Nerve cells containing the light-activated 5-HT1A receptor (green) and the naturally occuring 5-HT1A receptor (red)
© RUB, Bild: Masseck
One receptor, which is important for the regulation of serotonin levels in the brain, is the 5-HT1A receptor. It belongs to a protein family called G protein coupled receptors (GPCRs). These receptors can activate different signalling pathways in cells to support or suppress various signalling events.
“About 30 per cent of the current drugs target specifically GPCRs”, says Prof Dr Stefan Herlitze from the Department of General Zoology and Neurobiology at the RUB. Due to the lack of tools to control intracellular signalling pathways with high temporal and spatial accuracy, it was so far difficult to analyse these pathways precisely.
Coupling of visual pigments to serotonin receptors
Applying optogenetic methods the scientists in Bochum used cone opsins from the mouse and human eye to control specifically serotonin signalling pathways either with blue or red light. Prof Dr Stefan Herlitze has been working with optogenetic techniques since 2005 and is one of the pioneers in the field. The light-activated serotonin receptors can be switched on within milliseconds, are extremely light sensitive in comparison to other optogenetic tools and can be repetitively activated. “We hope that with the help of these optogenetic tools, we will be able to gain a better understanding about how anxiety and depression originate”, states RUB neuroscientist Dr Olivia Masseck.
Successful behavioural tests
The scientists also demonstrated that they were able to modulate mouse emotional behaviour using the light-activated receptors. When they switched on the serotonergic signals by light in a certain brain area, the mice became less anxious.
O.A. Masseck, K. Spoida, D. Dalkara, T. Maejima, J.M. Rubelwoski, L. Wallhorn, E.S. Deneris, S. Herlitze (2014): Vertebrate cone opsins enable sustained and highly sensitive rapid control of Gi/o signaling in anxiety circuitry. Neuron, DOI: 10.1016/j.neuron.2014.01.041
Dr. Olivia Masseck | EurekAlert!
Rapid adaptation to a changing environment
28.04.2016 | Christian-Albrechts-Universität zu Kiel
Tiny microscopes reveal hidden role of nervous system cells
28.04.2016 | Salk Institute
Neutron scattering and computational modeling have revealed unique and unexpected behavior of water molecules under extreme confinement that is unmatched by any known gas, liquid or solid states.
In a paper published in Physical Review Letters, researchers at the Department of Energy's Oak Ridge National Laboratory describe a new tunneling state of...
Honeycomb structures as the basic building block for industrial applications presented using holo pyramid
Researchers of the Alfred Wegener Institute (AWI) will introduce their latest developments in the field of bionic lightweight design at Hannover Messe from 25...
Polymer solar cells can be even cheaper and more reliable thanks to a breakthrough by scientists at Linköping University and the Chinese Academy of Sciences (CAS). This work is about avoiding costly and unstable fullerenes.
Polymer solar cells can be even cheaper and more reliable thanks to a breakthrough by scientists at Linköping University and the Chinese Academy of Sciences...
As one of the leading R&D partners in the development of surface technologies and organic electronics, the Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP will be exhibiting its recent achievements in vacuum coating of ultra-thin glass at SVC TechCon 2016 (Booth 846), taking place in Indianapolis / USA from May 9 – 13.
Fraunhofer FEP is an experienced partner for technological developments, known for testing the limits of new materials and for optimization of those materials...
Liquid water is a very good heat storage medium – anyone with a Thermos bottle knows that. However, as soon as water boils or freezes, its storage capacity drops precipitously. Physicists at the University of Bonn have now observed very similar behavior in a gas of light particles. Their findings can be used, for example, to produce ultra-precise thermometers. The work appears in the prestigious technical journal "Nature Communications".
Water vapor becomes liquid under 100 degrees Celsius – it condenses. Physicists speak of a phase transition. In this process, certain thermodynamic...
27.04.2016 | Event News
15.04.2016 | Event News
12.04.2016 | Event News
28.04.2016 | Physics and Astronomy
28.04.2016 | Materials Sciences
28.04.2016 | Life Sciences