Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Massive project reveals shortcomings of modern genome analysis

08.11.2007
The sequencing and comparison of 12 fruit fly genomes -- the result of a massive collaboration of hundreds of scientists from more than 100 institutions in 16 countries -- has thrust forward researchers' understanding of fruit flies, a popular animal model in science. But even human genome biologists may want to take note: The project also has revealed considerable flaws in the way they identify genes.

"We've made huge progress in recent years with many genomes, including humans, but a lot of the problems can't be solved by simply dumping data into a computer and having truth and light come out the other end," said Indiana University Bloomington biologist Thomas Kaufman, who co-led the project. "One of the things we've learned from this project is that when you compare a lot of different but related genomes, you are more likely to see the genes that are buried in all that A-C-T-G mush."

Two papers in this week's Nature separately report the results of the four-year genome project and use the data to draw some conclusions about the fruit fly genus Drosophila, particularly its star species, the human nuisance Drosophila melanogaster. Among the papers' conclusions is the idea that resolving any individual species' genome is greatly enhanced when related genomes are compared to it. The project was primarily funded by the National Human Genome Research Institute (NHGRI), part of the National Institutes of Health.

More than 40 "companion" manuscripts are being published or are in press, each of which examines a different aspect of the data produced by the Drosophila 12 Genomes Consortium.

... more about:
»Drosophila »Genome »RNA »species

"This remarkable scientific achievement underscores the value of sequencing and comparing many closely-related species, especially those with great potential to enhance our understanding of fundamental biological processes," said Francis S. Collins, director of NHGRI. "Thanks to the consortium's hard work, scientists around the world now have a rich new source of genomic data that can be mined in many different ways and applied to other important model systems as well as humans."

The consortium purposely chose a wide variety of fruit flies for study, guessing correctly that both gene similarities and differences among the 12 species would be easier to identify. Some of the Drosophila species the scientists studied are closely related to D. melanogaster, some not. Some of the flies fulfill very specialized ecological niches, such as D. sechellia, which has evolved a unique ability to detoxify the fruit of the Seychelles' noni tree. The other 10 species the consortium examined were D. pseudoobscura, D. simulans, D. yakuba, D. erecta, D. ananassae, D. persimilis, D. willistoni, D. virilis, D. grimshawi, and the cactus-loving D. mojavensis. D. melanogaster's genome was published in 2000 and D. pseudoobscura's genome was published in 2005. The other genomes are newly published.

In comparing the 12 genomes, the scientists found 1,193 new protein-coding genes and hundreds of new functional elements, including regulatory sequences that determine how quickly genes are expressed, and genes that encode functional RNAs such as small nuclear RNAs. They also learned certain genes appear to be evolving faster than others, such as the genes associated with smell and taste, sex and reproduction, and defenses against pathogens.

The Drosophila 12 Genomes Consortium found that D. melanogaster shares about 77 percent of its genes with the other 11 species they studied. The scientists also found errors in about 3 percent of previously sequenced D. melanogaster protein-coding genes, correcting 414 gene sequences on record.

A vexing problem for genomicists is finding genes and other important DNA sequences in heterochromatin, tightly packed areas of chromosomes presumed to experience little expression. Heterochromatin is common in animal genomes.

"The heterochromatin is very hard to analyze," Kaufman said. "Studies show heterochromatin changes the most. It's full of intermediate- and full-repeat sequences. And there are genes buried in this stuff."

The conventions for locating the genes that encode proteins are pretty well established. The lingering problem for genomics biologists is locating genes whose parts are interrupted repeatedly, as well as locating genes that do not code for proteins.

By comparing a huge number of genomes, these sorts of genes are relatively easy to locate. Genes that do important things for cells or tissues are more likely to be "conserved" over time; that is, they don't change much despite millions of years of mutations.

One of the companion pieces accompanying this week's Nature papers was written by IUB computational biologist Matthew Hahn. Hahn reports in PLoS Genetics that although all 12 Drosophila species have about the same number of genes (14,000), the genomes are more dynamic than one might expect.

"The highest turnover in gene number occurs in genes involved in sex and reproduction," Hahn said. "Our results demonstrate that the apparent stasis in total gene number among species has masked rapid turnover in individual gene gain and loss. It is likely that this evolutionary revolving door has played a large role in shaping the morphological, physiological, and metabolic differences among species. This is the reason the 12 species only share 77 percent of their genes."

David Bricker | EurekAlert!
Further information:
http://www.indiana.edu

Further reports about: Drosophila Genome RNA species

More articles from Life Sciences:

nachricht Multi-institutional collaboration uncovers how molecular machines assemble
02.12.2016 | Salk Institute

nachricht Fertilized egg cells trigger and monitor loss of sperm’s epigenetic memory
02.12.2016 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

Im Focus: Molecules change shape when wet

Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water

In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...

Im Focus: Fraunhofer ISE Develops Highly Compact, High Frequency DC/DC Converter for Aviation

The efficiency of power electronic systems is not solely dependent on electrical efficiency but also on weight, for example, in mobile systems. When the weight of relevant components and devices in airplanes, for instance, is reduced, fuel savings can be achieved and correspondingly greenhouse gas emissions decreased. New materials and components based on gallium nitride (GaN) can help to reduce weight and increase the efficiency. With these new materials, power electronic switches can be operated at higher switching frequency, resulting in higher power density and lower material costs.

Researchers at the Fraunhofer Institute for Solar Energy Systems ISE together with partners have investigated how these materials can be used to make power...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

UTSA study describes new minimally invasive device to treat cancer and other illnesses

02.12.2016 | Medical Engineering

Plasma-zapping process could yield trans fat-free soybean oil product

02.12.2016 | Agricultural and Forestry Science

What do Netflix, Google and planetary systems have in common?

02.12.2016 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>