A characteristic feature of L. monocytogenes is its ability to grow at refrigeration temperatures and in the presence of high concentrations of salt—traditional food preservation techniques, which arrest the growth of most other pathogens.
Work in the Sleator lab has shown that the bacterium protects itself from such stresses by twisting into a protective corkscrew type shape in an effort to reduce its exposure to the stress—in the same way a human might wrap up tight—hugging the core to reduce the effects of the cold.
Furthermore, Sleator and colleagues have identified a single point mutation (out of a total of 3 million or so nucleotides that constitute the entire listerial genome), which dramatically improves the growth of the pathogen in the refrigerator.
The research paper, "A single point mutation in the listerial betL óA-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity," will be published in the November/December 2013 issue of Bioengineered. It is available open access ahead of press: http://www.landesbioscience.com/journals/bioe/article/24094/
Sleator claims that this mutation represents "a double edged sword;" "from a food safety perspective, a single point mutation with the potential to induce such dramatic shifts in cell growth and survival at low temperatures—making an already dangerous pathogen even more formidable—raises significant food-safety concerns which need to be addressed." However, from a synthetic biology point of view, such a boosted-stress resistance gene represents a useful BioBrick (or building block) for the design of more physiologically robust probiotics or, indeed, plants that are more resistant to cold arid conditions.
Published by Landes Bioscience since 2010, Bioengineered publishes relevant and high-impact original research with a special focus on genetic engineering that involves the generation of recombinant strains and their metabolic products for beneficial applications in food, medicine, industry, environment and bio-defense. Established in 2002, Landes Bioscience is an Austin, Texas-based publisher of biology research journals and books. For more information on Landes Bioscience, please visit http://www.landesbioscience.com/.
Andrew Thompson | EurekAlert!
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences