Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Imaging neural progenitor cells in the living human brain

12.11.2007
For the first time, investigators have identified a way to detect neural progenitor cells (NPCs), which can develop into neurons and other nervous system cells, in the living human brain using a type of imaging called magnetic resonance spectroscopy (MRS). The finding, supported by the National Institutes of Health (NIH), may lead to improved diagnosis and treatment for depression, Parkinson's disease, brain tumors, and a host of other disorders.

Research has shown that, in select brain regions, NPCs persist into adulthood and may give rise to new neurons. Studies have suggested that the development of new neurons from NPCs, called neurogenesis, is disrupted in disorders ranging from depression and schizophrenia to Parkinson's disease, epilepsy, and cancer. Until now, however, there has been no way to monitor neurogenesis in the living human brain.

"The recent finding that neural progenitor cells exist in adult human brain has opened a whole new field in neuroscience. The ability to track these cells in living people would be a major breakthrough in understanding brain development in children and continued maturation of the adult brain. It could also be a very useful tool for research aimed at influencing NPCs to restore or maintain brain health," says Walter J. Koroshetz, M.D., deputy director of the NIH's National Institute of Neurological Disorders and Stroke (NINDS), which helped fund the work. The study was also funded by the NIH's National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK).

"This is the first noninvasive approach to identify neural progenitor cells in the human brain," says Grigori Enikolopov, Ph.D., of Cold Spring Harbor Laboratory in New York, who conducted the new study along with co-corresponding author Mirjana Maletic-Savatic, M.D., Ph.D., of the State University of New York, Stony Brook and their colleagues at SUNY Stony Brook and Brookhaven National Laboratory. MRS is an imaging technique that can be used to detect proteins and other compounds normally present in body fluids or tissues. The study results are published in the November 9, 2007, issue of Science.*

... more about:
»Brain »Human »Living »MRS »NPC »human brain »neural »progenitor

Previously developed techniques using positron emission tomography and other types of brain imaging allow investigators to identify NPCs in animals. However, those techniques require pre-labeling the cells with radioactive agents or magnetic nanoparticles – strategies that are not practical in people. In the new study, the researchers identified an innate property of NPCs that can be detected by MRS. This enables them to image NPCs without introducing drugs or other agents.

The researchers used a technique related to MRS to compare the signals of NPCs from embryonic mice to those of neurons, astrocytes, and oligodendrocytes. Astrocytes and oligodendrocytes are non-neuronal cells that are very common in the brain. The investigators found that NPCs showed a specific signal, or marker, that was not as common in other cell types.

Next, the researchers studied NPCs at various points as they differentiated into other cell types in the laboratory. The level of the NPC signal decreased over time, while the levels of other markers common in neurons and astrocytes rose. The newly identified marker was more common in brain cells from embryonic mice than in those from adult mice. It also was more common in cells from the mouse hippocampus, a region where neurogenesis occurs constantly, than in cells from the brain's cortex, where new neurons are not normally formed.

Dr. Maletic-Savatic, Dr. Enikolopov and their colleagues then gave adult mice a form of electrical stimulation that increases the amount of neurogenesis in the brain. They found that the marker they had identified increased significantly after the stimulation. Additional results indicated that the marker is probably a mixture of lipids (fatty acids), although the exact identity of the lipids, and how they function in NPCs, is still undetermined.

The researchers then developed a signal processing method that allowed them to separate the marker from other signals in the living brain. They transplanted NPCs into the cortex of the adult rat brain and found that they could clearly detect the marker in the area where the NPCs were injected. They also found that it increased after stimulation.

Finally, the investigators tested their MRS imaging technique in healthy people. They found major differences in the concentration of the marker between the hippocampus and the cortex. They also imaged the brains of pre-adolescents, adolescents, and adults and found that the marker decreased with age.

The findings suggest that the marker identified in these experiments can be used to detect NPCs and neurogenesis in the live human brain using MRS. They also show that NPCs decrease during brain development. Previous research had shown that neurogenesis decreases with age in animals, but this is the first study to demonstrate that it also decreases in the living human brain.

"This study identifies a novel biomarker and shows that we can use it to see progenitor cells in the live brain," Dr. Enikolopov says. "This protocol can now be used to study a variety of problems." For example, researchers might study people with depression to see if neurogenesis correlates with alterations in depression or schizophrenia. The technique might also be used to study changes that occur in neurological diseases such as traumatic brain injury, stroke, epilepsy, and Parkinson's disease. It might even be useful for detecting cancer, because researchers believe some brain tumors are associated with aberrant proliferation of NPCs, Dr. Enikolopov adds.

The researchers are now planning studies that will test the usefulness of the new imaging technique in people with disease. They also hope to improve their understanding of how the lipids they detected function in NPCs and to refine the sensitivity of their technique.

Natalie Frazin | EurekAlert!
Further information:
http://www.ninds.nih.gov/
http://www.niddk.nih.gov
http://www.nih.gov

Further reports about: Brain Human Living MRS NPC human brain neural progenitor

More articles from Life Sciences:

nachricht Making fuel out of thick air
08.12.2017 | DOE/Argonne National Laboratory

nachricht ‘Spying’ on the hidden geometry of complex networks through machine intelligence
08.12.2017 | Technische Universität Dresden

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

Im Focus: Successful Mechanical Testing of Nanowires

With innovative experiments, researchers at the Helmholtz-Zentrums Geesthacht and the Technical University Hamburg unravel why tiny metallic structures are extremely strong

Light-weight and simultaneously strong – porous metallic nanomaterials promise interesting applications as, for instance, for future aeroplanes with enhanced...

Im Focus: Virtual Reality for Bacteria

An interdisciplinary group of researchers interfaced individual bacteria with a computer to build a hybrid bio-digital circuit - Study published in Nature Communications

Scientists at the Institute of Science and Technology Austria (IST Austria) have managed to control the behavior of individual bacteria by connecting them to a...

Im Focus: A space-time sensor for light-matter interactions

Physicists in the Laboratory for Attosecond Physics (run jointly by LMU Munich and the Max Planck Institute for Quantum Optics) have developed an attosecond electron microscope that allows them to visualize the dispersion of light in time and space, and observe the motions of electrons in atoms.

The most basic of all physical interactions in nature is that between light and matter. This interaction takes place in attosecond times (i.e. billionths of a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

Blockchain is becoming more important in the energy market

05.12.2017 | Event News

 
Latest News

New research identifies how 3-D printed metals can be both strong and ductile

11.12.2017 | Physics and Astronomy

Scientists channel graphene to understand filtration and ion transport into cells

11.12.2017 | Materials Sciences

What makes corals sick?

11.12.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>