organisms that turn to caffeine for a pick-me-up. University of Iowa scientists have identified four different bacteria that actually can live on caffeine.

One of them, known as Pseudomonas putida CBB5, was found in a flowerbed outside a UI research laboratory. The research team says the discovery -- and the new understanding of how the process works -- could in the future allow scientists to convert waste from leftover coffee, tea and even chocolate into useful substances, like pharmaceuticals, animal feed or biofuels.

Previous studies have also discovered caffeine-degrading bacteria, but the UI team took the research one step further. They identified the gene sequence that enables the bacterium to break down the caffeine compound in nature.

Caffeine is found naturally in more than 60 different plants and is composed of carbon, hydrogen, nitrogen and oxygen. Its molecular structure features three clusters of carbon and hydrogen atoms known as methyl groups, enabling caffeine to resist degradation by most bacteria.

Led by UI chemical and biochemical engineering doctoral student Ryan Summers, the study found that Pseudomonas putida CBB5 uses four newly discovered digestive proteins to break caffeine down into xanthine and then to carbon dioxide and ammonia. It removes the methyl groups from the molecule (a process called N-demethylation), allowing the bacteria to feed on the nitrogen atoms in the interior of the molecule (xanthine).

The caffeine digestive proteins from CBB5 can be used to convert caffeine into building blocks for drugs used to treat asthma, improve blood flow and stabilize heart arrhythmias.

"With one or two methyl groups removed, the remainder of the molecule can be used as the base for a number of pharmaceuticals," Summers said. "You basically use the new genes and enzymes that could take something we have a lot of -- like caffeine -- and make drugs that are typically very expensive. And that process could lower the costs for people who need them."

Summers said the bacterium's digestive proteins could also be used to remove caffeine and related compounds from large amounts of waste generated from coffee and tea processing, which pollute the environment. The decaffeinated waste from these industries could be used for animal feed, or for production of transportation fuel, especially in areas where corn (for ethanol) is scarce.

The team originally thought only one enzyme was responsible for extracting methyl groups. Ultimately, they identified four (NdmA, NdmB, NdmC, and reductase) involved in the N-demethylation process. This helped them to pinpoint the genes responsible for enzyme production in the bacterium.

Summers, with UI research scientists Michael Louie and Chi Li Yu, studied the bacterium in professor Mani Subramanian's lab in the Chemical and Biochemical Engineering Department, and the Center for Biocatalysis and Bioprocessing. They initially set out to craft a dipstick measurement for nursing mothers to test caffeine levels in breast milk, but the gene discovery took the research down a different path.

"These findings are a significant leap, as other researchers have shown bacteria can grow on caffeine, but, until now, the exact mechanism was a mystery," Subramanian said. "Now that we are starting to work on this, we are finding completely new genes, and reactions that we never expected."

Summers presented the findings at the American Society for Microbiology in New Orleans in late May. He anticipates seeking a scientific publication of the study this summer.

STORY SOURCE: University of Iowa Graduate College Office of External Relations, 205 Gilmore Hall, Iowa City, Iowa 52242-2500

Alison Sullivan | Newswise Science News
Further information:
http://www.uiowa.edu

More articles from Life Sciences:

nachricht Researchers develop eco-friendly, 4-in-1 catalyst
25.04.2017 | Brown University

nachricht Transfecting cells gently – the LZH presents a GNOME prototype at the Labvolution 2017
25.04.2017 | Laser Zentrum Hannover e.V.

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

7th International Conference on Crystalline Silicon Photovoltaics in Freiburg on April 3-5, 2017

03.04.2017 | Event News

 
Latest News

NASA's Fermi catches gamma-ray flashes from tropical storms

25.04.2017 | Physics and Astronomy

Researchers invent process to make sustainable rubber, plastics

25.04.2017 | Materials Sciences

Transfecting cells gently – the LZH presents a GNOME prototype at the Labvolution 2017

25.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>