Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers clone key sperm-binding proteins

19.03.2009
University of Montreal and Maisonneuve-Rosemont Hospital Research Center study published in the journal Molecular Human Reproduction

New treatments for infertility could be closer to reality, thanks to a discovery from scientists at the Université de Montréal and Maisonneuve-Rosemont Hospital Research Centre.

According to a study published in the journal Molecular Human Reproduction, the researchers have become the first to clone, produce and purify a protein important for sperm maturation, termed Binder of Sperm (BSP), which may have implications for both fertility treatments and new methods of male contraception.

"We have previously isolated and characterized BSPs from many species, such as bulls and boars," says Dr. Puttaswamy Manjunath, senior author and a professor in the departments of medicine and of biochemistry at the Université de Montréal and a member of the Maisonneuve-Rosemont Hospital Research Centre.

"We know from these studies that if this protein is missing or defective in these species, fertility is compromised. We believe that BSP is equally important in humans."

An elusive protein
Dr. Manjunath and colleagues have tried to isolate human BSPs for more than 10 years. In most mammals, these proteins are typically produced by the seminal vesicles and added to sperm at ejaculation. Yet this is not the case for humans, primates and rodents. According to Dr.Manjunath and his team, these species produce small amounts of BSPs only in the epididymis, a duct that connects the testes to the urethra.

"For a few years, we were looking in the wrong place," says Dr. Manjunath. "In addition, the minute quantities of BSP produced in humans has made it impossible to isolate and characterize."

Cloning leads to purification

Dr. Manjunath and his team went back to the basics. Using molecular biology technique they cloned the gene (DNA) that encodes human BSP. Through cloning, they were able to produce and purify this protein.

"After considerable troubleshooting, we were able to produce functional human BSP. Our next steps are to confirm its biological role in human fertility," says Dr. Manjunath.

Role of BSPs in other animals

Following ejaculation, sperm undergo a complex series of modifications inside the female reproductive tract. The changes sperm undergo during this process include redistribution of surface proteins, loss of sperm membrane lipids and increased sperm movement. A family of sperm-binding proteins (BSPs) secreted by the seminal vesicles has been shown to be essential for sperm maturation in female reproductive tracts of cows, sheep, pigs and other hoofed animals.

Sylvain-Jacques Desjardins | EurekAlert!
Further information:
http://www.umontreal.ca

More articles from Life Sciences:

nachricht Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery
20.01.2017 | GSI Helmholtzzentrum für Schwerionenforschung GmbH

nachricht Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>