Researchers aimed to investigate whether the mononuclear cell fraction of human cord blood (HUCBM cells), which contains stem cells, might be useful in hepatic regenerative medicine. Both histological and biochemical findings obtained in this research suggest that cell transplantation did not improve the health of sick animals but it induced a hepatorenal syndrome instead.
The authors of this work are Ana I Álvarez-Mercado, María V García-Mediavilla, Sonia Sánchez-Campos, Francisco Abadía, María J Sáez-Lara, María Cabello-Donayre, Ángel Gil, Javier González-Gallego and Luis Fontana, researchers from the University of Granada and University of León.
Research in rats
In order to evaluate the regenerative potential of HUCBM cells, researchers carried out a human-to-rat xenograft. First, liver cirrhosis was induced to rats by administration of 0.3 g/L thioacetamide (TAA) in drinking water throughout 4 months. Later on, ten million HUCBM cells were injected through the portal vein. A similar transplantation experiment was done in control rats, i. e., rats that drank water, not TAA.
TAA induced nodular cirrhosis to animals. Cell therapy did not have any effect on hepatic histology, but analysis of biochemical parameters revealed that cirrhotic rats subjected to transplantation exhibited alterations in liver fuction (lower albumin concentration and higher bilirubin concentration in plasma compared to cirrhotic rats that did not receive HUCBM cells). Also, the group with cirrhosis that received HUCBM cells showed renal damage.
Nowadays, approximately 17% of the world population is affected by liver diseases. There is to date no specific treatment for the liver fibrosis that develops in chronic hepatic diseases, and patients receive treatment for its associated complications. In addition, the current therapy for end-stage hepatic disease, whole liver transplantation, is limited by the shortage of organ donors. Accordingly, novel therapies, such as the use of cord blood stem cells, are required to alleviate the suffering of many patients. This work, however, highlights the need of further research in the area of hepatic regenerative medicine.
The work has been funded by Fondo de Investigación Sanitaria (Instituto de Salud Carlos III), FEDER, Consejería de Innovación, Ciencia y Empresa (Junta de Andalucía), Consejería de Sanidad (Junta de Castilla y León), and Federación de Cajas de Ahorro de Castilla y León. It will appear in the November issue of the journal Cell Transplantation.
Contact: Dr. Luis Fontana. Dept. Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada. Phone: +34-958-242335. E-mail: email@example.com.
A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
18.08.2017 | Life Sciences
18.08.2017 | Physics and Astronomy
18.08.2017 | Materials Sciences