Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers Use Light To Move Molecules

18.03.2011
Using a light-triggered chemical tool, Johns Hopkins scientists report that they have refined a means of moving individual molecules around inside living cells and sending them to exact locations at precise times.

This new tool, they say, gives scientists greater command than ever in manipulating single molecules, allowing them to see how molecules in certain cell locations can influence cell behavior and to determine whether cells will grow, die, move or divide. A report on the work was published online December 13 in the Journal of the American Chemical Society.

Studying how just one signaling molecule communicates in various parts of a living cell has posed a challenge for scientists investigating how different interactions influence cell behavior, such as the decision to move, change shape or divide.

“By using one magical chemical set off by light, we modified our previous technique for moving molecules around and gained much more control,” says Takanari Inoue, Ph.D., assistant professor of cell biology and member of the Center for Cell Dynamics in the Institute for Basic Biomedical Sciences. “The advantage of using light is that it is very controllable, and by confining the light, we can manipulate communication of molecules in only a tiny region of the cell,” he says.

Specifically, the Hopkins team designed a way to initiate and spatially restrict the molecular interactions to a small portion of the cell by attaching a light-triggered chemical to a bulky molecule, the bond between which would break when researchers shined a defined beam of ultraviolet light on it. This enabled the chemical to enter the cell and force two different and specific proteins in that cell to mingle when they otherwise wouldn’t. Normally, these proteins would have nothing to do with each other without the presence of the light-triggered chemical, but researchers decided to take advantage of this mingling to explore how certain proteins in a cell behave when transported to precise locations.

Next, researchers modified the two mingling proteins by attaching special molecules to them — one sent one of the proteins to the edge of the cell and another caused ripples to form on the edge of the cell — so that if ripples form on the edge of the cell, they would know that the proteins were interacting there.

The researchers put both modified proteins inside human skin cells and bathed the cells in the light-triggered chemical tool. Then, they shone a tiny UV beam directed on approximately ten percent of the edge of a skin cell. Ripples appeared only on the region of the cell near where the light was beamed, demonstrating that the tool could limit cell activity to a precise location in the cell.

The tool can be used in larger cells, Inoue says, to monitor as little as one percent of a specific molecule if the beam intensity is varied. That in turn could reveal in even more detail the secret affairs of proteins in cellular cubbyholes.

“With this technique, we can get a finer understanding of cell function on the molecular level,” says Inoue. “Our technique allows us to monitor whatever molecule we choose in whichever tiny space we choose so that we can understand how a molecule functions in a specific part of a live cell.”

This study was funded by the National Institutes of Health and fellowships from the Japan Society for the Promotion of Science.

Other authors on this manuscript are Nobuhiro Umeda, Tasuku Ueno and Christopher Pohlmeyer, and Tetsuo Nagano of The University of Tokyo.

On the web:
Takanari Inoue http://www.hopkinsmedicine.org/cellbio/dept/InoueProfile.html
Department of Cell Biology http://www.hopkinsmedicine.org/cellbio/dept/index.html

Journal of the American Chemical Society http://pubs.acs.org/journal/jacsat

| Newswise Science News
Further information:
http://www.hopkinsmedicine.org/cellbio/dept/index.html
http://pubs.acs.org/journal/jacsat

More articles from Life Sciences:

nachricht A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

nachricht On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

A Map of the Cell’s Power Station

18.08.2017 | Life Sciences

Engineering team images tiny quasicrystals as they form

18.08.2017 | Physics and Astronomy

Researchers printed graphene-like materials with inkjet

18.08.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>