Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Imaging techniques permit scientists to follow a day -- or four -- in the life of a cell

08.01.2007
Methods to visualize live cells are highlighted in the current release of Cold Spring Harbor Protocols

The movement and growth of cells are critical for normal physiological processes, and--when perturbed--can result in negative outcomes such as tumor formation. Understanding how live cells function is therefore invaluable for molecular and cellular biologists, and advanced techniques to visualize cells in action are of great importance.

The current issue of Cold Spring Harbor Protocols (www.cshprotocols.org) addresses these concerns with two freely accessible protocols: one for inserting 'reporter' proteins into cells to monitor what's going on inside, and another for maintaining the cells under a microscope for long-term observation.

The first protocol, available at

... more about:
»Microscope »technique
http://www.cshprotocols.org/cgi/content
/full/2007/1/pdb.prot4657, details a procedure to inject proteins into individual cells. The injected protein can be fluorescently labeled, so scientists can track the glowing protein with a microscope and observe what it's doing and where it's going inside the cell. This microinjection technique is similar to those that are used to create cloned and transgenic organisms, and it can also be applied to more complex assays--including laser photobleaching and fluorescent speckle microscopy--that yield specific insights into cellular processes.

A second freely available protocol

(http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4660) outlines the construction and use of an enclosed microscope chamber. This device is designed to optimize the culture environment for cells--allowing them to grow as they would normally--while optimizing the conditions for viewing them by microscopy. This enables researchers to monitor the cells under a microscope for long time periods (in excess of four days), during which the cells may divide multiple times and cycle through nearly all cellular functions. When coupled with the latest in image-analysis software, this technique allows researchers to gain a long-term perspective on events in the lives of cells.

Maria Smit | EurekAlert!
Further information:
http://www.cshl.edu

Further reports about: Microscope technique

More articles from Life Sciences:

nachricht Hunting pathogens at full force
22.03.2017 | Helmholtz-Zentrum für Infektionsforschung

nachricht A 155 carat diamond with 92 mm diameter
22.03.2017 | Universität Augsburg

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Pulverizing electronic waste is green, clean -- and cold

22.03.2017 | Materials Sciences

Astronomers hazard a ride in a 'drifting carousel' to understand pulsating stars

22.03.2017 | Physics and Astronomy

New gel-like coating beefs up the performance of lithium-sulfur batteries

22.03.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>