Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Separation of DNA and proteins through improved gel electrophoresis

04.03.2014

Medical diagnoses and DNA sequencing can be made cheaper, faster and more reliable using a new miniaturized technique for gel electrophoresis based on conducting polymer materials, according to researchers at Linköping University in Sweden.

Gel electrophoresis is a process through which different proteins or DNA fragments are separated so that they can be identified and studied. Today, most separations require considerable manual work and are carried-out on large gels which require several hours to complete.

The industry needs miniaturized systems capable of automatically performing a large number of separations simultaneously, and much more quickly.

PhD students Katarina Bengtsson and Sara Nilsson from the Transport and Separations Group at Linköping University have demonstrated a significant step toward miniaturized gel electrophoresis. Their finding – recently published in the scientific journal PLoS ONE – was achieved by developing conducting polymer materials to replace platinum electrodes that are traditionally used in gel electrophoresis systems.

This advance allows the stationary metal electrodes fixed in electrophoresis equipment to be replaced. The plastic electrodes can then be included as part of a disposable cassette containing the separation gel. This eliminates cross-contamination between gels run in sequence. Other issues in electrophoresis are bubble formation and pH-changes caused by water electrolysis.

PhD student Per Erlandsson has previously shown that the conducting polymer materials are able to be oxidized and reduced themselves, thereby eliminating the need to electrolyze water in electrokinetic systems.

"One of our strategies is to find ways to use these materials, developed for the printed electronics industry, in applications other than electronics and optoelectronics. We hope that this result will accelerate the automation and miniaturization of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable," says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their "work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems."

The technology will be further developed by the university start-up company LunaMicro AB.

###

Article: Conducting Polymer Electrodes for Gel Electrophoresis av Katarina Bengtsson, Sara Nilsson och Nathaniel D. Robinson. PLoS ONE open access February 2014. http://dx.doi.org/10.1371/journal.pone.0089416.

Nathaniel Robinson | EurekAlert!
Further information:
http://www.liu.se

Further reports about: DNA Gel Linköping PLoS bubble electrodes electrophoresis eliminating materials proteins

More articles from Life Sciences:

nachricht Protein Shake-Up
27.03.2015 | Oak Ridge National Laboratory

nachricht How did the chicken cross the sea?
27.03.2015 | Michigan State University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Experiment Provides the Best Look Yet at 'Warm Dense Matter' at Cores of Giant Planets

In an experiment at the Department of Energy's SLAC National Accelerator Laboratory, scientists precisely measured the temperature and structure of aluminum as...

Im Focus: Energy-autonomous and wireless monitoring protects marine gearboxes

The IPH presents a solution at HANNOVER MESSE 2015 to make ship traffic more reliable while decreasing the maintenance costs at the same time. In cooperation with project partners, the research institute from Hannover, Germany, has developed a sensor system which continuously monitors the condition of the marine gearbox, thus preventing breakdowns. Special feature: the monitoring system works wirelessly and energy-autonomously. The required electrical power is generated where it is needed – directly at the sensor.

As well as cars need to be certified regularly (in Germany by the TÜV – Technical Inspection Association), ships need to be inspected – if the powertrain stops...

Im Focus: 3-D satellite, GPS earthquake maps isolate impacts in real time

Method produced by UI researcher could improve reaction time to deadly, expensive quakes

When an earthquake hits, the faster first responders can get to an impacted area, the more likely infrastructure--and lives--can be saved.

Im Focus: Atlantic Ocean overturning found to slow down already today

The Atlantic overturning is one of Earth’s most important heat transport systems, pumping warm water northwards and cold water southwards. Also known as the Gulf Stream system, it is responsible for the mild climate in northwestern Europe. 

Scientists now found evidence for a slowdown of the overturning – multiple lines of observation suggest that in recent decades, the current system has been...

Im Focus: Robot inspects concrete garage floors and bridge roadways for damage

Because they are regularly subjected to heavy vehicle traffic, emissions, moisture and salt, above- and underground parking garages, as well as bridges, frequently experience large areas of corrosion. Most inspection systems to date have only been capable of inspecting smaller surface areas.

From April 13 to April 17 at the Hannover Messe (hall 2, exhibit booth C16), engineers from the Fraunhofer Institute for Nondestructive Testing IZFP will be...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

World Conference On Regenerative Medicine 2015: Registration And Abstract Submission Now Open

25.03.2015 | Event News

University presidents from all over the world meet in Hamburg

19.03.2015 | Event News

10. CeBiTec Symposium zum Big Data-Problem

17.03.2015 | Event News

 
Latest News

Two Most Destructive Termite Species Forming Superswarms in South Florida

27.03.2015 | Agricultural and Forestry Science

ORNL-Led Team Demonstrates Desalination with Nanoporous Graphene Membrane

27.03.2015 | Materials Sciences

Coorong Fish Hedge Their Bets for Survival

27.03.2015 | Ecology, The Environment and Conservation

VideoLinks
B2B-VideoLinks
More VideoLinks >>>