Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Separation of DNA and proteins through improved gel electrophoresis

04.03.2014

Medical diagnoses and DNA sequencing can be made cheaper, faster and more reliable using a new miniaturized technique for gel electrophoresis based on conducting polymer materials, according to researchers at Linköping University in Sweden.

Gel electrophoresis is a process through which different proteins or DNA fragments are separated so that they can be identified and studied. Today, most separations require considerable manual work and are carried-out on large gels which require several hours to complete.

The industry needs miniaturized systems capable of automatically performing a large number of separations simultaneously, and much more quickly.

PhD students Katarina Bengtsson and Sara Nilsson from the Transport and Separations Group at Linköping University have demonstrated a significant step toward miniaturized gel electrophoresis. Their finding – recently published in the scientific journal PLoS ONE – was achieved by developing conducting polymer materials to replace platinum electrodes that are traditionally used in gel electrophoresis systems.

This advance allows the stationary metal electrodes fixed in electrophoresis equipment to be replaced. The plastic electrodes can then be included as part of a disposable cassette containing the separation gel. This eliminates cross-contamination between gels run in sequence. Other issues in electrophoresis are bubble formation and pH-changes caused by water electrolysis.

PhD student Per Erlandsson has previously shown that the conducting polymer materials are able to be oxidized and reduced themselves, thereby eliminating the need to electrolyze water in electrokinetic systems.

"One of our strategies is to find ways to use these materials, developed for the printed electronics industry, in applications other than electronics and optoelectronics. We hope that this result will accelerate the automation and miniaturization of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable," says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their "work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems."

The technology will be further developed by the university start-up company LunaMicro AB.

###

Article: Conducting Polymer Electrodes for Gel Electrophoresis av Katarina Bengtsson, Sara Nilsson och Nathaniel D. Robinson. PLoS ONE open access February 2014. http://dx.doi.org/10.1371/journal.pone.0089416.

Nathaniel Robinson | EurekAlert!
Further information:
http://www.liu.se

Further reports about: DNA Gel Linköping PLoS bubble electrodes electrophoresis eliminating materials proteins

More articles from Life Sciences:

nachricht Faster detection of pathogens in the lungs
24.06.2016 | Universität Zürich

nachricht How yeast cells regulate their fat balance
23.06.2016 | Goethe-Universität Frankfurt am Main

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First experimental quantum simulation of particle physics phenomena

Physicists in Innsbruck have realized the first quantum simulation of lattice gauge theories, building a bridge between high-energy theory and atomic physics. In the journal Nature, Rainer Blatt‘s and Peter Zoller’s research teams describe how they simulated the creation of elementary particle pairs out of the vacuum by using a quantum computer.

Elementary particles are the fundamental buildings blocks of matter, and their properties are described by the Standard Model of particle physics. The...

Im Focus: Is There Life On Mars?

Survivalist back from Space - 18 months on the outer skin of the ISS

A year and a half on the outer wall of the International Space Station ISS in altitude of 400 kilometers is a real challenge. Whether a primordial bacterium...

Im Focus: CWRU physicists deploy magnetic vortex to control electron spin

Potential technology for quantum computing, keener sensors

Researchers at Case Western Reserve University have developed a way to swiftly and precisely control electron spins at room temperature.

Im Focus: Physicists measured something new in the radioactive decay of neutrons

The experiment inspired theorists; future ones could reveal new physics

A physics experiment performed at the National Institute of Standards and Technology (NIST) has enhanced scientists' understanding of how free neutrons decay...

Im Focus: Discovery of gold nanocluster 'double' hints at other shape changing particles

New analysis approach brings two unique atomic structures into focus

Chemically the same, graphite and diamonds are as physically distinct as two minerals can be, one opaque and soft, the other translucent and hard. What makes...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ERES 2016: The largest conference in the European real estate industry

09.06.2016 | Event News

Networking 4.0: International Laser Technology Congress AKL’16 Shows New Ways of Cooperations

24.05.2016 | Event News

Challenges of rural labor markets

20.05.2016 | Event News

 
Latest News

Nanoscientists develop the 'ultimate discovery tool'

24.06.2016 | Materials Sciences

Russian physicists create a high-precision 'quantum ruler'

24.06.2016 | Physics and Astronomy

Hubble confirms new dark spot on Neptune

24.06.2016 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>