Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The Combined Nanoscopy Technique

18.08.2014

Göttingen Scientists develop a combined technique for studying cellular structures via high-resolution imaging. Published in Nature Communications.

Original publication:
Saka SK, Vogts A, Kröhnert K, Hillion F, Rizzoli SO*, Wessels J* (2014). Correlated optical and isotopic nanoscopy. NAT COMMUN, 5: 3664.

Molecular processes in living cells can best be monitored by high-resolution microscopy techniques. Although groundbreaking technical innovations in the field of microscopy have been made in the past, frontiers still exist.


From left to right: Dr. Johannes Wessels, Dr. Sinem K. Saka, Katharina Kröhnert, Prof. Dr. Silvio O. Rizzoli. Prof. Rizzoli / CNMPB

Prof. Dr. Silvio O. Rizzoli and his team of the Göttingen DFG Research Center and Cluster of Excellence for Nanoscale Microscopy and Molecular Physiology of the Brains (CNMPB) have now developed a new application by combining two imaging techniques to expand the benefits of high-resolution to study biological questions.

The new imaging technique COIN enables to study the turnover and metabolism of subcellular structures, such as organelles, in detail. The new method has been described in Nature Communications.

The turnover of subcellular organelles is one of the least understood aspects of modern cell biology, despite its widely recognized importance. In biology, these processes are studied by “feeding” cells with marker molecules such as amino acids labeled with stable isotopes.

Over time these amino acids are metabolically incorporated into cellular proteins and the isotopic composition can then be imaged by secondary ion mass spectrometry (SIMS). This technique enables visualization of different organelles in cells and tissues. However, SIMS by itself cannot identify specific subcellular structures.

Therefore, the team of Prof. Rizzoli in collaboration with scientists of the Leibniz Institute for Baltic Sea Research in Warnemünde and the French company Cameca successfully correlated SIMS with a second technique.

The combined method termed “correlated optical and isotopic nanoscopy (COIN)” is based on super-resolution stimulated emission depletion (STED) microscopy. COIN allows precise studies of the protein turnover in different single organelles from cultured hippocampal neurons. The new approach has been recently published in Nature Communications.

Each of the combined techniques alone provides a piece of information that is unavailable for the other: “SIMS yields the isotopic composition of the material investigated and even its turnover, while STED microscopy reveals the identities and the spatial distribution of organelles or organelle components.”, Prof. Rizzoli explains. The combination (COIN) for the first time allows precisely determining the turnover of proteins in various single organelles in cells.

A special feature of the technique is the wide-range application to a variety of biological samples, which should therefore enable the investigation of the composition of many organelles and sub-cellular structures. Using COIN the scientists successfully yielded information about the protein turnover in different organelles of cultured hippocampal neurons. COIN can be applied to a variety of biological samples, and should therefore enable the investigation of the isotopic composition of many organelles and subcellular structures.

Prof. Dr. Silvio O. Rizzoli is head of the Department of Neuro- and Sensory Physiology at the University Medical Center Göttingen and member of the Göttingen Cluster of Excellence and DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB).

His research focus includes the identification of molecular signal transduction processes between nerve cells. Prof. Rizzoli applies super-resolution microscopy techniques to study the transport and function of intracellular vesicles in the synapses of nerve cells. Very recently, Prof. Rizzoli received for the second time a prestigious funding award of the European Union for his excellent research proposal.

INFORMATION
CNMPB: http://www.cnmpb.de
Prof. Dr. Silvio O. Rizzoli
University Medical Center Göttingen Department Neuro- & Sensory Physiology c/o European Neuroscience Institute (ENI) Grisebachstraße 5, 37077 Göttingen Telefon 0551 / 39-33630, srizzol@gwdg.de

CNMPB – Center for Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 – DFG Research Center 103
Dr. Heike Conrad
Scientific Coordination, Press & Public Relations
Humboldtallee 23, 37073 Göttingen
Telefon 0551 / 39-7065, heike.conrad@med.uni-goettingen.de

Leibniz-Institut für Ostseeforschung Warnemünde
Sektion Biologische Meereskunde
Dr. Angela Vogts, Telefon 0381 / 5197 353, angela.vogts@io-warnemuende.de

Weitere Informationen:

http://rizzoli-lab.de - Department Prof. S. O. Rizzoli
http://www.cnmpb.de - Cluster of Excellence and DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
http://www.io-warnemuende.de/en_index.html - Leibniz Institute for Baltic Sea Research Warnemünde

Dr. Heike Conrad | idw - Informationsdienst Wissenschaft

Further reports about: Brain CNMPB COIN DFG Microscopy Molecular SIMS composition organelles processes structures

More articles from Life Sciences:

nachricht Newly designed molecule binds nitrogen
23.02.2018 | Julius-Maximilians-Universität Würzburg

nachricht Atomic Design by Water
23.02.2018 | Max-Planck-Institut für Eisenforschung GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Attoseconds break into atomic interior

A newly developed laser technology has enabled physicists in the Laboratory for Attosecond Physics (jointly run by LMU Munich and the Max Planck Institute of Quantum Optics) to generate attosecond bursts of high-energy photons of unprecedented intensity. This has made it possible to observe the interaction of multiple photons in a single such pulse with electrons in the inner orbital shell of an atom.

In order to observe the ultrafast electron motion in the inner shells of atoms with short light pulses, the pulses must not only be ultrashort, but very...

Im Focus: Good vibrations feel the force

A group of researchers led by Andrea Cavalleri at the Max Planck Institute for Structure and Dynamics of Matter (MPSD) in Hamburg has demonstrated a new method enabling precise measurements of the interatomic forces that hold crystalline solids together. The paper Probing the Interatomic Potential of Solids by Strong-Field Nonlinear Phononics, published online in Nature, explains how a terahertz-frequency laser pulse can drive very large deformations of the crystal.

By measuring the highly unusual atomic trajectories under extreme electromagnetic transients, the MPSD group could reconstruct how rigid the atomic bonds are...

Im Focus: Developing reliable quantum computers

International research team makes important step on the path to solving certification problems

Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...

Im Focus: In best circles: First integrated circuit from self-assembled polymer

For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.

In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Basque researchers turn light upside down

23.02.2018 | Physics and Astronomy

Finnish research group discovers a new immune system regulator

23.02.2018 | Health and Medicine

Attoseconds break into atomic interior

23.02.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>