Researchers identify a pheromone in the urine of male tilapia fish that stimulates spawning in females
The exchange of chemical signals between organisms is considered the oldest form of communication. Acting as messenger molecules, pheromones regulate social interactions between conspecifics, for example, the sexual attraction between males and females. Fish rely on pheromones to trigger social responses and to coordinate reproductive behavior in males and females.
Male Mozambique tilapia (Oreochromis mossambicus).
© Olinda G. Almeida / Peter C. Hubbard, Centre of Marine Sciences (CCMAR), Universität der Algarve, Faro, Portugal
Rival male Mozambique tilapias in an aquarium: The dominant male (right) defends the nest, an excavation in the sand he made with his mouth. Females take up the eggs in their mouths, once they have been externally fertilized in the nest by the males. Oral incubation is a strategy for protecting offspring.
© Peter C. Hubbard / Olinda G. Almeida, University of the Algarve, Faro, Portugal
Scientists at the Marine Science Center at the University of the Algarve in Faro, Portugal, and at the Max Planck Institute for Chemical Ecology in Jena, Germany, have now identified such a signal molecule in the urine of male Mozambique tilapia (Oreochromis mossambicus): this pheromone boosts hormone production and accelerates oocyte maturation in reproductive females. Hence, the Mozambique tilapia is one of the first fish species in which the chemical structure of a pheromone has been identified and the biological basis of its activity elucidated.
The social behavior of Mozambique tilapias (Oreochromis mossambicus) native to southern Africa is very complex. The strict hierarchic ranking among males is fought out in so-called courtship arenas. With their mouths, male tilapias make excavations in the sand in the middle of an arena with the aim of attracting females to spawn in these nests.
At the same time, they act aggressively to keep other males away. Dominant males have been observed to urinate more often and squirt larger quantities of urine in the water during fights compared to their subordinate rivals. The urine contains pheromones that reduce aggressive behavior in other males. The compounds also lure females to the nest and modify their hormonal status by accelerating oocyte maturation. Thus the pheromones help to synchronize female spawning and external fertilization by the males and so to increase the odds of reproductive success.
Tilapias also show this behavior in captivity, which makes them an ideal model system for reproducible biological assays. Tina Keller-Costa and her colleagues at the Marine Science Center at the University of the Algarve in Faro, Portugal, as well as the Biosynthesis/NMR Research Group at the Max Planck Institute for Chemical Ecology in Jena, Germany, have now identified the chemical structure of the signaling molecules in tilapia males and studied their function. She collected urine samples from dominant males and purified the samples in several steps, testing them for biological activity as pheromones after each step.
This procedure resulted in two pure substances whose chemical structures were elucidated using nuclear magnetic resonance spectroscopy (NMR). Their identity was confirmed by chemical synthesis: “The two structures are stereoisomers or mirror images of a pregnane-type steroid linked to glucuronic acid,” Bernd Schneider, the head of the NMR lab in Jena, said, summarizing the results of the analysis.
Both males and females showed highly sensitive responses to the odor of these two steroids. Although the two pheromone components stimulate the hormonal system of the females and trigger spawning, they are not by themselves capable of reducing aggressive behavior in rival males. The researchers thus assume that the urine of dominant males must contain additional yet-to-be identified substances that contribute to this effect in a complex mixture.
Until now only very few fish pheromones have been chemically identified. “Our discovery will allow for further investigations, for example, of the mechanisms of perception and processing of these chemical signals by the brain in order to originate a response, in this case oocyte maturation and behavioral changes,” says Tina Keller-Costa, who conducted the experiments for her PhD thesis.
Apart from the carp family, tilapias belong to the most important edible fish raised commercially. However, their aquaculture in many tropical and subtropical waters has led to an uncontrolled population growth and spread of the species. The use of pheromones could help optimize the aquaculture of tilapia species by increasing female fertility and reducing aggression between competing males. Pheromones could also help to control the invasive behavior of these fish, which threatens the ecological balance of many ecosystems.
Dr. Bernd Schneider | Max-Planck-Institut
MACC1 Gene Is an Independent Prognostic Biomarker for Survival in Klatskin Tumor Patients
31.08.2015 | Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft
Fish Oil-Diet Benefits May be Mediated by Gut Microbes
28.08.2015 | University of Gothenburg
The leaves of the lotus flower, and other natural surfaces that repel water and dirt, have been the model for many types of engineered liquid-repelling surfaces. As slippery as these surfaces are, however, tiny water droplets still stick to them. Now, Penn State researchers have developed nano/micro-textured, highly slippery surfaces able to outperform these naturally inspired coatings, particularly when the water is a vapor or tiny droplets.
Enhancing the mobility of liquid droplets on rough surfaces could improve condensation heat transfer for power-plant heat exchangers, create more efficient...
Longer, more severe, and hotter droughts and a myriad of other threats, including diseases and more extensive and severe wildfires, are threatening to transform some of the world's temperate forests, a new study published in Science has found. Without informed management, some forests could convert to shrublands or grasslands within the coming decades.
"While we have been trying to manage for resilience of 20th century conditions, we realize now that we must prepare for transformations and attempt to ease...
A University of Oklahoma astrophysicist and his Chinese collaborator have found two supermassive black holes in Markarian 231, the nearest quasar to Earth, using observations from NASA's Hubble Space Telescope.
The discovery of two supermassive black holes--one larger one and a second, smaller one--are evidence of a binary black hole and suggests that supermassive...
A team of European researchers have developed a model to simulate the impact of tsunamis generated by earthquakes and applied it to the Eastern Mediterranean. The results show how tsunami waves could hit and inundate coastal areas in southern Italy and Greece. The study is published today (27 August) in Ocean Science, an open access journal of the European Geosciences Union (EGU).
Though not as frequent as in the Pacific and Indian oceans, tsunamis also occur in the Mediterranean, mainly due to earthquakes generated when the African...
In mountainous regions earthquakes often cause strong landslides, which can be exacerbated by heavy rain. However, after an initial increase, the frequency of these mass wasting events, often enormous and dangerous, declines, in fact independently of meteorological events and aftershocks.
These new findings are presented by a German-Franco-Japanese team of geoscientists in the current issue of the journal Geology, under the lead of the GFZ...
20.08.2015 | Event News
20.08.2015 | Event News
19.08.2015 | Event News
01.09.2015 | Press release
01.09.2015 | Materials Sciences
01.09.2015 | Materials Sciences