Annia Rodriguez worked with John Perona, professor in UCSB's Department of Chemistry and Biochemistry, to decipher intramolecular communication within a large RNA-protein enzyme responsible for expressing the genetic code for the amino acid glutamine.
To their surprise, the experiments by Rodriguez captured a partial glimpse of how the genetic coding of life may have emerged. The results of the study are published in the journal Structure, published by CELL.
Life is based on the ability of all living cells to convert the genetic information in DNA, into the specific sequences of amino acids that make up the proteins that are the cell's workhorses. The key reaction in this decoding process is the attachment of a particular amino acid to one end of a small RNA molecule known as a transfer RNA. The enzyme that catalyzes this amino acid-RNA attachment is the aminoacyl-tRNA synthetase.
In all, Rodriguez found that separately removing seven different "gears" from a distant part of the molecule each caused the amino acid to bind more tightly to the aminoacyl-tRNA synthetase. Perona explained that this provides the first systematic analysis demonstrating long-range communication in an enzyme that depends on RNA for its function.
"So what we think is going on is that these enzyme-RNA interactions far from the amino acid binding site evolved together with the needs of the cell to respond to subtle cues from its environment – especially in terms of how much amino acid is available," said Perona. "It makes sense in terms of evolution."
Rodriguez is the first in her family to pursue a Ph.D., which she will complete this year. Now 28 years old, she began her career as a nurse in Cuernavaca, Mexico. Then she went on to obtain a B.S. in biochemical engineering at the Instituto Tecnológico de Zacatepec.
Graduation from her undergraduate program called for work at a research institution and she chose UCSB. Upon graduation, Rodriguez was offered a prestigious five-year scholarship with Mexico's Consejo Nacional de Ciencia y Technología (CONACYT) to continue her studies at UCSB.
Although her current research is not focused specifically on human health, Rodriguez said: "My interest in biochemistry started because I wanted to know the mechanisms by which drugs and medications worked inside the human body. I wanted to learn not just the signs and symptoms of disease, but how diseases are developed in a molecular level."
Gail Gallessich | EurekAlert!
New photocatalyst speeds up the conversion of carbon dioxide into chemical resources
29.05.2017 | DGIST (Daegu Gyeongbuk Institute of Science and Technology)
Copper hydroxide nanoparticles provide protection against toxic oxygen radicals in cigarette smoke
29.05.2017 | Johannes Gutenberg-Universität Mainz
The world's highest gain high power laser amplifier - by many orders of magnitude - has been developed in research led at the University of Strathclyde.
The researchers demonstrated the feasibility of using plasma to amplify short laser pulses of picojoule-level energy up to 100 millijoules, which is a 'gain'...
Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.
Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....
Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.
The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
24.05.2017 | Event News
23.05.2017 | Event News
22.05.2017 | Event News
29.05.2017 | Earth Sciences
29.05.2017 | Life Sciences
29.05.2017 | Physics and Astronomy