Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A fisheye view of the deadliest breast cancer

14.12.2006
A transgenic xenografted minnow puts aggressive breast cancer under the glass. All breast cancers are not created equal.

Inflammatory breast cancer (IBC) is the deadliest form of the disease, with fewer than half of those diagnosed today having a five-year prognosis for survival. To find out what drives this most aggressive of human breast cancers, and to rapidly screen for drugs that might stop IBC, researchers at the University of California, San Diego (UCSD), have come up with an unlikely yet extremely promising ally: a transparent fish suitable for xenografts.

More properly, it’s a transgenic zebrafish, a tropical minnow native to streams around the Bay of Bengal and common to aquariums around the world, which was chemically treated to be immune suppressed. In recent years, the zebrafish has vaulted to the top as a laboratory model organism. The zebrafish is tough, easy to breed, and a vertebrate, just like us. Being immune suppressed, the transgenic fish allowed Konstantin Stoletov and colleagues at UCSD to insert a xenograft of human MDA breast cancer cells. Being transparent, the zebrafish’s tissues gave them a window on live human cancer cells in action.

The researchers were particularly interested in the small GTPase RhoC that is overexpressed in highly metastatic forms of breast cancer. Using various fluorescent tags, they labeled human MDA breast cancer cells in two ways to mark the parental MDA cells and to mark tumor cells that overexpress RhoC. They injected both tumor cell lines into the immune-suppressed fish and watched the cancer’s progress over several weeks. The tumor cells homed in on blood vessels, forming tumor-like aggregates and tapping into the fish’s circulatory system by inducing an angiogenic response.

... more about:
»MDA »breast cancer »cancer cells »tumor cells

A separate fluorescent labeling allowed the researchers to simultaneously monitor the parental MDA cancer cells and the RhoC-overexpressing tumor cells in the context of the fish vasculature using high-resolution, multicolor confocal microscopy. While the parental MDA cells formed tightly packed aggregates, RhoC-overexpressing cells scattered within the fish tissue. RhoC overexpression also increased tumor cell membrane dynamics leading to continuous shedding of small cellular fragments.

The transparent fish also let the researchers make high-resolution 3D images of two potential anticancer compounds at work: a vascular endothelial growth factor (VEGF) receptor inhibitor called SU5416, and a protein kinase inhibitor called PP1. Analysis of compound and mock-treated animals revealed that SU5416 leads to shrinkage of tumor cells and tumor-induced vasculature, while PP1 mainly affects tumor cell survival.

In all, the researchers were delighted with their breast cancer model’s ability to work as a real-time study window into IBC tumor behavior and as a rapid screening system for anticancer drugs. In the race against aggressive breast cancers, Stoletov and colleagues see their xenografted, genetically engineered zebrafish as a potential front runner.

John Fleischman | EurekAlert!
Further information:
http://www.ascb.org

Further reports about: MDA breast cancer cancer cells tumor cells

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

Im Focus: Using graphene to create quantum bits

In the race to produce a quantum computer, a number of projects are seeking a way to create quantum bits -- or qubits -- that are stable, meaning they are not much affected by changes in their environment. This normally needs highly nonlinear non-dissipative elements capable of functioning at very low temperatures.

In pursuit of this goal, researchers at EPFL's Laboratory of Photonics and Quantum Measurements LPQM (STI/SB), have investigated a nonlinear graphene-based...

Im Focus: Bacteria harness the lotus effect to protect themselves

Biofilms: Researchers find the causes of water-repelling properties

Dental plaque and the viscous brown slime in drainpipes are two familiar examples of bacterial biofilms. Removing such bacterial depositions from surfaces is...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

Innovation 4.0: Shaping a humane fourth industrial revolution

17.05.2017 | Event News

 
Latest News

Turmoil in sluggish electrons’ existence

23.05.2017 | Physics and Astronomy

“Pregnant” Housefly Males Demonstrate the Evolution of Sex Determination

23.05.2017 | Life Sciences

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

VideoLinks
B2B-VideoLinks
More VideoLinks >>>