Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Trinity biochemists devise snappy new technique for blueprinting cell membrane proteins

08.06.2015

Biochemists from Trinity College Dublin have devised a new technique that will make the difficult but critical job of blueprinting certain proteins considerably faster, cheaper and easier.

The breakthrough will make a big splash in the field of drug discovery and development, where precise protein structure blueprints can help researchers understand how individual proteins work. Critically, these blueprints can show weaknesses that allow drug developers to draw up specific battle plans in the fight against diseases and infections.


The in meso in situ serial crystallography (IMISX) method allows researchers to quickly and accurately blueprint the 3-D structure of proteins.

Credit: Martin Caffrey (Trinity College Dublin).

Professor of Membrane Structural and Functional Biology at Trinity, Martin Caffrey, is the senior author of the research, which has just been published in the international peer-reviewed journal Acta Crystallographica D. He said:

"This is a truly exciting development. We have demonstrated the method on a variety of cell membrane proteins, some of which act as transporters. It will work with existing equipment at a host of facilities worldwide, and it is very simple to implement."

Over 50% of drugs on the market target cell membrane proteins, which are vital for the everyday functioning of complex cellular processes. They act as transporters to ensure that specific molecules enter and leave our cells, as signal interpreters important in decoding messages and initiating responses, and as agents that speed up appropriate responses.

The major challenge facing researchers is the production of large membrane protein crystals, which are used to determine the precise 3-D structural blueprints. That challenge has now been lessened thanks to the Trinity biochemists' advent - the in meso in situ serial crystallography (IMISX) method.

Beforehand, researchers needed to harvest protein crystals and cool them at inhospitable temperatures in a complex set of events that was damaging, inefficient and prone to error. The IMISX method allows researchers to determine structural blueprints as and where the crystals grow.

Professor Caffrey added: "The best part of this is that these proteins are as close to being 'live' and yet packaged in the crystals we need to determine their structure as they could ever be. As a result, this breakthrough is likely to supplant existing protocols and will make the early stages of drug development considerably more efficient."

The work was done in collaboration with scientists at the Swiss Light Source and the University of Konstanz and was supported by a grant from Science Foundation Ireland.

###

A pdf is available on request.

Media Contact

Thomas Deane
deaneth@tcd.ie
353-189-64685

 @tcddublin

http://www.tcd.ie/ 

Thomas Deane | EurekAlert!

More articles from Life Sciences:

nachricht 'Y' a protein unicorn might matter in glaucoma
23.10.2017 | Georgia Institute of Technology

nachricht Microfluidics probe 'cholesterol' of the oil industry
23.10.2017 | Rice University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Salmonella as a tumour medication

HZI researchers developed a bacterial strain that can be used in cancer therapy

Salmonellae are dangerous pathogens that enter the body via contaminated food and can cause severe infections. But these bacteria are also known to target...

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

3rd Symposium on Driving Simulation

23.10.2017 | Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

 
Latest News

Microfluidics probe 'cholesterol' of the oil industry

23.10.2017 | Life Sciences

Gamma rays will reach beyond the limits of light

23.10.2017 | Physics and Astronomy

The end of pneumonia? New vaccine offers hope

23.10.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>