Researchers from Thomas Jefferson University, the Pasteur Institute in Paris, and Yale University reported in PLoS ONE, a way in which intracellular pathogens exploit the biological attributes of their hosts in order to escape destruction.
Intracellular pathogens include Chlamydia, which causes infertility in women, and Legionella, which causes Legionnaire’s disease. These pathogens are able to escape destruction and remain in the cells. Until now, it was unclear how they were able avoid the destruction process. The team of researchers, led by Fabienne Paumet, Ph.D., assistant professor of Microbiology and Immunology at Jefferson Medical College of Thomas Jefferson University, found that it appears to be due to SNARE-like proteins expressed by the pathogen.
SNARE proteins are necessary for eukaryotic cells to fuse to their intracellular compartments. These proteins, which are present on the surface of almost all intracellular compartments, interact to form a stable complex, triggering fusion of the membranes. Intracellular pathogens, like Chlamydia and Legionella, must contend with vesicular trafficking and membrane fusion in the host cell. But they manage to bypass the lysosome, where other pathogens would normally be destroyed.
The researchers tested the hypothesis that SNARE-like proteins expressed by the bacteria themselves were capable to interact with the eukaryotic SNAREs and alter membrane fusion to their advantage. The Chlamydia bacteria expressed a SNARE-like protein called IncA and the Legionella expressed a SNARE-like protein called IcmG/DotF, both of which inhibit SNARE-protein-mediated fusion.
“Based on our results, it seems that intracellular bacteria are able to express ‘inhibitory SNAREs’ to block fusion between the lysosome and the compartment containing the bacteria,” Dr. Paumet said. “The SNARE proteins function like a zipper, and without each half, they can’t fuse.”
SNARE-like bacterial proteins would appear to be a viable therapeutic target, since disruption of their protective function should render intracellular bacteria more susceptible to clearance from the phagosome.
“Thorough understanding of the bacterial SNARE-like protein system will give us the necessary tools to design such therapeutics,” Dr. Paumet said.
Emily Shafer | Newswise Science News
The birth of a new protein
20.10.2017 | University of Arizona
Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
20.10.2017 | Information Technology
20.10.2017 | Materials Sciences
20.10.2017 | Interdisciplinary Research