The technique is based on the transmission of tiny electric current in a very thin film sample cell containing a colony of bacteria. The current produces electrolysis that changes the local pH level in the vicinity of the electrodes. The bacteria, uncomfortable with the changes in pH, swim away from the electrodes and ultimately congregate in the middle of the experimental cell. Concentrated bacteria form self-organized swirls and jets resembling vortices in vigorously stirred fluid.
The method, which is suitable for flagellated bacteria such as E. coli, Bacillus subtilis, among many others, relies on the ability of bacteria to swim toward areas of optimal pH level. The bacteria live in an environment of a specific pH level, so that an increase or decrease of pH stimulates the bacteria to avoid areas of non-comfortable pH and swim in the direction of pH gradient. The researchers used an electric current to create a controlled deviation of the pH levels from the bulk values. Since only living bacteria respond to the pH stimulation, using this method can separate living and dead cells or bacteria with different motility.
The device, capable to change the thickness of a film from 1mm to 1 micron (with accuracy of 5 percent) and control the position of electrodes, is intended to separate and concentrate small quantities of living and dead microorganisms in confined spaces. It can be used for the purposes of express bioanalysis, diagnostic and identification of small bacterial samples, and separation sicken/live cells. A patent for the device is currently pending.
“Using this method, our research succeeded in dramatically increasing the concentration of microorganisms in tiny fluid drops and films. Unlike traditional centrifuging techniques, the new approach allows selective concentration of healthy cells,” said Andrey Sokolov, Ph.D. student from Illinois Institute of Technology and contributor to the research.
In addition to the development of the device used in the experimentation, research findings uncovered the explanation for the long-standing fundamental questions on the properties of collective and organized motion in the systems of interacting self-moving objects. Besides swimming bacteria, other examples include bird flocks, fish schools, motor proteins in living cell, and even swarms of communicating nano-robots.
“We have presented experimental studies of collective bacterial swimming in thin fluid films where the dynamics are essentially two-dimensional and the concentration can be adjusted continuously,” explained Igor Aronson, physicists at Materials Science Division, Argonne National Laboratory. “Our results provide strong evidence for the pure hydrodynamic origin of collective swimming, rather than chemotactic mechanisms of pattern formation when microorganisms just follow gradients of a certain chemical, such as nutrient, oxygen, or other.”
Detailed results of these findings have been published in Physical Review E and in Physical Review Letters.
Funding for this research was provided by the U.S. Department of Energy's Office of Basic Energy Science.
The nation's first national laboratory, Argonne National Laboratory conducts basic and applied scientific research across a wide spectrum of disciplines, ranging from high-energy physics to climatology and biotechnology. Since 1990, Argonne has worked with more than 600 companies and numerous federal agencies and other organizations to help advance America's scientific leadership and prepare the nation for the future. Argonne is managed by UChicago Argonne, LLC for the U.S. Department of Energy's Office of Science.
For more information, please contact Sylvia Carson (630/252-5510 or email@example.com) at Argonne.
Sylvia Carson | alfa
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