A technique developed by University of Central Florida scientists would allow relief workers to test water sources that could be contaminated with the cholera toxin. In the test, the sugar dextran is coated onto iron oxide nanoparticles and then added to a sample of the water. If the cholera toxin is present, the toxin will bind to the nanoparticles' dextran. This is because dextran looks similar to the cholera toxin receptor (GM1) found on cells' surface in the victim's gut.
The technique likely would be less expensive than those currently available, and it would provide results more quickly, enabling workers to restrict access to contaminated sources and limit the spread of the disease.
"It's really quite amazing," said UCF assistant professor J. Manuel Perez, the lead researcher on the project. "It means we have a quicker diagnostic tool using a simple and relatively cheap sugar-nanoparticle combination."
Early studies also show that the technique could someday be used to treat someone infected with cholera, which is caused by poor sanitation and dirty water, and potentially other diseases, Perez said.
More studies are needed to prove the adaptability of the technique, but its impact could be huge. In countries with poor sanitation, outbreaks caused by drinking contaminated water often prove fatal. Deadly toxins also can result from bioterrorism or food contamination.
There are an estimated 3 million to 5 million cholera cases, and 100,000 to 120,000 deaths, worldwide each year, according to the World Health Organization. A cholera outbreak has killed more than 3,000 people in Haiti since the earthquake, and WHO warned earlier in January that the outbreak has not yet reached its peak.
Research findings appear online today in the journal Bioconjugate Chemistry. (http://pubs.acs.org/doi/abs/10.1021/bc100442q). The National Institute of General Medical Sciences at the National Institutes of Health funded the research.
The findings may give the Federal Drug Administration, Centers for Disease Control and Prevention and several other agencies additional screening tools to combat toxins. The UCF-developed technique is faster than current detection methods, and it would likely be less expensive because these nanoparticles are cheap to make in large quantities. The detection instruments are compact in some cases the size of a desktop computer and a handheld calculator, and they could be turned into mobile devices that relief workers or food screeners could use in the field.
"As we have seen in the 2010 outbreak in Haiti, cholera remains a serious threat," said Janna Wehrle, Ph.D., of the National Institutes of Health, who oversees Dr. Perez's and other grants that focus on protein structures and interactions. "By developing a fast and sensitive test for cholera toxin that does not require sophisticated equipment or refrigeration, Drs. Perez and Teter have provided health care workers with a potentially valuable tool for use in areas struck by natural disasters or with inadequate infrastructure. The possibility that the novel chemistry discovered by these investigators might also be useful for treating cholera is especially exciting."
Studies that are under way may confirm early indications that dextran can be an effective drug for patients infected with cholera, added UCF Associate Professor Kenneth Teter, a co-author on the study. This could be especially beneficial in developing countries such as Haiti, as dextran is a relatively inexpensive compound to produce.
Additionally, both dextran and iron oxide are commonly used in other medical applications. Dextran is often used to prevent blood clots anticoagulant and in emergency treatments of hemorrhagic and traumatic shock. Iron oxide nanoparticles are used to treat anemia and as MRI contrast agents to achieve improved anatomical imaging.
Other contributors on the research team include postdoctoral fellows Charalambos Kaittanis and Santimukul Santra of UCF's NanoScience Technology Center, graduate student Oscar Santiesteban of the Chemistry Department and postdoctoral fellow Tuhina Banerjee from the Burnett School of Biomedical Sciences.
Perez, a Puerto Rico native, has broad experience in the academic, research and corporate worlds. A UCF professor since 2005, he works at the NanoScience Technology Center and in the Chemistry Department and Burnett School of Biomedical Sciences in the College of Medicine. He has a Ph.D. from Boston University in Biochemistry, and he completed postdoctoral training at Massachusetts General Hospital, Harvard Medical School's teaching and research hospital. He also has worked for the Millipore Corporation in Bedford, Mass.
At UCF, Perez has written numerous articles in prestigious journals such as Nature Materials, Nanoletters, Small, PLoS One, ACS Nano and Angewandte Chemie Int. Ed and JACS.
UCF Stands For Opportunity --The University of Central Florida is a metropolitan research university that ranks as the second largest in the nation with more than 56,000 students. UCF's first classes were offered in 1968. The university offers impressive academic and research environments that power the region's economic development. UCF's culture of opportunity is driven by our diversity, Orlando environment, history of entrepreneurship and our youth, relevance and energy. For more information visit http://news.ucf.edu
NIGMS is a part of NIH that supports basic research to increase our understanding of life processes and lay the foundation for advances in disease diagnosis, treatment and prevention. For more information on the Institute's research and training programs, see http://www.nigms.nih.gov.
The National Institutes of Health (NIH)-The Nation's Medical Research Agency-includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. It is the primary Federal agency for conducting and supporting basic, clinical, and translational medical research, and it investigates the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit www.nih.gov.
'Lipid asymmetry' plays key role in activating immune cells
20.02.2018 | Biophysical Society
New printing technique uses cells and molecules to recreate biological structures
20.02.2018 | Queen Mary University of London
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.
But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...
Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.
The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...
Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters
Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
20.02.2018 | Life Sciences
20.02.2018 | Medical Engineering
20.02.2018 | Physics and Astronomy