Wilhelmson used gene technology to study the role of oxygen in the growth and hyoscyamine production of Egyptian henbane hairy root cultivations and in the malting of barley.
Hyoscyamine is a pharmaceutical compound that is mainly isolated from plants because other production methods are not economically feasible. Instead of isolation from plants, hyoscyamine could be produced in hairy root cultivations of Egyptian henbane; however, in industrial scale processes oxygen transfer to the roots becomes difficult.
Barley is the main raw material in beer production. The lack of oxygen has traditionally been believed to restrict the germination of barley in the malting process and therefore aeration is used during the process. However, oxygen added through aeration does not necessarily reach the barley embryo because it may be consumed by microbes and in chemical reactions.
Wilhelmson transferred the vhb gene encoding an efficient haemoglobin from the Vitreoscilla microbe to Egyptian henbane and barley. The hypothesis was that the haemoglobin would improve growth and hyoscyamine production of the Egyptian henbane root cultures and the germination rate of barley. In addition, she studied the effect of aeration on the processes.
The study showed that the haemoglobin of Vitreoscilla improved the growth of root cultures but did not have a significant effect on hyoscyamine production. The effect of Vitreoscilla haemoglobin was not identical to that of aeration indicating that mechanisms other than those directly related to oxygen concentration are involved. The results will help in further development of pharmaceutical production from hairy roots.
The importance of oxygen deficiency in malting was highly dependent on the stage of the process. Wilhelmson proved that oxygen deficiency is a natural and inevitable stage in barley germination, and aeration did not reduce the oxygen deficiency of barely during the early stages of germination. Moreover, Vitreoscilla haemoglobin did not improve germination; on the contrary, it even slowed down germination slightly. A certain degree of oxygen deficiency may actually be favourable for germination because it generates nitric oxide, which accelerates germination. The research showed that the need for aeration increases as the malting process proceeds. Malt houses can utilise the results in timing the aeration of the malting process.
VTT, Research Scientist Annika Wilhelmson, M.Sc, . will defend her doctoral thesis on 12 October 2007 at noon at the Helsinki University of Technology (Street address: Kemistintie 1, Auditorium Ke2, Espoo, Finland).
Press Office | alfa
Link Discovered between Immune System, Brain Structure and Memory
26.04.2017 | Universität Basel
Researchers develop eco-friendly, 4-in-1 catalyst
25.04.2017 | Brown University
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
27.04.2017 | Earth Sciences
27.04.2017 | Materials Sciences
27.04.2017 | Materials Sciences