While cell replacement therapies can be effective, the challenge is to deliver a sufficient quantity of healthy cells, said Boyd Evans III of the lab’s Measurement Science and Systems Engineering Division.
“Regardless of the source of cells and the location of delivery, there is a great need to improve cell viability after the cells are transplanted,” Evans said. “The vast majority of transplanted cells do not survive more than 24 hours regardless of their source.”
Studies have shown that merely implanting more cells does not necessarily increase the number that survive and differentiate into dopamine-producing, or viable, cells in Parkinson’s models. The key is being able to deliver precise quantities of healthy cells to a targeted location. This requires the ability to determine if the cells are viable upon delivery and the ability to make meaningful measurements. ORNL’s proprietary instrumented cell delivery catheter allows physicians to do just that.
“Our approach consists of monitoring cells that are implanted using a catheter equipped with a fiber optic probe to perform fluorescence-based cytometric measurements on cells as they exit the port at the catheter tip,” Evans said. These measurements confirm that the cell is alive and provide indications of the cell’s health.
“What we have done is taken the function of a laboratory instrument and put it on the tip of a catheter that can make measurements inside the brain,” Evans said.
Results from several studies underscore the value of delivering a highly controlled amount of tissue into the host brain, and understanding cell viability at the delivery point is critical for meaningful comparison of experimental results, according to Evans.
The instrumented catheter is part of a larger effort to develop a complete system for collecting healthy tissue from an individual who is both the donor and recipient, expanding this tissue in vitro and implanting the tissue under monitored conditions. Joining Boyd in this effort are other researchers from ORNL, George Gillies of the University of Virginia, and neurosurgeon William Broaddus and neuroscientist Helen Fillmore of Virginia Commonwealth University.
Funding provided through ORNL’s Laboratory Directed Research and Development program was used to develop a prototype device and demonstrate its functionality for characterizing cell flows of cell.
Following completion of the LDRD funded project, NexGen Medical Systems and Kopf Family Foundation at the University of Virginia, and the Cullather, Hord and Hafner Funds at Virginia Commonwealth University worked on other issues associated with flows of slurries of cells, such as cell delivery, cell selection and culturing technique.
ORNL is managed by UT-Battelle for the Department of Energy.
Ron Walli | Newswise Science News
Zap! Graphene is bad news for bacteria
23.05.2017 | Rice University
Discovery of an alga's 'dictionary of genes' could lead to advances in biofuels, medicine
23.05.2017 | University of California - Los Angeles
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...
In the race to produce a quantum computer, a number of projects are seeking a way to create quantum bits -- or qubits -- that are stable, meaning they are not much affected by changes in their environment. This normally needs highly nonlinear non-dissipative elements capable of functioning at very low temperatures.
In pursuit of this goal, researchers at EPFL's Laboratory of Photonics and Quantum Measurements LPQM (STI/SB), have investigated a nonlinear graphene-based...
Dental plaque and the viscous brown slime in drainpipes are two familiar examples of bacterial biofilms. Removing such bacterial depositions from surfaces is...
23.05.2017 | Event News
22.05.2017 | Event News
17.05.2017 | Event News
23.05.2017 | Physics and Astronomy
23.05.2017 | Life Sciences
23.05.2017 | Medical Engineering