Göttingen Scientists develop a combined technique for studying cellular structures via high-resolution imaging. Published in Nature Communications.
Saka SK, Vogts A, Kröhnert K, Hillion F, Rizzoli SO*, Wessels J* (2014). Correlated optical and isotopic nanoscopy. NAT COMMUN, 5: 3664.
Molecular processes in living cells can best be monitored by high-resolution microscopy techniques. Although groundbreaking technical innovations in the field of microscopy have been made in the past, frontiers still exist.
Prof. Dr. Silvio O. Rizzoli and his team of the Göttingen DFG Research Center and Cluster of Excellence for Nanoscale Microscopy and Molecular Physiology of the Brains (CNMPB) have now developed a new application by combining two imaging techniques to expand the benefits of high-resolution to study biological questions.
The new imaging technique COIN enables to study the turnover and metabolism of subcellular structures, such as organelles, in detail. The new method has been described in Nature Communications.
The turnover of subcellular organelles is one of the least understood aspects of modern cell biology, despite its widely recognized importance. In biology, these processes are studied by “feeding” cells with marker molecules such as amino acids labeled with stable isotopes.
Over time these amino acids are metabolically incorporated into cellular proteins and the isotopic composition can then be imaged by secondary ion mass spectrometry (SIMS). This technique enables visualization of different organelles in cells and tissues. However, SIMS by itself cannot identify specific subcellular structures.
Therefore, the team of Prof. Rizzoli in collaboration with scientists of the Leibniz Institute for Baltic Sea Research in Warnemünde and the French company Cameca successfully correlated SIMS with a second technique.
The combined method termed “correlated optical and isotopic nanoscopy (COIN)” is based on super-resolution stimulated emission depletion (STED) microscopy. COIN allows precise studies of the protein turnover in different single organelles from cultured hippocampal neurons. The new approach has been recently published in Nature Communications.
Each of the combined techniques alone provides a piece of information that is unavailable for the other: “SIMS yields the isotopic composition of the material investigated and even its turnover, while STED microscopy reveals the identities and the spatial distribution of organelles or organelle components.”, Prof. Rizzoli explains. The combination (COIN) for the first time allows precisely determining the turnover of proteins in various single organelles in cells.
A special feature of the technique is the wide-range application to a variety of biological samples, which should therefore enable the investigation of the composition of many organelles and sub-cellular structures. Using COIN the scientists successfully yielded information about the protein turnover in different organelles of cultured hippocampal neurons. COIN can be applied to a variety of biological samples, and should therefore enable the investigation of the isotopic composition of many organelles and subcellular structures.
Prof. Dr. Silvio O. Rizzoli is head of the Department of Neuro- and Sensory Physiology at the University Medical Center Göttingen and member of the Göttingen Cluster of Excellence and DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB).
His research focus includes the identification of molecular signal transduction processes between nerve cells. Prof. Rizzoli applies super-resolution microscopy techniques to study the transport and function of intracellular vesicles in the synapses of nerve cells. Very recently, Prof. Rizzoli received for the second time a prestigious funding award of the European Union for his excellent research proposal.
Prof. Dr. Silvio O. Rizzoli
University Medical Center Göttingen Department Neuro- & Sensory Physiology c/o European Neuroscience Institute (ENI) Grisebachstraße 5, 37077 Göttingen Telefon 0551 / 39-33630, email@example.com
CNMPB – Center for Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 – DFG Research Center 103
Dr. Heike Conrad
Scientific Coordination, Press & Public Relations
Humboldtallee 23, 37073 Göttingen
Telefon 0551 / 39-7065, firstname.lastname@example.org
Leibniz-Institut für Ostseeforschung Warnemünde
Sektion Biologische Meereskunde
Dr. Angela Vogts, Telefon 0381 / 5197 353, email@example.com
http://rizzoli-lab.de - Department Prof. S. O. Rizzoli
http://www.cnmpb.de - Cluster of Excellence and DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
http://www.io-warnemuende.de/en_index.html - Leibniz Institute for Baltic Sea Research Warnemünde
Dr. Heike Conrad | idw - Informationsdienst Wissenschaft
Billions of juvenile fish under the Arctic sea ice
12.10.2015 | Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung
Indications of spontaneous formation of precursor proteins
12.10.2015 | Universität Stuttgart
Having a light touch can make a hefty difference in how well animals and robots move across challenging granular surfaces such as snow, sand and leaf litter. Research reported October 9 in the journal Bioinspiration & Biomimetics shows how the design of appendages – whether legs or wheels – affects the ability of both robots and animals to cross weak and flowing surfaces.
Using an air fluidized bed trackway filled with poppy seeds or glass spheres, researchers at the Georgia Institute of Technology systematically varied the...
Nondestructive material testing (NDT) is a fast and effective way to analyze the quality of a product during the manufacturing process. Because defective materials can lead to malfunctioning finished products, NDT is an essential quality assurance measure, especially in the manufacture of safety-critical components such as automotive B-pillars. NDT examines the quality without damaging the component or modifying the surface of the material. At this year's Blechexpo trade fair in Stuttgart, Fraunhofer IZFP will have an exhibit that demonstrates the nondestructive testing of high-strength automotive body parts using 3MA. The measurement results are available in a matter of seconds.
To minimize vehicle weight and fuel consumption while providing the highest level of crash safety, automotive bodies are reinforced with elements made from...
The MICADO camera, a first light instrument for the European Extremely Large Telescope (E-ELT), has entered a new phase in the project: by agreeing to a Memorandum of Understanding, the partners in Germany, France, the Netherlands, Austria, and Italy, have all confirmed their participation. Following this milestone, the project's transition into its preliminary design phase was approved at a kick-off meeting held in Vienna. Two weeks earlier, on September 18, the consortium and the European Southern Observatory (ESO), which is building the telescope, have signed the corresponding collaboration agreement.
As the first dedicated camera for the E-ELT, MICADO will equip the giant telescope with a capability for diffraction-limited imaging at near-infrared...
Self-driving cars will be on our streets in the foreseeable future. In Graz, research is currently dedicated to an innovative driver assistance system that takes over control if there is a danger of collision. It was nature that inspired Dr Manfred Hartbauer from the Institute of Zoology at the University of Graz: in dangerous traffic situations, migratory locusts react around ten times faster than humans. Working together with an interdisciplinary team, Hartbauer is investigating an affordable collision detector that is equipped with artificial locust eyes and can recognise potential crashes in time, during both day and night.
Inspired by insects
An interdisciplinary team of researchers has built the first prototype of a miniature particle accelerator that uses terahertz radiation instead of radio...
01.10.2015 | Event News
30.09.2015 | Event News
17.09.2015 | Event News
12.10.2015 | Press release
12.10.2015 | Press release
12.10.2015 | Physics and Astronomy