Knowledge of the three-dimensional organization of tissues and organ is of key importance in most life sciences.
Traditional biological imaging techniques are limited by several factors, such as the optical properties of the tissue and access to biological markers. A major challenge in this connection has been the creation of three-dimensional images of the expressions of specific genes and proteins in large biological preparations. It has been equally complicated to try to measure the mass/volume of cells or structures that express a specific protein in a specific organ, for instance.
The study now presented, under the direction of Associate Professor Ulf Ahlgren at the Umeå Center for Molecular Medicine (UCMM, www.umu.se/ucmm), in collaboration with Dr. James Sharpe at the Centre for Genomic Regulation in Barcelona and Professor Dan Holmberg at the Department of Medical Bioscience, Umeå University, describes an elaboration of the technology for optical projection tomography (OPT) that the two former researchers helped describe in the journal Science four years ago. The scientists have combined improvements in sample processing and tomographic data processing to develop a new method that make it possible to create 3D images of specifically dyed preparation that are one centimeter in size, of organs from adult mice and rats, for example.
Moreover, the authors describe how the new method can be used to automatically measure the number and volume of specifically dyed structures in large biological preparations. The technique requires no specially developed biological marker substances; instead, it makes use of antibodies that are in routine use in many research laboratories. The article exemplifies the potential of the technology by following the degradation of insulin-producing cells in intact pancreases from a mouse model for type-1 diabetes. In this case they demonstrate a direct connection between the volume of the remaining insulin-producing cells and the development of symptoms of diabetes.
The researchers project that it will be possible to use their method to address a great number of medical and biological issues. This may include such diverse fields as the formation of blood vessels in tumor models, the analysis of biopsies taken from patients (in cirrhosis of the liver, for instance), and autoimmune infiltration processes.
Other co-authors of the article are Tomas Alanentalo, Amir Asayesh, Christina Lorén (all UCMM) and Harris Morrison (MRC, HGU, Edinburgh).
For more information, please contact Ulf Ahlgren by e-mail at firstname.lastname@example.org or by phone at +46 90-785 44 34 or cell phone at +46 70-220 92 28.
Image 1: (hjarna.jpg): Virtual clipping of OPT imaged intact mouse brain (2 days post coitum) labeled for neuronal marker Isl1.Image 2 (bukspottkortlar.jpg)
Bertil Born | idw
Designer cells: artificial enzyme can activate a gene switch
22.05.2018 | Universität Basel
Flow of cerebrospinal fluid regulates neural stem cell division
22.05.2018 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?
At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...
A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.
The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...
Cardiovascular tissue engineering aims to treat heart disease with prostheses that grow and regenerate. Now, researchers from the University of Zurich, the Technical University Eindhoven and the Charité Berlin have successfully implanted regenerative heart valves, designed with the aid of computer simulations, into sheep for the first time.
Producing living tissue or organs based on human cells is one of the main research fields in regenerative medicine. Tissue engineering, which involves growing...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
22.05.2018 | Life Sciences
22.05.2018 | Life Sciences
22.05.2018 | Life Sciences