The study was conducted by a group of Korean scientists and will be awarded 3rd prize for best abstract in non-oncology research on the opening day of the congress.
During their investigation, the group aimed to examine the differentiation of human mesenchymal stem cells cultivated on the surface of nanofibrous meshes (nano-hMSCs) into neuron-like cells and repair of erectile dysfunction using their transplantation around the injured cavernous nerve (CN) of rats.
"The objectives of the study reflect a very pertinent need in today's urology practice," said the lead author of the investigation Prof. Y.S. Song of Soonchunhyang University School of Medicine in South Korea. "Post-prostatectomy erectile dysfunction results from injury to the cavernous nerve that provides the autonomic input to erectile tissue. It is a common complication after radical prostatectomy which decreases the patient's quality of life".
"Although advances in equipment and surgical techniques reduce this complication, patients still experience erectile dysfunction after radical prostatectomy," he explained.
Treatment of phosphodiesterase 5 inhibitors shows insufficient effectiveness in the treatment of post-prostatectomy ED and it is believed that the transplantation of stem cells cultivated on the surface of nanofibrous meshes can promote cavernous neuronal regeneration and repair erectile dysfunction.
In the course of the study, the synthesised polymer was electrospun in a rotating drum to prepare nanofibrous meshes and hMSCs were prepared and confirmed. Eight week old male Sprague-Dawley rats were divided into 4 groups of 10 each, including sham operation (group 1), CN injury (group 2), hMSCs treatment after CN injury (group 3) and nano-hMSCs treatment after CN injury (group 4). Immediately after the CN injury in group 4, nano-hMSCs encircled the injured CN. Erectile response was assessed by CN stimulation at 2, 4 weeks. Thereafter, penile tissue samples were harvested and examined using morphological analysis and immuno-histochemical stain against nerves (nestin, tubulin âIII and map2), endothelium (CD31,vWF) and smooth muscle (smooth muscle actin).
The results of the study revealed that at 2, 4 weeks, transplantation of nano-hMSCs increased the expression levels of cavernous neuronal, endothelial and smooth muscle makers more than hMSCs alone.
Additionally, nano-hMSCs increased the neuronal differentiation of mesenchymal stem cells more than hMSCs alone. At 2, 4 weeks, the mean percent collagen area of caversnosum increased following CN injury and recovered after transplantation of nano-hMSCs more than hMSCs alone.At 2, 4 weeks, the group with CN injury had significantly lower erectile function than the group without CN injury (p
The authors of the study concluded that nano-hMSCs differentiated into neuron-like cells and their transplantation repair erectile dysfunction in the rats with CN injury. These findings have high potential for the development of follow-up research projects.
"The outcomes of the current study could be a starting point for investigating clinical application of autologous adipocyte derived mesenchymal stem cells cultivated on the nonofiber to the injured caverneous nerve after radical prostatectomy," said Prof. Song.
"This is necessary to evaluate the effectiveness and safety of transplantated human mesenchymal stem cells cultivated on the surface of nanofibrous meshes against post-prostatectomy erectile dysfunction in patients with cavernous nerve injury."
Ivanka Moerkerken | EurekAlert!
Unique genome architectures after fertilisation in single-cell embryos
30.03.2017 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH
Transport of molecular motors into cilia
28.03.2017 | Aarhus University
The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
30.03.2017 | Health and Medicine
30.03.2017 | Health and Medicine
30.03.2017 | Medical Engineering