Cells have devised many structures for transporting molecular cargo across their protective borders, but the nuclear pore complex, with its flower-like, eight-fold symmetry, stands out. Monstrously large by cellular standards, as well as versatile, this elaborate portal controls access to and exit from the headquarters of the cell, the nucleus.
In research published June 4 in Cell, Rockefeller University scientists have uncovered crucial steps in the dynamic dance that dilates and constricts the nuclear pore complex -- the latest advance in their ongoing efforts to tease apart the mechanism by which its central channel admits specific molecules. Their work, based on quantitative biophysical data, has revealed that the nuclear pore complex is much more than the inert structure it was once thought to be.
The octagonal center ring of the nuclear pore complex opens when nucleoporin 58 (red) interacts with nucleoporin 54 (blue), then constricts as they separate. New research shows unstructured regions of 58 (squiggly filaments) interact with transport factors (green). This interaction makes the dilated conformation more favorable, pushing the ring to open.
Credit: The Laboratory of Cell Biology
"Prevailing wisdom cast the nuclear pore complex as a rigid conduit. Instead, we have found that it responds to the need for transport, opening and closing in an elegantly simple cycle," says study author Gunter Blobel, John D. Rockefeller Jr. Professor and head of the Laboratory of Cell Biology.
"Our most recent study reveals how proteins called transport factors, known to chaperone legitimate cargo through the nuclear pore complex, prompt the ring at the middle of the central channel to dilate."
More than a billion years ago, certain cells gained an evolutionary advantage by surrounding their DNA in a protective membrane, creating the nucleus. However, this innovation created a problem: How to move molecules, in some cases very large ones, in and out.
The nuclear pore complex was one solution, first described at Rockefeller over 50 years ago by Michael Watson, a postdoc in the Palade-Porter Laboratory. Years later, Blobel's lab identified the first of the proteins that act as the complex's building blocks: nucleoporins. For some time, it has been assumed that unstructured portions of some nucleoporin molecules guarded the complex's rigid central channel by creating a sort of gel-like barrier.
But ongoing work in Blobel's lab indicates the central channel is anything but rigid. In previous research, a team from his lab identified a flexible ring in the middle of the central channel, the diameter of which was determined by two of the nuclear pore complex's approximately 30 nucleoporins, Nup58 and Nup54, which associate and disassociate from one another in what the researchers dubbed the ring cycle.
When these two nucleoporins associate, the ring dilates to a diameter of up to 50 nanometers, a size capable of accommodating a ribosomal subunit, the largest and most complex of the cell's molecular freight. Then, when the nucleoporins separate, the single ring divides into three rings with diameters of 20 nanometers.
This most recent research, conducted by postdoc Junseock Koh in Blobel's laboratory, examines how a transport factor known as karyopherin initiates the dilation of the ring. To accomplish this, Koh measured changes in heat during reactions between the three components, karyopherin, Nup58, and Nup54.
Biophysical data like this reveals the energy dynamics during these reactions, and so provides clues to the behavior of the molecules involved. To tease apart this complex system, Koh mathematically analyzed the data collected at various conditions. His results revealed an unexpected role for a disordered region of Nup58.
"We found that when one karyopherin molecule binds to at least two disordered regions of Nup58, it stabilizes Nup58 in such a way that the dilated conformation -- in which the neighboring ordered region of Nup58 links up with Nup54 -- becomes more favorable. As a result, the more karyopherin molecules are present, the more the ring dilates," Koh says. "Based on these results, we were able to develop a framework for predicting the extent to which a ring will dilate given the amount of transport factors present."
"While this discovery is a critical step in understanding how the nuclear pore complex opens and closes, its implications go beyond basic cell biology", says Blobel, who is also Investigator of the Howard Hughes Medical Institute. "It is likely that even subtle problems in nuclear pore complex function may over time lead to numerous diseases."
Wynne Parry | EurekAlert!
Complementing conventional antibiotics
24.05.2018 | Goethe-Universität Frankfurt am Main
Building a brain, cell by cell: Researchers make a mini neuron network (of two)
23.05.2018 | Institute of Industrial Science, The University of Tokyo
A research team led by physicists at the Technical University of Munich (TUM) has developed molecular nanoswitches that can be toggled between two structurally different states using an applied voltage. They can serve as the basis for a pioneering class of devices that could replace silicon-based components with organic molecules.
The development of new electronic technologies drives the incessant reduction of functional component sizes. In the context of an international collaborative...
At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.
At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...
There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?
At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
24.05.2018 | Ecology, The Environment and Conservation
24.05.2018 | Medical Engineering
24.05.2018 | Physics and Astronomy