Functional characterization of SUCNR1, a G-protein coupled receptor with succinate as its specific ligand, suggests that SUCNR1 is part of a novel and physiologically relevant agonist pathway in platelet activation.
“The Bloodomics discovery is very important, as it helps us understand links between platelets and clot formation”, explains Dr. Willem Ouwehand, Bloodomics Project Coordinator and Director of Research and Planning of the ECGF (European Cardiovascular Genetics Foundation). “Now we are trying to understand the impact this and other novel receptors have on the development of heart disease, with the ultimate aim to better prevent heart disease in the future.”
To identify previously unknown platelet receptors Bloodomics compared the transcriptomes of in vitro differentiated megakaryocytes (MKs) and erythroblasts (EBs). RNA was obtained from purified, biologically paired MK and EB cultures and compared using cDNA microarrays.
Bioinformatical analysis of MK upregulated genes identified 151 transcripts encoding transmembrane domain containing proteins. Whilst many of these were known platelet genes, a number of previously unidentified, or poorly characterized, transcripts were also detected. Many of these transcripts, including G6b, G6f, LRRC32, LAT2 and the G-protein coupled receptor SUCNR1, encode proteins with structural features or functions that suggest they may be involved in the modulation of platelet function.
Immunoblotting on platelets confirmed the presence of the encoded proteins, and flow cytometric analysis confirmed the expression of G6b, G6f and LRRC32 on the surface of platelets. Through comparative analysis of expression in platelets and other blood cells the researchers demonstrated that G6b, G6f and LRRC32 are restricted to the platelet lineage, whereas LAT2 and SUCNR1 were also detected in other blood cells.
The identification of the succinate receptor SUCNR1 in platelets is of particular interest, as physiologically relevant concentrations of succinate were shown to potentiate the effect of low doses of a variety of platelet agonists.
The Bloodomics project aims at discovering genetic markers for the prediction of thrombus formation in coronary artery disease and at designing better anti-thrombotics for improved prevention and treatment. The Bloodomics project focuses on the genetics and cell biology of platelets, since it hypothesizes that the response of platelets to plaque rupture is critical in determining whether thrombus formation will lead to arterial blood vessel occlusion.
Silvia Novembre | alfa
Researchers identify potentially druggable mutant p53 proteins that promote cancer growth
09.12.2016 | Cold Spring Harbor Laboratory
Plant-based substance boosts eyelash growth
09.12.2016 | Fraunhofer-Institut für Angewandte Polymerforschung IAP
Physicists of the University of Würzburg have made an astonishing discovery in a specific type of topological insulators. The effect is due to the structure of the materials used. The researchers have now published their work in the journal Science.
Topological insulators are currently the hot topic in physics according to the newspaper Neue Zürcher Zeitung. Only a few weeks ago, their importance was...
In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.
Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...
Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
09.12.2016 | Life Sciences
09.12.2016 | Ecology, The Environment and Conservation
09.12.2016 | Health and Medicine