Researchers in Japan have launched FANTOM5, Functional Annotation of the Mammalian Genome, an international effort to globally map transcription initiation in every human cell type.
Joint research by RIKEN and Helicos BioSciences Corporation has played a key role in this project in adapting the Cap Analysis of Gene Expression (CAGE) technique, originally developed by RIKEN, to the HeliscopeTM single molecule sequencer. The use of HeliscopeTM for CAGE completely avoids PCR amplification biases, is quantitative over 5 orders of magnitude, is highly reproducible and can be carried out on as little as 100ng of total RNA.
The FANTOM project is the brainchild of Yoshihide Hayashizaki, who launched the first phase of the project in 2000. The cDNA encyclopedia of mouse full-length cDNAs generated in the FANTOM1, 2 and 3 projects remains to this day the largest collection of mammalian full-length cDNAs. FANTOM3 provided insights into non-coding RNAs and sense-antisense regulation. It also introduced the CAGE technique, developed by Piero Carninci, which generates sequence tags from the 5’ ends of capped RNAs.
In FANTOM4, CAGE was applied to an acute myeloid leukemia cell line undergoing monocytic differentiation. Using CAGE and transcription factor binding site predictions, a transcriptional regulatory model was generated which identified the key transcription factors involved in monocytic differentiation. FANTOM5 takes this one huge leap further by trying to generate transcriptional regulatory models to define every human cell type.
Motivating the project is the idea that to build a full understanding of transcriptional regulation in a human system, we need to collect as large a set of diverse cellular states as possible. Different cellular states will express different subsets of genes, which in turn must be regulated by different combinations of transcription factors. While a large collection of human primary cell types has already been amassed for the project, many more are still needed. Potential collaborators working on rare cell types are invited to contact Alistair Forrest, who is co-coordinating sample collection for the project.For more information, please contact:
RIKEN Omics Science Center is one of 12 research centers in RIKEN and its focus is on developing genome-wide technologies and applications thereof.
A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
On the way to developing a new active ingredient against chronic infections
21.08.2017 | Deutsches Zentrum für Infektionsforschung
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
21.08.2017 | Materials Sciences
21.08.2017 | Health and Medicine
21.08.2017 | Materials Sciences