The work is reported in the Journal of the Royal Society Interface.
Using the approach they call "Direct Molecular Recognition," the UCLA and NYU researchers used nanoparticles to turn the DNA molecules into a form of molecular braille that can be read in the scale of nanometers, or one billionth of a meter, using high-speed Atomic Force Microscopy (AFM).
The leaders of the study are: Jason Reed, a research professor, and Professor Jim Gimzewski, nanotechnology pioneer, both at UCLA's California Nanosystems Institute, and Professor Bud Mishra, genomics expert, at NYU's Courant Institute of Mathematical Sciences. This group believes the method will have many practical uses, such as super-sensitive detection of DNA molecules in genomic research and medical diagnostics as well as in identifying pathogens.
While there are a variety of techniques currently used for this purpose, they are time consuming, technically difficult, and expensive. They also require a significant amount of genetic material in order to make accurate readings and often require prior knowledge of the sample composition.
According to Mishra, to overcome these shortcomings, the team devised a "single-cell, single-molecule" method that would dispense with the complex chemical manipulations on which existing methods are based, and, instead, utilize the unique shapes of the molecules themselves as the method of identification. This approach has the benefits of being rapid and sensitive to the level of a single molecule.
Reed says that "the long term goal of our team's research is to dissect, understand, and control the biology of single cells in complex tissues, such as brain, or in malignant tumors. Furthering this body of work requires that we address an unsolved problem in single-cell molecular analysis: the lack of a method to routinely, reliably, and inexpensively determine global gene transcriptional activity."
James Devitt | EurekAlert!
New risk factors for anxiety disorders
24.02.2017 | Julius-Maximilians-Universität Würzburg
Stingless bees have their nests protected by soldiers
24.02.2017 | Johannes Gutenberg-Universität Mainz
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
24.02.2017 | Life Sciences
24.02.2017 | Life Sciences
24.02.2017 | Trade Fair News