Researchers at the European Molecular Biology Laboratory (EMBL) and the Institute for Atomic and Molecular Physics (AMOLF), The Netherlands, have now decoded a molecular mechanism that plays an important role in the development of a cell’s shape. In this week’s issue of Nature they report a new experimental approach that sheds light on the interaction between proteins and the cell’s skeleton.
That each cell type has its unique shape is due to its cytoskeleton, an internal scaffold built of protein filaments. Especially important are microtubules, dynamic filaments that constantly grow and shrink. Their spatial organisation inside cells depends on a variety of regulator proteins, some of which only interact with the growing ends of these filament. How these so called plus-end tracking proteins recognise the dynamic structure of a growing microtubule end is a long-standing puzzle. Researchers in the groups of Thomas Surrey and Damian Brunner at EMBL and of Marileen Dogetrom at AMOLF have now developed the first method that allows to simultaneously study multiple plus-end tracking molecules, so called +TIPs, in the test tube.
“+TIPs specifically bind to the fast-growing plus end of a microtubule and follow it as it grows. They act as a plus-end label so that other proteins know where to bind to regulate the filament’s stability,” says Surrey. “For years it has been impossible to reconstitute this behaviour in a test tube. Our new system now revealed which proteins need to be present for plus-end tracking and what the underlying mechanisms are.”
Applying the new method they succeeded in dissecting a minimal molecular system consisting of three end tracking proteins from yeast cells. The proteins were labelled with fluorescence to monitor their behaviour with a microscope. This procedure revealed that one of the proteins has the ability to recognise the specific structure of the growing microtubule tip, binds to it and acts as a loading platform for the other two proteins. The inherent motor activity of one of the other two proteins, which allows it to walk along microtubules, contributes to the ability of the molecular system to follow growing microtubule ends selectively.
“The great advantage of our new assay is that it can be applied to all kinds of other proteins that interact with microtubules,” says Peter Bieling, who carried out the research in Surrey’s lab. “It is a powerful approach that will advance our understanding of the large variety of different microtubule end tracking proteins and can shed light on their mechanics and functions.”
Anna-Lynn Wegener | alfa
WPI team grows heart tissue on spinach leaves
23.03.2017 | Worcester Polytechnic Institute
Inactivate vaccines faster and more effectively using electron beams
23.03.2017 | Fraunhofer-Institut für Organische Elektronik, Elektronenstrahl- und Plasmatechnik FEP
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
23.03.2017 | Power and Electrical Engineering
23.03.2017 | Earth Sciences
23.03.2017 | Life Sciences