BMBF funds joint project to develop new microscopy methods
Experts from science and industry are working on new methods of microscopy and advancing microscopy technology to improve our ability to study and understand the molecular processes of HIV infection.
Last year, the Federal Ministry of Education and Research (BMBF) approved approx. 4.6 million euros for this joint project called “Chemical Switches and Click Chemistry for High-resolution Microscopy”. Meanwhile preparations for the start of the project are complete.
The three-year “Switch Click Microscopy” project will involve scientists from Heidelberg University and Heidelberg University Hospital, the University of Würzburg, the European Molecular Biology Laboratory in Heidelberg as well as experts from four companies. The project coordinator is Prof. Dr. Dirk-Peter Herten, a member of the CellNetworks Cluster of Excellence who also works at the Institute for Physical Chemistry at Heidelberg University.
As Prof. Herten explains, there are more than 35 million people worldwide living with the human immunodeficiency virus HIV. “AIDS, the acquired immunodeficiency syndrome this virus causes, is now quite treatable in the industrialised world.
But the lifelong treatment with medication has to be continually adjusted. Because the virus is constantly changing, new treatment strategies are needed,” continues the Heidelberg scientist.
“By shedding light on how the virus functions and propagates, we can target our research to develop specific methods for diagnosis and treatment.” Researchers in the joint project are particularly focussed on changes in the T-cells of the immune system effected by the HIV Nef protein. In order to better investigate these processes, the team intends to greatly expand on previous methods of light microscopy.
“The key is the development of new fluorescent probes,” explains Prof. Herten. A new type of direct protein labelling should substantially improve the microscopic image and allow biological structures to be reconstructed in 3D super-resolution. To this end, the scientists intend to synthesise fluorescent probes whose properties can be controlled through chemical reactions.
“The goal is to selectively control the fluorescence by adding certain reagents,” stresses the project coordinator. “The new approach to super-resolution fluorescent microscopy can then function independently of light-driven processes.” Furthermore, the methods for protein labelling are to be improved to prevent artefacts and unspecific signals in the imaging.
The researchers are also targeting new developments in the field of microscopy technology, including methods to simplify adding reagents during microscopy as well as new light sources whose output and wavelengths are attuned to the newly developed fluorescent dyes.
Another field of endeavour are optical technologies that permit three-dimensional super-resolution imaging. According to Prof. Herten, these technical enhancements are to be based on standard microscopy systems to ensure wide-ranging and cost-efficient use. “Improving microscopic imaging makes understanding the biological processes surrounding the HIV immunodeficiency virus easier. This knowledge will allow us to focus the search for new AIDS drugs much more effectively.“
The industrial partners in the project include ATTO-TEC GmbH in Siegen, Sirius Fine Chemicals SiChem GmbH in Bremen als well as FEI Munich GmbH and TOPTICA Photonics AG in Munich. The research work at Heidelberg University will be supported through BMBF funds in the amount of approx. 670,000 euros.
Adjunct Professor Dr. Dirk-Peter Herten
CellNetworks Cluster of Excellence
Institute for Physical Chemistry
Phone: +49 6221 54-51220
Communications and Marketing
Press Office, phone: +49 6221 54-2311
Marietta Fuhrmann-Koch | idw - Informationsdienst Wissenschaft
Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...
UMD, NOAA collaboration demonstrates suitability of in-orbit datasets for weather satellite calibration
"Traffic and weather, together on the hour!" blasts your local radio station, while your smartphone knows the weather halfway across the world. A network of...
Fiber-reinforced plastics (FRP) are frequently used in the aeronautic and automobile industry. However, the repair of workpieces made of these composite materials is often less profitable than exchanging the part. In order to increase the lifetime of FRP parts and to make them more eco-efficient, the Laser Zentrum Hannover e.V. (LZH) and the Apodius GmbH want to combine a new measuring device for fiber layer orientation with an innovative laser-based repair process.
Defects in FRP pieces may be production or operation-related. Whether or not repair is cost-effective depends on the geometry of the defective area, the tools...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
16.01.2017 | Power and Electrical Engineering
16.01.2017 | Information Technology
16.01.2017 | Power and Electrical Engineering