Spectrin, F-actin, and associated proteins form a meshwork that supports and shapes the plasma membrane of red blood cells. A similar network underlies the membranes of other cell types, including lens fiber cells: elongated epithelial cells that encircle vertebrate lenses in concentric layers, appearing in cross section as tightly packed hexagons. Actin filaments within this membrane skeleton are stabilized by their association with members of the tropomyosin and tropomodulin families of actin-binding proteins.
In mice lacking tropomodulin1, gamma-tropomyosin was also lost from the membrane skeleton of lens fiber cells. F-actin and spectrin remained associated with the cell membrane, but gaps appeared in the usually continuous protein network, suggesting that the two actin-binding proteins stabilize a subset of actin filaments required to link the network together. Scanning electron microscopy revealed that fiber cell membrane protrusions, which interlock with neighboring cells, were distorted and irregularly arranged in the absence of tropomodulin1. And although the fiber cells appeared hexagonal when first forming at the lens' equator, they often became misshapen and disorganized as they matured and moved toward the lens' center.
Senior author Velia Fowler thinks that disruption of the spectrin–actin network alters the adhesive interactions between neighboring cells, causing their shapes and packing to become disordered in response to the mechanical stresses associated with lens growth and eye movements.
About the Journal of Cell Biology
Founded in 1955, the Journal of Cell Biology (JCB) is published by the Rockefeller University Press. All editorial decisions on manuscripts submitted are made by active scientists in conjunction with our in-house scientific editors. JCB content is posted to PubMed Central, where it is available to the public for free six months after publication. Authors retain copyright of their published works and third parties may reuse the content for non-commercial purposes under a creative commons license. For more information, please visit www.jcb.org.
Nowak, R.B., et al. 2009. J. Cell Biol. doi:10.1083/jcb.200905065.
Rita Sullivan | EurekAlert!
Complementing conventional antibiotics
24.05.2018 | Goethe-Universität Frankfurt am Main
Building a brain, cell by cell: Researchers make a mini neuron network (of two)
23.05.2018 | Institute of Industrial Science, The University of Tokyo
A research team led by physicists at the Technical University of Munich (TUM) has developed molecular nanoswitches that can be toggled between two structurally different states using an applied voltage. They can serve as the basis for a pioneering class of devices that could replace silicon-based components with organic molecules.
The development of new electronic technologies drives the incessant reduction of functional component sizes. In the context of an international collaborative...
At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.
At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...
There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?
At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
24.05.2018 | Ecology, The Environment and Conservation
24.05.2018 | Medical Engineering
24.05.2018 | Physics and Astronomy