Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

How cells keep in shape - Scientists elucidate a molecular mechanism that plays a key role in giving a cell its shape

03.12.2007
Cells in our body come in various shapes and sizes. Each cell is shaped in such a way as to optimise it for a specific function. When things go wrong and a cell does not adopt its dedicated shape, its function can be impaired and the cell can cause problems in the body.

Researchers at the European Molecular Biology Laboratory (EMBL) and the Institute for Atomic and Molecular Physics (AMOLF), The Netherlands, have now decoded a molecular mechanism that plays an important role in the development of a cell’s shape. In this week’s issue of Nature they report a new experimental approach that sheds light on the interaction between proteins and the cell’s skeleton.

That each cell type has its unique shape is due to its cytoskeleton, an internal scaffold built of protein filaments. Especially important are microtubules, dynamic filaments that constantly grow and shrink. Their spatial organisation inside cells depends on a variety of regulator proteins, some of which only interact with the growing ends of these filament. How these so called plus-end tracking proteins recognise the dynamic structure of a growing microtubule end is a long-standing puzzle. Researchers in the groups of Thomas Surrey and Damian Brunner at EMBL and of Marileen Dogetrom at AMOLF have now developed the first method that allows to simultaneously study multiple plus-end tracking molecules, so called +TIPs, in the test tube.

“+TIPs specifically bind to the fast-growing plus end of a microtubule and follow it as it grows. They act as a plus-end label so that other proteins know where to bind to regulate the filament’s stability,” says Surrey. “For years it has been impossible to reconstitute this behaviour in a test tube. Our new system now revealed which proteins need to be present for plus-end tracking and what the underlying mechanisms are.”

... more about:
»Filament »Tracking »microtubule »plus-end

Applying the new method they succeeded in dissecting a minimal molecular system consisting of three end tracking proteins from yeast cells. The proteins were labelled with fluorescence to monitor their behaviour with a microscope. This procedure revealed that one of the proteins has the ability to recognise the specific structure of the growing microtubule tip, binds to it and acts as a loading platform for the other two proteins. The inherent motor activity of one of the other two proteins, which allows it to walk along microtubules, contributes to the ability of the molecular system to follow growing microtubule ends selectively.

“The great advantage of our new assay is that it can be applied to all kinds of other proteins that interact with microtubules,” says Peter Bieling, who carried out the research in Surrey’s lab. “It is a powerful approach that will advance our understanding of the large variety of different microtubule end tracking proteins and can shed light on their mechanics and functions.”

Anna-Lynn Wegener | alfa
Further information:
http://www.embl.org/downloads/

Further reports about: Filament Tracking microtubule plus-end

More articles from Life Sciences:

nachricht Bolstering fat cells offers potential new leukemia treatment
17.10.2017 | McMaster University

nachricht Ocean atmosphere rife with microbes
17.10.2017 | King Abdullah University of Science & Technology (KAUST)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Ocean atmosphere rife with microbes

17.10.2017 | Life Sciences

Neutrons observe vitamin B6-dependent enzyme activity useful for drug development

17.10.2017 | Life Sciences

NASA finds newly formed tropical storm lan over open waters

17.10.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>