Researchers have developed a faster and more accurate way to test for infection with Ophidiomyces ophiodiicola, a fungus that is killing snakes in the Midwest and eastern United States. The test also allows scientists to monitor the progression of the infection in living snakes.
The researchers reported on the test at the 2014 Mycological Society of America Annual Meeting.
"We need people to know that they don't have to anesthetize an animal to collect a biopsy sample or, worse yet, euthanize snakes in order to test for the infection," said University of Illinois comparative biosciences department professor Matthew Allender, an expert in snake fungal disease. "Now we can identify the infections earlier, we can intervene earlier and we can potentially increase our success of treatment or therapy."
The new test uses quantitative polymerase chain reaction (qPCR), which amplifies fungal DNA to identify the species present and measure the extent of infection.
Researchers first took notice of Ophidiomyces (oh-FID-ee-oh-my-sees) in snakes in the mid-2000s. Today the fungus threatens the last remaining eastern massasauga (mass-uh-SAW-guh) rattlesnake population in Illinois and has been found to infect timber rattlesnakes, mud snakes, rat snakes, garter snakes, milk snakes, water snakes and racers in several states, Allender said.
"I've tested snakes from Illinois, Tennessee and Michigan, and we've tested samples from snakes in New Jersey, Georgia and Virginia," Allender said. Snakes in Connecticut, Massachusetts, Minnesota, New Hampshire, New York, Ohio and Wisconsin have also tested positive for the fungus. (Watch a movie about the research)
Ophidiomyces consumes keratin, a key ingredient in snake scales. It can cause scabs, nodules, abnormal molting, ulcers and other disfiguring changes to snake skin. Mortality is 100 percent in Illinois massasauga rattlesnakes found with outward signs of infection, Allender said. There are only 100 to 150 massasaugas left in Illinois, he said, and about 15 percent of those are infected with the disease.
Allender also is an affiliate of the Illinois Natural History Survey, part of the Prairie Research Institute at the U. of I. He and his INHS colleague, mycologist Andrew Miller, liken this emerging fungal disease in snakes to white-nose syndrome, another fungal disease that has killed millions of North American bats. Miller and graduate student Daniel Raudabaugh recently published an analysis of Pseudogymnoascus destructans, the fungus implicated in white-nosed syndrome, and are repeating the analysis on Ophidiomyces.
"The fungus killing these snakes is remarkably similar in its basic biology to the fungus that has killed over 6 million bats," Miller said. "It occurs in the soil, seems to grow on a wide variety of substances, and possesses many of the same enzymes that make the bat fungus so deadly."
Other colleagues at the INHS, herpetologists Michael Dreslik and Chris Phillips, have been studying eastern massasauga rattlesnakes in the wild for 15 years, and are working closely with Allender to characterize both biological and health factors that lead to infection. The new qPCR test is integral to this mission, Allender said. It also will help the team develop new therapies to treat infections in snakes.
"This work is truly collaborative across disciplines, allowing the team to make advances in studying this disease that haven't been accomplished anywhere else," Allender said.
"Our qPCR is more than 1,000 times more sensitive than conventional PCR," Allender said. "We can know how many [fungal spores] are in a swab and then we can start to treat the snake and we can watch to see if that number is going down."
The researchers also are hoping to find new disinfectants that will kill the fungus so that researchers who are studying snakes in the wild will not spread it to new locales on their equipment or shoes.
"Some of our preliminary studies show that the common disinfectants that we use are not effective," Allender said. "This fungus overcomes it."
The Illinois Wildlife Preservation Fund Grant Program, offered through the Illinois Department of Natural Resources, provided support for this work. Funds for this program are generated through the tax check-off offered on Illinois income tax returns.
Editor's notes: To reach Matthew Allender, call 217-265-0320; email firstname.lastname@example.org.
Diana Yates | University of Illinois
Newly designed molecule binds nitrogen
23.02.2018 | Julius-Maximilians-Universität Würzburg
Atomic Design by Water
23.02.2018 | Max-Planck-Institut für Eisenforschung GmbH
A newly developed laser technology has enabled physicists in the Laboratory for Attosecond Physics (jointly run by LMU Munich and the Max Planck Institute of Quantum Optics) to generate attosecond bursts of high-energy photons of unprecedented intensity. This has made it possible to observe the interaction of multiple photons in a single such pulse with electrons in the inner orbital shell of an atom.
In order to observe the ultrafast electron motion in the inner shells of atoms with short light pulses, the pulses must not only be ultrashort, but very...
A group of researchers led by Andrea Cavalleri at the Max Planck Institute for Structure and Dynamics of Matter (MPSD) in Hamburg has demonstrated a new method enabling precise measurements of the interatomic forces that hold crystalline solids together. The paper Probing the Interatomic Potential of Solids by Strong-Field Nonlinear Phononics, published online in Nature, explains how a terahertz-frequency laser pulse can drive very large deformations of the crystal.
By measuring the highly unusual atomic trajectories under extreme electromagnetic transients, the MPSD group could reconstruct how rigid the atomic bonds are...
Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
23.02.2018 | Physics and Astronomy
23.02.2018 | Health and Medicine
23.02.2018 | Physics and Astronomy