Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Separation of DNA and proteins through improved gel electrophoresis

04.03.2014

Medical diagnoses and DNA sequencing can be made cheaper, faster and more reliable using a new miniaturized technique for gel electrophoresis based on conducting polymer materials, according to researchers at Linköping University in Sweden.

Gel electrophoresis is a process through which different proteins or DNA fragments are separated so that they can be identified and studied. Today, most separations require considerable manual work and are carried-out on large gels which require several hours to complete.

The industry needs miniaturized systems capable of automatically performing a large number of separations simultaneously, and much more quickly.

PhD students Katarina Bengtsson and Sara Nilsson from the Transport and Separations Group at Linköping University have demonstrated a significant step toward miniaturized gel electrophoresis. Their finding – recently published in the scientific journal PLoS ONE – was achieved by developing conducting polymer materials to replace platinum electrodes that are traditionally used in gel electrophoresis systems.

This advance allows the stationary metal electrodes fixed in electrophoresis equipment to be replaced. The plastic electrodes can then be included as part of a disposable cassette containing the separation gel. This eliminates cross-contamination between gels run in sequence. Other issues in electrophoresis are bubble formation and pH-changes caused by water electrolysis.

PhD student Per Erlandsson has previously shown that the conducting polymer materials are able to be oxidized and reduced themselves, thereby eliminating the need to electrolyze water in electrokinetic systems.

"One of our strategies is to find ways to use these materials, developed for the printed electronics industry, in applications other than electronics and optoelectronics. We hope that this result will accelerate the automation and miniaturization of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable," says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their "work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems."

The technology will be further developed by the university start-up company LunaMicro AB.

###

Article: Conducting Polymer Electrodes for Gel Electrophoresis av Katarina Bengtsson, Sara Nilsson och Nathaniel D. Robinson. PLoS ONE open access February 2014. http://dx.doi.org/10.1371/journal.pone.0089416.

Nathaniel Robinson | EurekAlert!
Further information:
http://www.liu.se

Further reports about: DNA Gel Linköping PLoS bubble electrodes electrophoresis eliminating materials proteins

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

7th International Conference on Crystalline Silicon Photovoltaics in Freiburg on April 3-5, 2017

03.04.2017 | Event News

 
Latest News

DGIST develops 20 times faster biosensor

24.04.2017 | Physics and Astronomy

Nanoimprinted hyperlens array: Paving the way for practical super-resolution imaging

24.04.2017 | Materials Sciences

Atomic-level motion may drive bacteria's ability to evade immune system defenses

24.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>