The same processes that provide easier access to the energy-rich sugar molecules bound up in the plant's tenacious architecture are leading to innovations for the biofuels industry. The research, conducted by more than 50 authors, is reported in the February 4 online edition of the Proceedings of the National Academy of Sciences (PNAS).
Among the challenges to more cost-effective production of biofuels from cellulosic biomass—the fibrous material of whole plants—is to find effective means to work around the polymer lignin, the scaffolding that endows the plant's architecture with rigidity and protection from pests. By doing so, the organic compound cellulose—the long chain of glucose (sugar) units can be unbound, broken down, fermented, and distilled into liquid transportation fuel. This is where the destructive capabilities of rot come in.
"The microbial world represents a little explored yet bountiful resource for enzymes that can play a central role in the deconstruction of plant biomass—an early step in biofuel production," said Eddy Rubin, Director of the DOE JGI, where the genome sequencing was conducted. "The brown-rot Postia placenta's genome offers us a detailed inventory of the biomass-degrading enzymes that this and other fungi possess."
Rubin pointed to a complementary strategy that DOE and its Bioenergy Research Centers are pursuing of targeting a new generation of plants—perennial grasses and fast-growing trees such as poplar—bred specifically as biomass for biofuels. Among the desirable characteristics of biofuel "feedstocks" is the ease by which they can be deconstructed. Traditionally, harsh chemicals and expensive high-heat treatments have been employed. In parallel with the development of improved feedstocks is finding just the right mix of enzymes to get the most out of converting biomass into fuel.
"Nature offers some guidance here," said Dan Cullen, FPL scientist and one of the senior authors on the PNAS paper. "Postia has, over its evolution, shed the conventional enzymatic machinery for attacking plant material. Instead, the evidence suggests that it utilizes an arsenal of small oxidizing agents that blast through plant cell walls to depolymerize the cellulose. This biological process opens a door to more effective, less-energy intensive and more environmentally-sound strategies for more lignocellulose deconstruction."
Few organisms in nature can efficiently breakdown lignin into smaller, more manageable chemical units amenable to biofuels production. The exceptions are the basidiomycete fungi, which include white-rot and brown-rot—wood-decayers and essential caretakers of carbon in forest systems. In addition, brown-rot fungi have significant economic impact because their ability to wreak havoc with wooden structures. A significant portion of the U.S. timber harvest is diverted toward replacing such decayed materials.
Unlike white-rot fungi, previously characterized by DOE JGI and FPL, which simultaneously degrades lignin and cellulose, brown-rot rapidly depolymerizes the cellulose in wood without removing the lignin. Up until this study, the underlying genetics and biochemical mechanisms were poorly understood.
DNA sequence is the first step in the central dogma of molecular biology first articulated over 50 years ago by Francis Crick—the transfer of information from DNA to RNA, which in turn, is translated into protein products, such as enzymes. Postia's genome sequence was also the first step in the process that the scientific team employed to home in on the subset of data, the transcriptome, that encodes the specific enzyme activity, and the secretome, the products exported from the cell.
"For the first time we have been able to compare the genetic blue prints of brown-rot, white-rot and soft-rot fungi which play a major role in the carbon cycle of our planet," said Randy Berka, another one of the study's senior authors and Director of Integrative Biology, at Novozymes, Inc., of Davis, Calif. "Such comparisons will increase our understanding of the diverse mechanisms and chemistries involved in lignocellulose degradation. This type of information may empower industrial biotechnologists to devise new strategies to enhance efficiencies and reduce costs associated with biomass conversion for renewable fuels and chemical intermediates."
Further reports about: > DOE > Glucose > Lignin > PNAS > Postia placenta's genome > Production > biochemical machinery > biofuels industry > biofuels production > brown-rot > brown-rot fungi > cellulosic biomass > energy-rich sugar molecules > enzymes > fibrous material > fungi > fungi possess > genetic code > liquid transportation fuel > pests > polymer lignin > renewable fuel
Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory
How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
24.03.2017 | Materials Sciences
24.03.2017 | Physics and Astronomy
24.03.2017 | Physics and Astronomy