Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Bypassing eggs, flu vaccine grown in insect cells shows promise

12.04.2007
An experimental flu vaccine made in insect cells – not in eggs, where flu vaccines currently available in the United States are grown – is safe and as effective as conventional vaccines in protecting people against the flu, according to results published in the April 11 issue of the Journal of the American Medical Association.

Removing eggs from the flu vaccine manufacturing process is one option for health officials seeking to protect the population from seasonal flu as well as a potential bird-flu pandemic. Using eggs to grow vaccine takes time; a flu vaccine that relies on a different technology is capable of being produced in large amounts much more quickly, a key advantage if a bird flu pandemic were to occur.

“Eggs can be very cumbersome to work with,” said John Treanor, M.D., the flu expert at the University of Rochester Medical Center who led the study of 460 people reported in JAMA. “When you need hundreds of millions of fertilized eggs, you’re dealing with a whole host of agricultural issues, as well as scientific concerns regarding the flu virus itself. Flu viruses can be temperamental, and it’s not always an easy matter to get the virus to grow as you want in eggs.”

The use of cell culture systems to grow vaccines – using viruses as tiny factories to churn out mass amounts of vaccines – is a growing business. A similar technology using human cell lines is used to produce the hepatitis B vaccine, while one form of a vaccine against human papilloma virus is made using the same insect cell line used in the JAMA study.

... more about:
»Treanor »bird flu »flu »pandemic

In the study conducted by Treanor, together with colleagues at Cincinnati Children’s Hospital and the University of Virginia, scientists tested a vaccine called FluBlOk that is made by Protein Sciences Corp. of Meriden, Ct. FluBlOk relies on a virus known as baculovirus, which normally infects insects, to churn out the key components of the flu virus in a cell line drawn from caterpillars.

In the study funded by the company of 460 healthy people ages 18 to 49, one-third of the participants received a smaller dose of the vaccine (75 micrograms), one-third received a larger dose (135 micrograms), and one-third received a placebo shot that didn’t include vaccine. Each of the “real” shots included vaccine designed to protect against the three strains of flu that had been predicted to be the greatest threat during the 2004-2005 winter, when the study was conducted.

As the scientists expected, both the smaller dose and the larger dose caused an immune reaction generally considered effective for fighting off the flu, with the larger dose creating a stronger immune response. The side effects of the vaccine were the same as those usually reported from a typical flu shot – mainly mild arm pain.

Then, in the months that followed, there were seven cases of flu in the group that had not received the vaccine, compared to two cases in the group that received the smaller dose, and no cases in the group that received the larger dose. Together, the two vaccines reduced flu infection rate by 86 percent.

“Even though the study was small, the results are very promising,” said Treanor, who is professor of Medicine and of Microbiology and Immunology and director of the Vaccine and Treatment Evaluation Unit at the University of Rochester. “While we certainly hoped and expected the vaccine to be protective, you don’t know that until you actually test it. We’ve shown that the vaccine does work in the real world.”

Freedom from the egg brings implications important to a world facing the threat of pandemic bird flu.

For decades the nation’s efforts to prevent flu have centered on growing flu virus in hundreds of millions of fertilized eggs, with each egg containing less than a teaspoonful of material that will ultimately become part of a vaccine. It’s typically a six-month process to produce enough flu vaccine to protect the public.

Taking eggs out of the process would likely slice one or two months off the production process, Treanor said. In case of a bird-flu pandemic, that would allow manufacturers to ramp up vaccine production more quickly than if they had to wait for the production of millions of eggs. Not relying on chicken eggs might also be advisable in case a bird flu pandemic hits chicken flocks hard. The insect-cell technology also simplifies the manufacturing process in another way: A live flu virus is needed when growing vaccine in eggs, a danger when working with a potent bird-flu strain.

The technology would also help make it possible to boost the dose that patients receive, by increasing the nation’s capacity to churn out vaccine. That’s especially crucial in the fight against bird flu, as Treanor and other scientists have shown that an experimental vaccine appears to be effectively only at high doses.

The experimental vaccine differs from approved vaccines in another way as well. The experimental vaccine focuses on a portion of the flu virus known as the hemagglutinin, which the virus uses to attach to blood cells. Unlike conventional vaccines, FluBlOk does not also include neuraminidase, an enzyme that allows a flu virus to replicate and spread. While the hemagglutinin is the focus of most vaccines, scientists have been curious to measure how a vaccine without neuraminidase performs.

Tom Rickey | EurekAlert!
Further information:
http://www.urmc.rochester.edu

Further reports about: Treanor bird flu flu pandemic

More articles from Life Sciences:

nachricht Water forms 'spine of hydration' around DNA, group finds
26.05.2017 | Cornell University

nachricht How herpesviruses win the footrace against the immune system
26.05.2017 | Helmholtz-Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>